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All Journal Jurnal Fitopatologi Indonesia Jurnal Ilmu Pertanian Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri JURNAL AGROTEKNOLOGI AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan jurnal Ilmu Pertanian (Agricultural Science) Jurnal Penelitian Tanaman Industri (Littri) Agrokompleks

SEDYO HARTONO

Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281

Author-ID : 3094341

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Incidence of Soybean Mosaic Disease in East Java Province Andayani, Wuye Ria; Sumardiyono, Y. B.; Hartono, Sedyo; Yudono, Prapto
AGRIVITA, Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

The objectives of this study were: 1) to identify the mosaic symptom severity and the incidence the virus and relate these to soybean yield reduction is four regions of East Java; 2) diagnostic of the symptom using Indirect ELISA, RT-PCR and electron microscope observation. Results from experiments indicated that soybean plants infected with SMV and CMMV, alone or in combination produced mosaic symptom. Incidence of the virus, as judged by symptomatology, ranged from mild to severe infection the percentage of plants being from 13.42-30.10%. Soybean plants with mosaic symptom caused SMV from an early stage of development (14-28 days after planting). Soybean mosaic virus belongs to the virus family Potyviridae. Specific DNA fragment of 1687 bp was successfully amplified from soybean infected by SMV isolate Ngawi, Madiun, Magetan, and Ponorogo. Specific DNA fragment of 1385 bp was successfully amplified from SMV by CI coding region. The mosaic symptom on soybean plant (28-42 days after planting) caused CMMV. Flexious virus particle 650 nm in length was observed on electron microscope. It caused local lesions on Chenopodium amaranticolor, but not detected by I ELISA with antiserum SMV and RT-PCR with universal primer. Keywords: SMV, CMMV, I.ELISA, RT-PCR.

Preferensi Wereng Batang Cokelat terhadap Varietas Padi dan Ketahanan Varietas Padi terhadap Virus Kerdil Hampa Suprihanto, Suprihanto; Somowiyarjo, Susamto; Hartono, Sedyo; Trisyono, Y. Andi
Jurnal Penelitian Pertanian Tanaman Pangan Vol 35, No 1 (2016): April 2016
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Rice ragged stunt virus (RRSV) is transmitted by brown planthopper in the persistent manner. This disease in recent years has become a serious problem in Indonesia and some other countries, such as China, Vietnam, Philippines, and Thailand. Disease control is usually conducted by the vector control using insecticides, so often causes an environmental pollution. An alternative control method is using the environmentally friendly of resistant varieties. The purpose of this study was to determine the level of preference of brown planthopper (BPH) to some varieties and rice germplasms, the resistance of several varieties against rice ragged stunt virus (RRSV) disease and its effectiveness as an inoculum source of virus (RRSV). A total of 15 varieties of rice were used in preference test of BPH and resistance test to RRSV. The test for resistance varieties to RRSV was conducted by transmission of 2nd instar of BPH for 3 days of acquisition feeding period, 10 days incubation period and 24 hours inoculation period with population density of 3 BPH/plant. Disease index was calculated and used to determine the level of plant resistance. Varieties that showed resistant, moderately resistant and susceptible responses were selected and were used as a source of inoculum to be transmitted on to TN1 variety susceptible check variety to know the effectiveness of varieties as source of virus inoculum. The results showed that of the 15 varieties tested, Situ Bagendit, Utri Merah, Mentik Wangi, Mahsuri, and Inpari 1 each was less favored by BPH to settle and to multiply. Mentik Wangi, Tetep, Utri Merah, and Swarnalata each showed resistant response to RRSV. Transmission test to susceptible variety (TN1) showed that the variety of Situ Bagendit, Inpari 13, Mentik Wangi, and Tetep each has a fairly low effectiveness as a source of inoculum as indicated by the lower percentage of infection and disease index on the transmited test plants.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i1.34

DETECTION AND TRANSMISSION OF RICE STUNT VIRUS ON CIHERANG AND SITU BAGENDIT VARIETIES Helina, Selvi; Sulandari, Sri; Hartono, Sedyo; Trisyono, Andi
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 2 (2018): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Detection and Transmission of rice stunt virus on Ciherang and Situ Bagendit Varieties. The explosion of brown planthoppers recently has caused reduction of rice production in Indonesia. Brown planthoppers do not only act as pest, but also transmit Rice grassy stunt virus (RGSV) and Rice ragged stunt virus (RRSV). Detection of the existence of the two viruses in rice plants and vector insects is important to be done to ensure that the virus is infected with the vector. The aim of this research is to detect the existence of virus in varieties of Ciherang and Situ Bagendit as a result of transmission in the laboratory and to find out the ability of brown planthoppers to transmit stunt virus to both of the varieties. This research was compiled using Completely Randomized Design (CRD) with 4 treatments, namely healthy rice plants of Ciherang and Situ Bagendit varieties, Ciherang and Situ Bagendit varieties which were infested by brown planthoppers each with 5 repetitions. The parameters observed were incubation period, symptoms, plant height, number of leaves and incidence of disease. The data on plant height, number of leaves and incidence of disease were analyzed using ANOVA and continued with the Least Significant Difference (LSD) test at the level of 5%. The results showed that Ciherang and Situ Bagendit varieties were only positively infected by Rice ragged stunt virus. The results of the rice transmission showed that Ciherang variety had a faster incubation period of 10 DAI while Situ Bagendit was 14 DAI, but the two varieties showed an inhibition of growth in plant height and number of leaves compared to healthy plants with each incidence of 51.3% and 46.3%.

Keragaman Virulensi dan Konstruksi Molekuler Virus Tungro pada Padi dari Daerah Endemis Praptana, R. Heru; Sumardiyono, Y. B.; Hartono, Sedyo; Trisyono, Y. Andi; Widiarta, I Nyoman
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Tungro is an important disease of rice, constraining to the rice production in Indonesia. Tungro is caused by the infection of two different viruses namely tungro bacilliform virus (RTBV) and tungro spherical virus (RTSV). Both viruses are only transmitted by green leafhoppers, especially Nephotettix virescens in a semipersistent manner. The variation of tungro viruses from different areas had been reported, and there is a specific relationship between resistance variety and tungro virus isolate. It is important therefore, to study the virulences and the genetic diversities of tungro viruses derived from the endemic areas in Indonesia. This study was aimed to identify the virulence and the molecular diversity of tungro viruses from endemic areas in Indonesia. Susceptible variety TN1 was used in the study. Surveys and collection of the infected plants and green leafhoppers were conducted in some tungro endemic areas, including: West Java, Central Java, Yogyakarta, Central Sulawesi, West Sulawesi, South Sulawesi, Bali and West Nusa Tenggara. Artificial virus transmission using test tube method was used in the virulence test. Green leafhoppers caught from the field were used as vector transmitters. The virulence of tungro viruses was determined based on diseases indexes (DI). Results showed that the virulence of tungro viruses varied among region in the endemic areas in Indonesia. The Central Java virus isolate was the most virulence; however, not all isolates from endemic areas in the island of Java were more virulent than those from outside of Java. The presence of RTBV and RTSV was detected in the infected TN1 plants. The existences of molecular diversities of tungro viruses from the endemic areas were observed. The relationship between combination of DNA bands of RTBV and RTSV with the virulence in endemic areas outside of Java was more complex than it was in West Java and Central Java. The molecular diversities of tungro viruses were not correlated with the geographic difference of the endemic areas, nor with the virulences.

DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD Astuti, Suryani Titi; Sulandari, Sri; Hartono, Sedyo; Somowiyarjo, Susamto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12156-62

Detection and identification of yellow mosaic stunt disease on Petunia sp. using nested PCR method. Yellow mosaic stuntdisease was found at some nurseries of Petunia in Sleman, Yogyakarta, also in Muntilan and Magelang Central Java. Thedisease was very important due to its ability reducing the quality and quantity of Petunia seedlings. The causal agent of thedisease may be carried over to imported seeds and necessary to identify as a basic information for developing controlstrategies. This research was done by mechanical transmission on indicator plants. The observation of the causal agents wasconducted using electron microscope with quick dipping method and the molecular detection was done using nested PCRwith TobRT up1-TobRT do2 as the external primers and TobN up3-TobN do4 as the internal primers. Mechanical inoculationshowed chlorosis symptoms that developed into local spot on Chenopodium amaranticolor as well as mosaic and veinbanding on Nicotiana benthamiana. The observation using electron microscope showed rod-shaped virus particles sizedapproximately 300 nm and by PCR method produced around 568 bp and 400 bp DNA band. Based on the sequence analysis,the disease was caused by Rehmania mosaic virus. This type of Tobamovirus has 96% similarity with ReMV-Japan. ReMV, aplant pathogen which was a member of Tobamovirus that has never been reported in Indonesia. This research was the firstreport of ReMV in Indonesia infecting Petunia as ornamental plant.

Laporan Pertama tentang Infeksi Polerovirus pada Tanaman Cabai di Daerah Bali, Indonesia Gede Suastika; Sedyo Hartono; I Dewa Nyoman Nyana; Tomohide Natsuaki
Jurnal Fitopatologi Indonesia Vol 8 No 5 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

During a visit to Bali Island in September 2011, we found chilipepper plants exhibiting yellowing symptoms. The infected plants showed quite similar symptom to those of the recently reported Pepper yellow leaf curl virus from Israel and Japan, but there was no shortening of internodes or leaf rolling. We have ampliﬁed part of the genome of a virus associated with this disease using degenerate primers for members of the genus Polerovirus. The reverse transcription-polymerase chain reaction (RT-PCR) from symptom-bearing samples resulted in the amplification of a 650 bp band which is the expected size. RT-PCR from healthy samples did not produce an amplicon. Based on our results, we concluded that yellowing disease on chilipepper observed in Payangan, Gianyar, Bali is associated with a virus belonging to the genus Polerovirus. This is the first report on Polerovirus infection in chilipepper in Indonesia. Some researches for elucidation of the virus species and biological/molecular characters are being conducted.Key word: chilipepper, Indonesia, Polerovirus

Pengendalian Penyakit Budok dengan Fungisida dan Deteksi Residu pada Daun Nilam Christanti Sumardiyono; Sedyo Hartono; Nasrun Nasrun; Sukamto Sukamto
Jurnal Fitopatologi Indonesia Vol 9 No 3 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Budok caused by Synchytrium pogostemonis is the important and destructive disease on patchouli plant (Pogostemon cablin). Chemical control must be done in incidence with high disease intensity and prediction of outbreak. The experiment was done using benomyl (Benlate 50 WP) and cuprous oxide (Kocide 77WP) 0.1%, 0.2% and mixture of both ( 1 g cuprous oxide and 1 g L-1 benomyl). Patchouli plant was sprayed eight times with one week interval. Harvesting was done two weeks after the end of spraying. Residue analysis of Cu was done with AAS and benomyl residue with HPLC. The result showed that 0.1% benomyl significantly reduced the disease intensity. The other treatments showed lower result. Harvested patchouli plants treated with 0.1% benomyl showed higher yield compare with 0.2% benomyl and cuprous oxyde. Cu residue in dried leaves was between 460 ppm and 950 ppm. Residue of benomyl was 54–100 ppb. Diseased seedling still can be used after spraying by benomyl four times with 2 weeks interval.

Identifikasi Molekuler Tobacco mosaic virus pada Anggrek di Sleman, Yogyakarta Soesamto Somowiyarjo; Sedyo Hartono; Sri Sulandari; Sekar Utami Putri
Jurnal Fitopatologi Indonesia Vol 12 No 2 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Tobamovirus is a group of virus with a wide host range, including orchid plant which considered as an economically important plant. This research aimed to identify Tobamovirus infecting orchids. Virus isolates were collected from orchid nursery in Sleman, Yogyakarta. Plant extract from orchid showing necrotic flex symptom was inoculated to indicator plants Chenopodium amaranticolor. Chlorotic local lesion symptoms occurred within 3 days after inoculation. RNA total from symptomatic C. amaranticolor was extracted by using a commercial kit. cDNA was synthesized using oligo d(T) primer. Amplification of cDNA using partial movement protein specific primers TMV-1F and TMV-2R was successfully amplified the amplicon with size ± 422 bp. The nucleotide sequences of this amplicon showed highest DNA homology (98%) with Tobacco mosaic virus Yongren-2 isolat from China.

Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa Umi Kulsum; Sedyo Hartono; Sri Sulandari; Susamto Sumowiyarjo
Jurnal Fitopatologi Indonesia Vol 12 No 6 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Soybean (Glycine max) is an important food crops in Indonesia, but the production is considered low. Infection of Cowpea mild mottle virus (CPMMV) may affect to the productivity of the crops. The virus cause a variety of symptoms on soybean such as mosaic, chlorosis, necrosis, distortion, and leaf dwarf. The aims of this study were to detect and identify CPMMV by molecular technique from some varieties of soybeans, i.e. ‘Anjasmoro’, ‘Argomulyo’, ‘Grobogan’, and two local Edamame varieties. Symptomatic samples was obtained randomly from main soybean growing areas in Java. DNA amplification using specific primer for coat protein of CPMMV was only successfully gained from leaf samples of var. Argomulyo from Malang which showing mosaic on the leaf surface, vein clearing, and chlorosis. Further amino acid and nucleotide sequence analysis showed that this CPMMV isolate has highest homology (99%) to CPMMV isolate of French bean from China.

Co-Authors Alvina Clara Giovanni Aminatun Munawarti Anak Agung Gde Raka Swastika Andi Khaeruni Ani Widiastuti Argawi Kandito Argawi Kandito Argawi Kandito Arman Wijonarko Asmar Hasan Astuti, Suryani Titi Azizah Ridha Ulilalbab Budi Setiadi Daryono Cahyo Hertanto Christanti Sumardiyono Christina Retna Handayani Deden Sukmadjaja Dewi Rahmitasari Didit Setiyawan Dini Wahyu Kartika Sari Efendi, Darda Emerensiana - Uge Emerensiana Uge Erna Anastasia Esti Prasetya Ningrum Fariha Wilisiani Fitri Kusumaningrum Gede Suastika Gusnawaty HS GUSNAWATY HS, GUSNAWATY Hasdiana Hasdiana Helina, Selvi Heri Widarta I Dewa Nyoman Nyana I Nyoman Widiarta Ika Roostika Ika Roostika Ismiyatuningsih Ismiyatuningsih Jun Kobayashi La Ode Santiaji Bande Ma'unah Ambarwati Mery Windarningsih Muhammad Botek Muhammad Muhsin MUHAMMAD TAUFIK Muhammad Taufik Mustika Ajeng Kartini Putri Pertiwi Nanda Kusumandari Nasrun Nasrun NOOR AIDAWATI Nur Isnaini Ulfa Nuri Yusmarlita Nurjanah Nurjanah Praptana, R. Heru Prapto Yudono Prapto Yudono PURNAMA HIDAYAT R. Heru Praptana Rahayu Mallarangeng Rahma Ayu Priani Resti Fajarfika Retno Mastuti Saipul Abbas Sekar Utami Putri Selvi Helina Serafinah Indriani Siti Anima Hisein Siwi Indarti Soesamto Somowiyarjo Somowiyarjo, Susamto Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari SRI SULANDARI Sri Sulandari Sri Sulandari Sri Sulandari Subandi Sukamto Sukamto Sumardiyono, Y. B. Suprihanto, Suprihanto susamto - somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Sumowiyarjo Syair Syair Tomohide Natsuaki Tri Harjaka Tri Joko Tri Joko Tri Maruto Aji Tri Maruto Aji Tri Retno Widyastuti Triharso Triharso Trisnani Alif Triwidodo Arwiyanto Tuty Arisuryanti Umi Kulsum Widiarta, I Nyoman Wiwik Endarsih Wuye Ria Andayani Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. B. Sumardiyono Y. B. Sumardiyono Y.M.S. Maryudani Yashanti B. Paradisa Yashanti Berlinda Paradisa YB Sumardiyono

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