<![CDATA[This research is motivated by the importance of STEM knowledge for pre-service science and mathematics teachers in the 21st century. This research aims to analyze the STEM knowledge of pre-service science and mathematics teachers. The research method was carried out by distributing questionnaires with STEM knowledge indicators to the sample. The sample in this research was 86. They were 59 pre-service science teachers and 27 pre-service mathematics teachers, with different years of education, namely the 1st, 2nd, 3rd, and 4th year of Universitas Negeri Semarang. The results of this research show that the average understanding of pre-service science and mathematics teachers regarding to the STEM-based learning in the definition aspect is 4.11, objectives are 3.94, benefits are 3.94, aspects are 3.69, components are 3.71, characteristics is 3.75, and implementation is 3.86. This proves that pre-service science and mathematics teachers' understanding of the definition of STEM-based learning is very good, while pre-service science and mathematics teachers' understanding of the objectives, benefits, aspects, components, characteristics, and implementation of STEM-based learning is good.]]>
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