Jurnal Sumberdaya Akuatik Indopasifik
Vol 1 No 2 (2017): November

Identifikasi Ikan Genus Mystus Dengan Pendekatan Genetik

Taufik Budhi Pramono (PS Budidaya Perairan, FPIK, Unsoed, Purwokerto, 53123, Indonesia)
Diana Arfiati Arfiati (Program Pasca Sarjana, FPIK, UB, Malang, 65145, Indonesia)
Maheno Sri Widodo (Program Pasca Sarjana, FPIK, UB, Malang, 65145, Indonesia)
Uun Yanuhar Yanuhar (Program Pasca Sarjana, FPIK, UB, Malang, 65145, Indonesia)



Article Info

Publish Date
03 Dec 2017

Abstract

The fish of the Bagridae Family in Indonesia reach until 60 species and one of them is from the genus Msytus. The method is used for fast and accurate species identification was needed. DNA barcoding was a new identification method with molecularly approach. The CO1 gene was amplified and PCR products were sequenced and analyzed using bioinformatics software. Editing of sequencing results and determining the nucleotide composition were analyzed with Mega5 software. The DNA sequence was aligned with ClustalW vers. 1.4. Sequences are compared with GenBank data using BLAST (Basic Local Aligment Search Tools) and BOLDSystems. Phylogenetic tree was made using Neighbor_Joining method. One example of the identification of fish from the confirmed Mystus genus is Mystus nigriceps being the Mystus singaringan.

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Journal Info

Abbrev

JSAI

Publisher

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science

Description

Journal name: Jurnal Sumberdaya Akuatik Indopasifik. Focus and scope of Jurnal Sumberdaya Aquatik Indopasifik are research paper in term of fisheries and marine science which can be seen also in some of study aspect such as the conservation, management, aquaculture, enginering, biology, chemistry, ...