Indonesian Journal of Biotechnology
Vol 10, No 1 (2005)

Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate

Erma Sulistyaningsih (Faculty of Medicine, Jember University, Jember 68121, Indonesia)
Sukarti Moeljopawiro (Faculty of Biology, Gadjah Mada University, Yogyakarta 55281, Indonesia)
Jarot Subandono (Faculty of Medicine, Sebelas Maret State University, Solo, Indonesia)
Wayan T. Artama (Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta 55281, Indonesia)

Article Info

Publish Date
13 Jun 2005

Abstract

Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the research was to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinant technology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and it was used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate was amplified and cloned in a prokaryote cloning vector. The recombinant GRA1-encoding gene was then digesting using EcoRI restriction endonuclease and sequencing. The result showed that the recombinant GRA1- encoding gene consisted of DNA sequences encoding all signal peptide and mature peptide of GRA1 protein. Alignment of recombinant GRA1 sequence to gene encoding GRA1 protein of Toxoplasma gondii RH isolate showed 100% homologous.

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Journal Info
Indonesian Journal of Biotechnology
Website

Abbrev

ijbiotech

Publisher

Universitas Gadjah Mada

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...