Jurnal Biotek Medisiana Indonesia
Vol 2, No 2 (2013)

Diterima: 8 April 2013 Direvisi: 6 Mei 2013 Disetujui: 20 Agustus 2013 59 Kloning Fragmen DNA Pengkode Integrase (int) HIV (Human Immunodeficiency Virus) 1 Pada Escherichia Coli JM109

Hutapea, Hotma ( Biomedical Research Center and Development Papua)
Oktavian, Antonius ( Biomedical Research Center and Development Papua)



Article Info

Publish Date
12 Aug 2015

Abstract

Human Immunodeficiency Virus (HIV) is an RNA virus. It is a lentivirus, a retrovirus member family which causes Acquired Immunodeficiency Syndrome (AIDS). An early event in every retroviral multiplication is the integration of the viral double-stranded DNA genome into the host chromosome. The integration is facilitated by the activation of integrase.The goal of this research is to obtain the HIV integrase encoding gene. The obtained integrase ORF was intended to be cloned into cloning vector pJETclone and to transform Escherichia coli JM109. The cDNA synthesis was conducted by reverse transcription by converting the RNA genome to DNA. The obtained cDNA was amplified by Polymerase Chain Reaction (PCR) technique to obtain integrase encoding gene. The PCR product was inserted into the plasmid using blunt ended cloning system and was characterized using DNA gel electrophoresis and nucleotide analysis. The DNA gel electrophoresis of PCR product showed the expected band. Further characterization was conducted using nucleotide sequencing showed that the PCR product was homologue to HIV-1 integrase from Indonesia. The PCR was performed on the cloned showed DNA insert on expected size. The nucleotide analysis was conducted on the pure recombinant plasmid, the DNA was read properly.Key words: HIV-1,Iintegrase, E.coli JM109 AbstrakHIV (Human Immunodeficiency Virus) adalah virus RNA, dan termasuk ke dalam lentivirus, anggota kelompok retrovirus yang menyebabkan penyakit AIDS Acquired Immunodeficiency Syndrome. Tahap awal multiplikasi setiap retrovirus adalah integrasi genom DNA untai ganda virus ke dalam kromosom inang. Tahap integrasi ini difasilitasi oleh aktivasi enzim integrase. Tujuan penelitian ini adalah untuk memperoleh DNA pengkode integrase HIV. Kerangka baca terbuka atau Open Reading Frame (ORF) pengkode integrase yang diperoleh diklon ke vektor kloning pJETclone dan digunakan untuk mentransformasi Escherichia coli JM109. Sintesis cDNA dilakukan dengan mentranskripsi balik genom RNA menjadi DNA yang selanjutnya digunakan sebagai templat untuk amplifikasi ORF pengkode integrase dengan teknik reaksi polimerisasi berantai atau Polymerase Chain Reaction (PCR). Produk PCR selanjutnya disisipkan kedalam plasmid menggunakan sistem kloning blunt-ended dan dikarakterisasi dengan elektroforesis DNA dan analisis nukleotida. Analisis dengan elektroforesis DNA menunjukkan bahwa produk PCR berukuran sesuai dengan ukuran teoritis. Karakterisasi lebih lanjut dengan teknik analisis nukleotida menunjukkan produk PCR tersebut homolog dengan integrase HIV-1 dari Indonesia. Teknik PCR juga dilakukan terhadap klon, dan adanya produk PCR berukuran sesuai dengan ukuran teoritis menunjukkan adanya DNA sisipan. Analisis nukleotida dilakukan pada plasmid rekombinan murni dan DNA terbaca dengan baik.Keywords: HIV-1,Integrase, E.coli JM109

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Journal Info

Abbrev

jbmi

Publisher

Subject

Health Professions

Description

Jurnal Biotek Medisiana Indonesia published 2 times a year. This journal is a medium of information and research results and development areas for non-communicable diseases and public health program managers, as well as a means of communication the researchers /enthusiasts in the field of ...