Journal of Biodiversity and Biotechnology
Vol 1, No 1 (2021)

Genetic of Salak Pondoh, Gading Varieties and Its Hybrids Based on RAPD Markers

Nandariyah Nandariyah (Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret)
Parjanto Parjanto (Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret)
Pinaka Pinasti Ratu (Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret)

Article Info

Publish Date
30 Jun 2021

Abstract

A molecular marker of parent and offspring is used to find fast and accurate markers influenced by DNA isolation and amplification. This research aims to find the most suitable DNA isolation and DNA  amplification methods. This study used four DNA isolation methods; namely IM01, IM02, IM03, and IM04. DNA amplification used ten protocols (AP01, AP02, AP03, AP04, AP05, AP06, AP07, AP08, AP09, and AP010). The results of the research showed that the most suitable DNA isolation method for salak was  IM0, and the most suitable DNA amplification for salak was AP04 that produces the highest value of DNA bands. Keywords: DNA isolation; DNA amplification; hybrids.

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Journal Info
Journal of Biodiversity and Biotechnology
Website

Abbrev

jbb

Publisher

Universitas Sebelas Maret

Subject

Agriculture, Biological Sciences & Forestry

Description

Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, ...