Jurnal Bahan Alam Terbarukan
Vol 5, No 2 (2016): December 2016 [Nationally Accredited]

KINERJA ENZIM GANDA PADA PRETREATMENT MIKROALGA UNTUK PRODUKSI BIOETANOL

Padil, Padil ( 1Jurusan Teknik Kimia, Fakultas Teknik Universitas Riau Kampus Bina Widya Jl. HR Subrantas Km 12,5 Simpang Panam, Pekanbaru Jurusan Teknik Kimia, Fakultas Tekinik Universitas Gadjah Mada Jl Grafika No 2, Sinduadi, Mlati, Kec. Sleman, Daerah Istimew)
Syamsiah, Siti ( Jurusan Teknik Kimia, Fakultas Tekinik Universitas Gadjah Mada Jl Grafika No 2, Sinduadi, Mlati, Kec. Sleman, Daerah Istimewa Yogyakarta)
Hidayat, Muslikhin ( Jurusan Teknik Kimia, Fakultas Tekinik Universitas Gadjah Mada Jl Grafika No 2, Sinduadi, Mlati, Kec. Sleman, Daerah Istimewa Yogyakarta)
Kasiamdari, Rina Sri ( Laboratorium Sistematika Tumbuhan, Fakultas Biologi Universitas Gadjah Mada Jl. Teknika Selatan, Sekip utara, Daerah Istimewa Yogyakarta)



Article Info

Publish Date
30 Jan 2017

Abstract

The use of biomass of microalgae as a feedstock to produce bioethanol is very promising, it is caused by a large amount of carbohydrates contained in microalgae physiology cell. The main obstacle of enzymatic hydrolysis in order to produce bioethanol is the bound starch granules in a rigid cell wall. Therefore, pre-treatment steps needed to remove and convert complex carbohydrates into simple sugars before the fermentation process. Tetraselmis Chuii microalgae species are green microalgae (Chlorophyta) in which the cell wall containing cellulose and hemicellulose as the main constituent, therefore, this study observe the effect of the use of cellulase enzymes and xylanase as a strategy to open up the cell walls of microalgae. Another investigated parameter is the enzyme concentration, temperature, pH, and methods of use of enzymes. The results showed that the highest yield of glucose obtained was 31.912% (w / w) and is achieved under the conditions of a temperature of 45oC, pH of 4.5, the amount of biomass of microalgae as 5 g/L, the concentration of cellulase enzymes and xilanase 30% (w / w) at 40 minute at mechanism using cellulase and xylanase enzymes simultaneously.

Copyrights © 2016






Journal Info

Abbrev

jbat

Publisher

Subject

Materials Science & Nanotechnology

Description

This journal presents articles and information on research, development and applications in biomass conversion processes (thermo-chemical conversion; physico-chemical conversion and bio-chemical conversion) and equipment to produce fuels, power, heat, and value-added chemicals from biomass. A ...