ASEAN Journal on Science and Technology for Development
Vol. 27 No. 1 (2010): ASEAN Journal on Science and Technology for Development (AJSTD)

R unning Head: Novel Recombinant Phytate - d egrading Enzyme Production Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology

Abd - ElAziem Farouk (Department of Biology, Faculty of Science, University Brunei Darussalam, Tungku Link BE1410, Brunei Darussalam Faculty Science,University of Taif, Al Hawya, P.Box. 8888, Kingdom of Saudia Arabia)
Anis Shobirin Meor Hussin (Faculty Science,University of Taif, Al Hawya, P.Box. 8888, Kingdom of Saudia Arabia. Department of Food Technology, Faculty of Science and Technology, University Putra Malaysia, 43400, UPM, Serdang, Selangor.)
Ralf Greiner (Max Rubner-Institute, Department of Food and Bio Process Engineering, Haid-und-Neu-Straße 5, 76131 Karlsruhe, Germany)
Shareef Mohideen Ismail (Faculty Science,University of Taif, Al Hawya, P.Box. 8888, Kingdom of Saudia Arabia.)
Mohamed Faizal Batcha (Faculty Science,University of Taif, Al Hawya, P.Box. 8888, Kingdom of Saudia Arabia.)
Hamadah Mohd Nur Lubis (Faculty Science,University of Taif, Al Hawya, P.Box. 8888, Kingdom of Saudia Arabia.)

Article Info

Publish Date
12 Nov 2017

Abstract

The production of phytase in a recombinant E.coliusing thepBAD expression system wasoptimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentrationin the cultivation media and the incubation temperature were optimized in order to maximize phytase productionusing 23 central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among thesefactors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature. OPtim al conditionsfor phytase production were determinedto be100 ?g/ml ampicilin, 0.2 % arabinose and an incubation temperature of37ºC.The production of phytase in the recombinant E. coliwas scaledup to 100ml and 1000ml.

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Journal Info
ASEAN Journal on Science and Technology for Development
Website

Abbrev

ajstd

Publisher

Universitas Gadjah Mada

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Mathematics

Description

The coverage is focused on, but not limited to, the main areas of activity of ASEAN COST, namely: Biotechnology, Non-Conventional Energy Research, Materials Science and Technology, Marine Sciences, Meteorology and Geophysics, Food Science and Technology, Microelectronics and Information Technology, ...