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PENGARUH MASSA RAGI SACCHAROMYCES CEREVISIAE TERHADAP KADAR BIOETANOL BERBAHAN DASAR LIMBAH KULIT KOPI ARABICA (COFFEA ARABICA L.) Febrina, Resa Vernia; Nasution, Reni Silvia
AMINA Vol 2 No 1 (2020): April 2020
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/amina.v2i1.498

Abstract

Arabica coffee skin waste contained 49% cellulose, 24,5% hemicellulose, and 7,63% lignin that can be used to produce bioethanol. The purpose was to know the effect of the various mass of yeast Saccharomyces cerevisiae (3, 9, and 15 gram). The method used consists of several steps such as pretreatment, hydrolysis, fermentation using Saccharomyces cerevisiae, distillation process, and determining bioethanol content using chromatography gas. The bioethanol content from 25 gram arabica coffee skin waste with 3, 9, and 15 gram yeast for 3 days fermentation was 0,35%; 0,57%; and 1,46%. The highest bioethanol content was 1,46% found by adding 15 gram yeast Saccharomyces cerevisiae for 3 days fermentation.
Antioxidant ativity of dichloromethane extract from cocoa pod husk (Theobroma cacao L.) FEBRINA, RESA VERNIA; YAHYA, MUSTANIR; GINTING, BINAWATI; SAIDI, NURDIN
Jurnal Natural Volume 24 Number 2, June 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v24i2.36174

Abstract

Theobroma cacao L. is a plant that is reported to have strong antioxidant potential. A study was carried out to determine the antioxidant activity of dichloromethane extract of cocoa pod husk using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The cocoa pod husk was macerated using n-hexane, ammonia-dichloromethane, and methanol. The TCD extract of cocoa pod husk showed the lowest IC50 value of 14.59 ± 0.54 ppm based on the DPPH inhibition assay. The dichloromethane extract was isolated by gravity column chromatography with Silica gel G60 as the stationary phase and n-hexane: ethyl acetate as the mobile phase (gradient elution system). Fractions with identical staining patterns were combined into six fractions (TCD 1-TCD 6). The TCD 6 showed the lowest IC50 value of 127.05 ± 0.54 ppm according to the DPPH inhibition assay. Based on the TLC results, TCD 4 was further isolated and four fractions (TCD 4.1 to TCD 4.4) were obtained. The TCD 4.4 had the lowest IC50 value (38.66 ± 0.96 ppm). The TCD extract showed the presence of the dominant compound, γ-sitosterol, as determined by GC-MS analysis.