Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Vol 11, No 3 (2016): December 2016

Purification and Characterization of Transglutaminase from Local Streptomyces Sp. TTA 02 SDS 14

Lia Siti Nur'amaliyah (Bogor Agricultural University)
Dewi Seswita Zilda (RCMFPPB)
Nisa Rachmania Mubarik (Bogor Agricultural University)

Article Info

Publish Date
27 Dec 2016

Abstract

Streptomyces sp. TTA 02 SDS 14 is a transglutaminase producing bacteria which previously had been  screened along with more than one hundred isolates. This research aimed to purify and characterize transglutaminase from this strain. Transglutaminase was purified from crude enzyme by ultrafiltration, Q-Sepharose ion exchange chromatography and Sepacryl S200 size exclusion chromatography sequentially, obtaining yield and purification fold of  1.36%  and 27 folds, respectively. The molecular weight of the purified transglutaminase was 72 kDa detected by zymogram gel electrophoresis. The optimum temperature and pH were 50°C and 6. The transglutaminase was stable at 45°C and could be activated in the presence of 5 mM and 10 mM of Na+, K+, Li+,Ca2+, Mg2+, BPB (4-bromo-phenacyl bromide), and IAA (iodo acetamide acid), but the activity was inhibited by  the presence of Cu+, Zn2+, and PMSF (phenyl methyl sulfonyl fluoride).

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Journal Info
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Website

Abbrev

squalen

Publisher

Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Description

Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be ...