Jurnal Penelitian Pendidikan IPA (JPPIPA)
Vol. 10 No. 2 (2024): February

Deteksi Gen Resistensi Aeromonas hydrophila yang Diisolasi dari Budidaya Ikan Lele Di Jambi

titis wulandari (Universitas Muhammadiyah Muara Bungo)
Tri Wiyoko (Universitas Muhammdiyah Muara Bungo)
Tri Wera Agrita (Universitas Muhammdiyah Muara Bungo)
Nabila Swarna Puspa Hermana (Institut Pertanian Bogor)
Agustin Indrawati (Institut Pertanian Bogor)



Article Info

Publish Date
25 Feb 2024

Abstract

Aeromonas hydrophila is one of the causative agents for Motile Aeromonas Septicemia (MAS) in catfish. MAS treatment is generally carried out by administering antibiotics. This study aimed to determine the antibiotic resistance profile of A. hydrophila and detect the presence of resistance genes. The samples are archived A. hydrophila isolates isolated from catfish ponds in the district of Muara Jambi, Bungo, Jambi Province, and Merangin total of 18 isolates. Testing the antibiotic resistance profile was carried out using the disk diffusion method against tetracycline, oxytetracycline, ampicillin, amoxicillin, ciprofloxacin, enrofloxacin, and streptomycin. The detected resistance genes were ampC, qnrA, qnrB, and qnrS. The results showed A. hydrophila isolates were resistant to tetracycline (100%), oxytetracycline (94.42%), ampicillin (77.70%), and amoxicillin (77.72%) and still sensitive to ciprofloxacin (83.32%), enrofloxacin (100 %), and streptomycin (100%). The ampC gene was found in 8 out of 14 isolates resistant to beta-lactam antibiotics (57.14%). The qnrA and qnrS genes were found in 3 out of 4 isolates resistant to quinolone antibiotics (75%), while qnrB was found in 1 isolate (25%). A. hydrophila isolated from catfish ponds in Jambi develops resistance to antibiotics and resistance genes found in isolates resistant to antibiotics.

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Journal Info

Abbrev

jppipa

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

Description

Science Educational Research Journal is international open access, published by Science Master Program of Science Education Graduate Program University of Mataram, contains scientific articles both in the form of research results and literature review that includes science, technology and teaching ...