<![CDATA[The principle of sperm preservation is to prolong the viability of spermatozoa by reducing the degree of metabolism during storage at low temperatures, and one method involved was a cryoprotectant as a sperm protector. This study aimed to determine the best type of cryoprotectant for refrigeration storage of goldfish Carrasius auratus sperm. A completely randomized design was used, and five types of cryoprotectant were tested, namely Methanol, Ethanol, Ethylene Glycol, Glycerol, and DMSO at a 10% concentration combined with 15% egg yolks. The sperm was diluted with Ringer’s solution and stored at 4⁰C for 48 hours. The ANOVA test revealed that cryoprotectant significantly affected the motility and viability of sperm after 48 hours of refrigeration (P0.05). The results showed that 10% DMSO combined with 15% egg yolk could maintain sperm motility and viability better than other treatments, with values of 85.80% and 86.50%, respectively, which were significantly different from the other treatments. Glycerol produced the second-best sperm quality with motility and viability values of 71.13% and 74.13%, respectively. Therefore, DMSO is the most effective cryoprotectant for refrigeration storage of goldfish sperm.]]>
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