JURNAL ILMU KEFARMASIAN INDONESIA
Vol 9 No 1 (2011): JIFI

Pengkulturan Sampel Susu Fermentasi yang Mengandung Probiotik dan Identilikasinya dengan PCR

AMARILA MALIK (UNIVERSITAS INDONESIA)
INDRIANI INDRIANI (UNIVERSITAS INDONESIA)
LILIANA SARAGIH (UNIVERSITAS INDONESIA)
MAKSUM RADJI (UNIVERSITAS INDONESIA)



Article Info

Publish Date
30 Apr 2011

Abstract

Lactic Acid Bacteria (LAB) has long been used in fermented milk because its stability, unique taste and texture and also for its health benents that are provided by the probiotic. There are many fermented dairy products in the market nowadays that declared containing living culture or as probiotics. The aim of this study was to identify LAB from fermented milk product by Visual inspection on plate culture for their morphology, as well by molecular technique performing PCR and DNA sequencing methods. Morphology and growth incubation time of each isolate was observed. A pair of primer that is highly speciiic for LAB targeting the gene encoding for 168 rRNA, which represents sequences of region 677 to 693 of E. coli numbering has been used for PCR identiiication in this study. A total of nineteen isolates were obtained from 10 fermented milk samples that were grown on de Mann Rogosa Sharpe Agar (MRSA) media. Nine out of 19 isolates which represent each of the samples were selected. Genomic DNA extracted from those 9 isolates were used as template for PCR. The results showed that 8 amplicons were positively identitied as LAB, but only three identities indicated similar species as declared, while 5 others did not.

Copyrights © 2011






Journal Info

Abbrev

jifi

Publisher

Subject

Medicine & Pharmacology

Description

Jurnal Ilmu Kefarmasian Indonesia memuat tulisan ilmiah baik berupa naskah hasil penelitian maupun naskah konsep di bidang Kefarmasian. Diterbitkan setiap 2 (dua) kali dalam setahun yaitu pada bulan April dan ...