<![CDATA[Theobroma cacao L. is a plant that is reported to have strong antioxidant potential. A study was carried out to determine the antioxidant activity of dichloromethane extract of cocoa pod husk using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The cocoa pod husk was macerated using n-hexane, ammonia-dichloromethane, and methanol. The TCD extract of cocoa pod husk showed the lowest IC50 value of 14.59 ± 0.54 ppm based on the DPPH inhibition assay. The dichloromethane extract was isolated by gravity column chromatography with Silica gel G60 as the stationary phase and n-hexane: ethyl acetate as the mobile phase (gradient elution system). Fractions with identical staining patterns were combined into six fractions (TCD 1-TCD 6). The TCD 6 showed the lowest IC50 value of 127.05 ± 0.54 ppm according to the DPPH inhibition assay. Based on the TLC results, TCD 4 was further isolated and four fractions (TCD 4.1 to TCD 4.4) were obtained. The TCD 4.4 had the lowest IC50 value (38.66 ± 0.96 ppm). The TCD extract showed the presence of the dominant compound, γ-sitosterol, as determined by GC-MS analysis.]]>
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