<![CDATA[Objective: To obtain the effective method of ovarian function preservation
with granulose cell apoptosis assessment. Ovarian tissue
vitrification became a method for ovarian function preservation in
women with cancer. This technique can be done anytime without
delay on cancer therapy both in prepubertal and unmarried patient.
It can also store many primordial follicles. Ovarian tissue vitrification
study is still limited to animal test and there are no data about apoptosis
assessment after ovarian vitrification in human ovary.
Method: This quasi experimental study was held in Department of
Obstetrics and Gynecology Faculty of Medicine University of Indonesia
- Dr. Cipto Mangunkusumo General Hospital and Fatmawati
Hospital Jakarta from March 2012 to May 2015. Ovaries from thirteen
women between 31 and 37 years old who underwent oophorectomy
with gynecological indication were examined.
Result: There was no morphological difference between follicles
from fresh and warmed-vitrified ovaries. The mean protein Bax
expression on the fresh ovaries assessed in the form of H-score
was 1.66 (SD 0.14) compared with 1.68 (SD 0.13) on the warmedvitrified
group (p=0.165). The mean protein Bcl-2 expression on the
fresh ovaries examined in the form of H-score was 1.73 (SD 0.10)
compared with 1.71 (SD 0.10) on the warmed-vitrified group
(p=0.068).
Conclusion: Ovarian tissue vitrification does not affect the Bax and
Bcl-2 expression on human ovary.
[Indones J Obstet Gynecol 2016; 4-2: 88-92]
Keywords: apoptosis, bax, Bcl-2, ovarian tissue vitrification]]>
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