Journal of Fisheries & Marine
Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Pengaruh Induksi Bakteri Pseudomonas aeruginosa Terhadap Human Umbilical Vein Endothelial Cells (HUVECS) Culture [ The Influence Of Pseudomonas aeruginosa Induction To The Human Umbilical Vein Endothelial Cells (HUVECS) Culture ]

Dwi Yuni Nur Hidayati (Departemen Mikrobiologi Klinik, Fakultas Kedokteran,Universitas Brawijaya, Indonesia)

Article Info

Publish Date
01 Apr 2009

Abstract

Abstract One of the cause of negative gram bacteria is Pseudomonas aeruginosa (Ps. aeruginosa). It continuing become sepsis on the first step of patogenesa happen sticking between aeruginosa with endhotel cell. This sticking is mediated by adhesi molecul which have characterize same with hemaglutin protein. The main study of this research is to know the profile of endothelial cell culture within induction of Ps. aeruginosa. The methods are Ps. aeruginosa (9064) which have been isolated then processes on TCBS Media and continued with isolation terracely. The profile of weight molecule protein obtained by SDS PAGE and then conducted electroelution. The test of hemaglutinin using eritrosit mencit. The receptor cell used is endhotel cell (HUVECs) culture. The result show that dosage of hemaglutin protein of Aeruginosa bring effect to profile of HUVECs culture determined by index adhesion of Ps. aeruginosa to HUVECs culture. We conclude that protein 38,19 kDa give effect to profile of HUVECs culture in vitro.

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Journal Info
Journal of Fisheries & Marine
Website

Abbrev

JIPK

Publisher

Universitas Airlangga

Subject

Biochemistry, Genetics & Molecular Biology

Description

Jurnal Ilmiah Perikanan dan Kelautan (JIPK; English: Scientific Journal of Fisheries and Marine) ISSN International Centre | ISSN:2528-0759 (Online) | ISSN: 2085-5842 (Print) JIPK is a peer-reviewed and open access biannually (April and November) that published by the Faculty of Fisheries and ...