Jurnal Biologi Tropis
Vol. 23 No. 4 (2023): October - December

Identification of Lactic Acid Bacteria From Pandan Civet Feces (P. hermaphroditus) in West Kalimantan Based on Phenotypic Similarity

Ageresya Ester Evelin Br Sibarani (Universitas Tanjungpura)
Rahmawati Rahmawati (Universitas Tanjungpura)
Firman Saputra (Universitas Tanjungpura)



Article Info

Publish Date
01 Sep 2023

Abstract

Lactic acid bacteria are included in the gram positive bacteria that are widely distributed in the digestive tract of living things, such as in the feces of the Pandan Luwak (P. hermaphroditus) and important role in improving the microbial balance in the body. The purpose of this study was to obtain phenotypic characters, types of bacteria and similarity relationship between lactic acid bacteria from feces of Pandan Civet (P. hermaphroditus) in West Kalimantan and lactic acid bacteria in the identification book Bergey's manual of determinative bacteriology. This research was conducted by the method of isolation and screening bacteria using MRSA media supplemented with CaCO3 1%. Characterization of lactic acid bacteria based on morphological, biochemical, and physiological characters in Bergey's Manual of Determination Bacteriology. The results of the analysis using the MVSP (Multivariant Statistic Package) program with SSM coefficients and the UPGMA method obtained 44 bacterial isolates  thought to belong to two genera, namely the genus Lactobacillus and Bifidobacterium with three species, namely L. casei, L. brevis and B. bifidum. The results of the analysis showed that 12 bacterial isolates had a similarity index of 87,6% to L. casei, 17 bacterial isolates had a similarity index of 92,2% to B. bifidum and 15 bacterial isolates had a similarity index of 84,5% to L. brevis.

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Journal Info

Abbrev

JBT

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi ...