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Journal of Biodiversity and Biotechnology
Published by Universitas Sebelas Maret
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 31 Documents
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Identication and Classification of Menoreh Durians Based on Molecular - RAPD Markers Nandariyah - Nandariyah; Endang - Yuniastuti
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.59401

Abstract

Menoreh durian is an accessions durian fruit originally from Kulonprogo district, Special Region of Yogyakarta, Indonesia. In the context of proposing Menoreh durian to be a new superior variety, it is necessary to have an identity and position in the molecular genetic group. The aim of the study was to obtain the identity and position of the of Menoreh Durian  in the molecular genetic classification. The wet laboratory experiment was using PCR-based RAPD method. Eleven accessions of durian were identified and characterized then grouped based on molecular analysis which were pink Menoreh and 3 Yellow Menoreh accsessions, Petruk, Sunan, Sukun, Sitokong, Montong, Kani and Aspar. The RAPD method was carried out in stages: DNA extraction, DNA quantity and quality test, amplification, electroforesis, visualization and analysis of results. The RAPD classification of 11 durian accessions , resulted in three groups; namely group I which consisted of Menoreh Kuning, Petruk, Aspar Sunan and Menoreh Jambon; Group II consisted of Montong, Sitokong, and Kani from Thailand; and group III consisted of only Sukun accession. Based on this grouping, Menoreh Kuning dan Menoreh Jambon which were separated from other groups at a genetic distance of more than 0.25 indicated that the accession of Menoreh Kuning and Menoreh Jambon only as variety.  
Role of ZA and KCl Fertilizer on Growth and Yield of Red Ginger (Zingiber officinale var. Rubrum) Supriyono Supriyono; A Y Darmawan; S Nyoto; M T S Budiastuti
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.74436

Abstract

Red ginger is one of the commodities much needed by the people of Indonesia. The increase in public demand has not been matched by an increase in red ginger production. Efforts to increase red ginger production can be done with effective fertilization. This study aims to determine the appropriate dose of ZA+KCl fertilization for good growth and yield of red ginger. This research was conducted in March-August 2021 in the experimental area of Pelem, Wonorejo, Jatiyoso, Karanganyar, Central Java with coordinates 7°43'24.7”S 111°05'31.2”E with an altitude of 762  ASL. This study used a Randomized Completed Block Design (RCBD) with one factor and 4 treatments, namely D0 (control), D1 (ZA 225 kg.ha-1+KCl 50kg.ha-1), D2 (ZA 450 kg.ha-1+ KCl 100 kg.ha-1) and D3 (ZA 675 kg.ha-1+KCl 150 kg.ha-1) were repeated 6 times each. The results showed that the application of several doses of fertilizer did not increase plant height, number of leaves, dry weight of straw but could increase the number of tillers per clump by 37.11% and the number of tillers per plot by 27.10%. The results of regression analysis showed that D2 had a trend to increase fresh weight and storage weight of rhizomes with an average of 1.9 t.ha-1 and 1.5 t.ha-1. This research needs to be further developed by improving environmental influences to increase the growth and yield of red ginger.
GROWTH ORCHID (♀) DENDROBIUM LINIALE X (♂) DENDROBIUM BIGGIBUM ON MEDIUM NAA AND ORGANIC MATERIAL IN VITRO Sri Hartati; Heni Purwanti; Nandariyah Nandariyah
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i2.60092

Abstract

The research aimed to study the influence of the application of NAA concentration, the addition of organic materials and a combination of both on media Knudson C towards the growth of orchid plantlet (♀) Dendrobium liniale >< (♂) Dendrobium biggibum. The purpose of using media Knudson C in culture in vitro is to optimize the growth of orchid plantlet Dendrobium liniale and Dendrobium biggibum. The experiments were carried out in the Laboratory of Plant Physiology and Biotechnology Faculty of Agriculture, Sebelas Maret University Surakarta. The research used Complete Randomized Design (CRD) with 2 treatment factors, factor I was the concentration of NAA (0 ppm, 1 ppm, 3 ppm and 5 ppm); and factor II was the organic materials (coconut water, banana, potatoes and sweet potatoes). In total there were 20 treatment combinations . Each combination was repeated four times. The results showed that the combination of sweet potato and NAA 5 ppm produced the following characteristics: the fastest root growth, most root length, and the largest number of leaves; while the combination of banana extracts and NAA 5 ppm produced a number of roots, the shoots appear most rapidly, and the highest number of shootsKeywords: genetic diversity; tissue culture; medium;
THE EFFECT OF DOSAGE AND TYPE OF FERTILIZER ON THE PERCENTAGE OF ATTACKS OF BROWN PLANTHOPPER (Nilaparvata lugens STALL.) ON SOME VARIETIES OF PADDY (Oryza sativa L.) Arkhiadi Benauli
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.62134

Abstract

The effect of dosage anf type of fertilizer on the percentage of attacks of brown planthopper (Nilaparvata lugens Stall.) on Some Varieties of Paddy (Oryza sativa L.). This study aims to determine the dose of cow manure on the percentage of brown plant hopper attack  in several varieties of paddy. This research was conducted in Desa Serdang in March-June 2021. This research used Randomized Block Design (RAK) of two factors and three replications. The first factor  is fertilizer dose consisting of 4 treatments (anorganic, 50 kg / plot, 100 kg / plot and 150 kg / plot). The other factor  is a variety consisting of 2 treatments (Inpari 10 and Inpari 32). The results showed that the dosage of 100 kg / plot cow manure was the best treatment to production per plot and resistant to the pecentage of brown plant hopper attack. Inpari 32 is the best treatment to increase production per plot and resistant to the intensity of brown plant hopper attack. The interaction of fertilizer dosage and variety (Inpari 32 + 100 kg / plot) is the best treatment to increase production per plot and resistant to the percentage of brown plant hopper attack.
In Vitro Propagation of Tribulus terrestris with IAA and BAP Concentrations Samanhudi Samanhudi; Ahmad Yunus; Rahmanto Rahmanto
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50245

Abstract

Tribulus terrestris is conventionally propagated using seeds but has limited germination capacity. One approach that can be done for this plant multiplication is in vitro tissue culture. This study was conducted to determine the growth potential of T. terrestris in vitro by the addition of auxin (IAA) and cytokinin (BAP) as growth regulators at several concentrations. The study was conducted at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret Surakarta from February to December 2017. The experiment employed factorial Completely Randomized Design (CRD) method with 2 factors, namely IAA concentration (0 ppm, 0.1 ppm, 0.2 ppm, 0.3 ppm), and BAP concentration (0 ppm, 0.3 ppm, 0.5 ppm, 0.7 ppm). The observed growth parameters were shoot emergence time, leaf emergence time, root emergence time, number of leaves, and number of roots. Data were analyzed using analysis of variance followed by DMRT with a 95% confidence level. The results showed that the addition of IAA only affects leaf emergence time, while the addition of BAP had a significant effect on the leaf emergence time and number of leaves. The interactions between IAA and BAP had a significant effect on leaf emergence time. Keywords: explant; plant growth; tissue culture.
Application Cow Manure and Mychorriza to Physiological of Biduri (Calotropis gigantea) Sri Hartati; Samanhudi Samanhudi; Muji Rahayu; Mochammad Danny Sukardan; Nadia Alifia Rahma
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.61273

Abstract

Biduri (Calotropis gigantea) was a wild plant that had many benefits in the health and textile industries. In the health sector, biduri was used as a herbal plant, while in the textile industry, used as the main raw material. Biduri could be used as a substitute for kapok as the main raw material, therefore it was necessary to develop cultivation technology to met the needs. This research was conducted at the Jumantono Field Laboratory of Faculty of Agriculture UNS, Karanganyar using a factorial Randomized Complete Block Design (RCBD), which consisted of 2 factors, namely cow dung fertilizer, and mycorrhizae. Cow dung fertilizer consists of 0 ton/ha, 10 ton/ha, and 20 ton/ha. Mycorrhizae consisted of 0 grams/plant, 5 grams/plant, 10gram/plant, and 15gram/plant. There were 36 treatment combination that consist of 12 treatment combination repeated 3 times. The interaction between cow dung did not significantly affect all physiological observation variables. The results showed that the application of 20 ton/ha of cow dung fertilizer gave the best results on the width of stomata openings, the rate of photosynthesis, and the rate of transpiration. Inoculation with mycorrhizal fungi had no significant effect on all observations of physiological variables Keywords: Calotropis gigantea, cow manure, mycorrhiza, physiological
The Effect of Concentration and Time Interval of Kinetin Application on The Growth of Daun Duduk (Desmodium triquetrum L.) Seeds Asty Pratista Magdhalena; Bambang Puji Asmanto; Sulandjari Sulandjari; Ahmad Yunus
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50418

Abstract

Daun duduk (Desmodium triquetrum L.) is a medicinal plant that has the power to treat hemorrhoids, but has not been widely cultivated. This research was conducted to obtain the right concentration and time interval of kinetin application to increase the growth of Daun duduk seedlings. This research was conducted from November 2019 until March 2020 at the Screen House, Faculty of Agriculture, UNS. The factors of the experiment were concentration of kinetin (0 ppm, 10 ppm, 20 ppm and 30 ppm) and the time interval of application (once every three months, once every 1.5 months and once a month). The parameters observed in this study were plant height, stem diameter, number of leaves, number of branches, number of roots, root length, fresh stover and dry stover. The results showed that the single factor of kinetin concentration could increase plant height, stem diameter, number of leaves, number of branches and dry stover. Meanwhile, the time interval for application and the interaction between the two factors did not have any significant effect. The most effective kinetin concentration in increasing the growth of Daun duduk was 30 ppm. Keywords: growth regulators; herbal medicines; plant height.
Integrated Sustainable Agriculture To Realize Biodiversity-Based Food and Energy Sovereignty Bambang Pujiasmanto; Desy Setyaningrum
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.61086

Abstract

Sustainable agriculture is the management and conservation of natural resources, oriented to technological and institutional changes carried out in such a way as to ensure the fulfillment and satisfaction of human needs in a sustainable manner for present and future generations. Food independence is the ability of the state and nation to produce diverse food from within the country which can ensure the fulfillment of sufficient food needs. Basically the food must be available at all times and to be able to meet the criteria for food security. Food availability is determined by 3 main aspects, namely production (quantity), distribution (accessibility), and consumption (nutritious and safe). In addition, renewable energy can come from agricultural materials. The energy crisis that occurs today is caused by an imbalance in the demand for energy that continues to increase with energy supply that is dependent on non-renewable fossil fuels, such as oil, coal and natural gas. This dependence is a serious threat to the world because of the known depletion of petroleum reserves, the instability of oil prices, and the pollution of greenhouse gases due to the burning of fossil fuels. The energy crisis will result in losses, both material and immaterial, such as loss of investor and consumer confidence, hampered creativity, inefficiency, and the risk of social unrest. The solution that is usually offered to overcome the energy crisis that occurs is the development and use of alternative energy sources, one of which is biofuels or biofuels.
EFFECT OF ALUMINIUM STRESS ON GERMINATION, GROWTH AND PHOTOSYNTHETIC PIGMENTS OF Amaranthus hybridus Musyimi Mutisya David
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.75211

Abstract

Amaranthus hybridus is widely distributed and used as a food plant in many parts of the world. Aluminium toxicity in the soil is one environmental stress that limits crop growth and production in acid soils. However, the level of aluminium stress tolerance depends on the plant species or genotype.  A study was conducted at Maseno University, Kenya on the effects of aluminium stress on germination, growth and leaf photosynthetic pigments of  Amaranthus hybridus seedlings. Ten uniform seeds of Amaranthus hybridus weretreated in sodium hypochlorite solution and were placed on dried petri dishes lined with Whatsman No. 1 filter papers.  Filter papers were then moistened with 5ml of  nutrient solution containing either 0 mM (distilled water) or (2, 4, 6, 8 and 10 mM) (Al toxicity) AlCl3·6H2O replicated three times. The germination was assessed for five days to ascertain the number of seeds that germinated over time.  Five litre pots were filled with top soil from Maseno University’s garden. Five seeds of Amaranthus hybridus were sown in each of the pots and were irrigated every day with 250 ml of tap water per pot for two weeks. After two weeks the seedlings were thinned to 2 plants per pot and treatments initiated. Seedlings were irrigated daily with nutrient solution containing 0 mM (control – tap water) or (2, 4, 6, 8 and 10 mM) (Al toxicity) AlCl3·6H2O in triplicates arranged in a completely randomized design in a green house. Data on shoot height, leaf area and number of leaves, shoot and root fresh and dry weights, chlorophyll a, chlrophyl b, total chlorophyll and carotenoid contents were determined at the end of the experiment. Data collected was subjected to analysis of variance (ANOVA) and treatment means separated and compared using Least Significance Difference (LSD). Germination percentage of Amaranthus hybridus was significantly reduced by the aluminiumsolution stress as compared to control treatment. Growth and photosynthetic pigments parameters were significantly reduced by the aluminium stress.The results of this study have clearly shown that  Amaranthus hybridus speciesis very sensitive to aluminium toxicity.
THE EFFECT USING RHIZOCTONIA MYCORRHIZAEE ON Dendrobium nandia anindhita SEEDLING R Soelistijono; K Aprilliyan; Daryanti Daryanti
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i2.56352

Abstract

Dendrobium nandia anindhita orchid is a favored hybrid of orchid because it flowers easyly and has high variety of flower colors. The aim of this study was to determine the effect of the application of Rhizoctonia mycorrhizaee on seedling Dendrobium hybrid orchids. It is expected that the seedling will have the characteristics of the Dendrobium orchid species. The research was carried out in a green house and laboratory of the Faculty of Agriculture, Tunas Pembangunan University. The design used was a completely randomized design with one variable and 25 seedling samples each. The results showed: (1) Microscopically, Rhizoctonia mycorrhizaee had the following characteristics: Fungal hyphae growing on PDA media were white, branches of Rhizoctonia mycorrhizaee hyphae formed elbows and had septa; (2) The application treatment of Rhizoctonia mycorrhizaee (M) had a significant effect on the parameters of plant height, number of leaves, number of roots, root length and plant fresh weight, treatment (M) had no significant effect on parameters of leaf length; (3) Morphological observations showed that the orchid plants that were applied to Rhizcotonia mycorrhizae had greener leaf colors, more leaves, and longer roots than those without Rhizcotonia mycorrhizae.

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