Jurnal Perlindungan Tanaman Indonesia
Vol 16, No 2 (2010)

Pengaruh Tinopal terhadap Patogenisitas Nucleopolyhedrovirus pada Spodoptera litura

Ma'unah Ambarwati (Program Pascasarjana Fakultas Pertanian Universitas Gadjah Mada)
Arman Wijonarko (Fakultas Pertanian Universitas Gadjah Mada)
Sedyo Hartono (Fakultas Pertanian Universitas Gadjah Mada)



Article Info

Publish Date
21 Dec 2010

Abstract

Susceptibility of the armyworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) to nucleopolyhedrovirus was evaluated using droplet feeding methods. S. litura was originally collected from the field in Bantul and has been reared continuously in the laboratory using artificial diet. The tested instars were exposed a series concentration of nucleopolyhedrovirus (2×103, 2×104, 2×105, 2×106, 2×107, 2×108, 2×109 PIB/ml) which were added with Tinopal 0,5% and 1%. The result indicated that the larval mortality of 3rd, 4th, and 5th instars Tinopal, significantly different with the addition of 1% Tinopal. This addition increased the effectiveness of NPV for 235, 25117, and 6.6 million fold. The observation of the larval midgut which was treated by Tinopal, showed that Tinopal physically disrupt the peritrophic membrane. Therefore, it can be suggested that the Tinopal facilitates the entry of NPV to the host insect. 

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