INDONESIAN JOURNAL OF PHARMACY
Vol 25 No 2, 2014

IN VITRO EFFECT OF Chloroprocta SP. MAGGOTS SECRETION ON Staphylococcus epidermidis BIOFILM AND THE EXPRESSION LEVEL icaA OF GENE

Anjarwati, Dwi Utami (Lab. of Microbiology , Faculty Medicine and Health Science. Jendral Soedirman University. Jl. Dr. Suparno-Purwokerto, 53122)
Setiawati, Setiawati (Lab. of Farmacology Faculty Medicine and Health Science of Jendral Soedirman University. Jl. Dr. SuparnoPurwokerto, 53122)
Mujahidah, Mujahidah (Dept. of Microbiology Faculty of Medicine of Diponegoro University – Kariadi Hospital. Jl. Doktor Soetomo No.14, 50244, Indonesia)
Hapsari, Rebriarina (Dept. of Microbiology Faculty of Medicine of Diponegoro University – Kariadi Hospital. Jl. Doktor Soetomo No.14, 50244, Indonesia)
Nuryastuti, Titik (Dept. of Microbiology Faculty Medicine. Gadjah Mada University. Yogyakarta, Sekip Utara 55281, Indonesia)



Article Info

Publish Date
01 Apr 2014

Abstract

Biofilm formation and the expression of icaA gene can be induced by environment conditions that are potentially toxic for bacterial cells. The effect of green flies maggots secretion to biofilm was studied some years ago to investigate in vitro effect of secretion of Chloroprocta sp. maggots on the formation of Staphylococcus epidermidis biofilm (phenotype) and the expression level of icaA gene (genotype) for indicating its mechanism on bacterial biofilm eradication. Microtiter plate biofilm assay was used to measure the effect of Chloroprocta sp. maggots secretion at various concentration on S. epidermidis biofilm. The expression level of icaA gene was performed by Real TimePCR using lightcycler method. The biofilm susceptibility test was done against maggots excretion/secretion  using MTT assay. Whereas planktonic  susceptibility testing was carried out  using Kirby Bauer method. In the presence of maggots secretion at low concentration (5%), biofilm formation of S. epidermidis 734 was induced. In contrast, the expression level of icaA gene in  production of maggots excretion/ secretion at concentration of 5% was lower than that of without maggots secretion (1/2 Fold). Eradication of bacterial biofilm was demonstrated after 48h incubation (MD=-0,011;P<0,05), but planktonic cell. In vitro difference effect of the Chloroprocta sp. maggots secretion at low concentration to phenotype and genotype of S. epidermidis biofilm showed that the possibility of maggots secretion ability to eradicate bacterial biofilm was not mainly due to the expression level of icaA gene.

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Journal Info

Abbrev

3

Publisher

Subject

Medicine & Pharmacology

Description

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas ...