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Acta Biochimica Indonesiana
Published by Perhimpunan Biokimia dan Biologi Molekuler Indonesia
ISSN : 26546108     EISSN : 26543222     DOI : https://doi.org/10.32889
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Acta Biochimica Indonesiana (ActaBiolna) is a peer-reviewed and open-access journal that disseminates original research articles and review articles covering diverse topics in Biochemistry and Molecular Biology. The journal is published biannually by Indonesian Society for Biochemistry and Molecular Biology.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana" : 6 Documents clear
Palladium (II) chloride (PdCl2) spectrophotometry to determine lipoic acid concentration in plasma and leukocytes Novian Agni Yudhaswara; Ani Retno Prijanti; Mohamad Sadikin
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.29

Abstract

Background: Lipoic acid is a substance contained in intra and extracellular that act as a coenzyme of Pyruvate Dehydrogenase, also as an antidote, chelating agent and antioxidant. Measurement of lipoic acid is needed to determine the amount of lipoic acid that performs its functions either as a coenzyme or an antioxidant. Besides, this measurement requires a special tool such as High Performance Liquid Chromatography (HPLC) and a process that is available in rural or simple laboratories. Objective: A common and easy tool such as a spectrophotometer was conducted and could expected to be a tool of lipoic acid determination in body fluid such as plasma. Methods: Measurement of lipoic acid using spectrophotometry with UV methanol and visible PdCl2 has been tested and compared to HPLC measurement that was valid and reliable in drug measurement or pharmaceutical preparations. Results: Determination of lipoic acid in plasma and leukocytes using PdCl2 produced replicable, reliability and valid result, with high accuracy, precision and was not different from lipoic acid measurement using HPLC, p=0.99. While UV methanol was different compare to HPLC p =0.0001 or was not valid. Conclusion: The measurement of lipoic acid using PdCl2 visible method can be applied to determine the levels of lipoic acid (LA) and DHLA in plasma and equal to HPLC result.
Association of vitamin D level and vitamin D receptor A-1012G polymorphism with psoriasis – case control study Pocut Astari; Yahwardiah Siregar; Ariyati Yosi
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.47

Abstract

Background: Psoriasis is a chronic inflammation of the skin caused by combination of genetic, immune and environmental factors. The Vitamin D receptors alongside with plasma vitamin D (25(OH)D) level have known to be related with psoriasis. Vitamin D receptor polymorphism is one of the multiple polymorphisms that predispose individuals to several diseases. It is possible that this polymorphism is different among psoriatic patients and healthy one particularly in Medan, Indonesia population. Objective: We aimed to investigate associations between plasma level of 25(OH)D and one VDR gene polymorphism (A-1012G) with psoriasis. Methods: Fourty four psoriatic patients and 44 healthy control subjects’ DNA samples were obtained in this case control study and genotyped for A-1012G polymorphism by Polymerase Chain Reaction (PCR). The plasma vitamin D (25(OH)D) level of case and control subjects were examined using Enzyme Linked Immunosorbent Assay (ELISA). Results: Significant lower plasma 25(OH)D levels were found in control group (p<0.001) which consist of mostly young adult female. There is no significant relationship between AA, AG and GG genotype variances of A-1012G polymorphism with psoriasis (p=0.124). Conclusion: No significant association found between A-1012G polymorphism and psoriasis but there was a significant difference found between vitamin D level and psoriasis (p<0.001). From this study, vitamin D deficiency were more common in young adult female.
Comparison of the effectiveness of physical training and extract of soursop leaf to histopathology of abdominal aorta foam cells in hipercolesterolemia-diabetes rat Tika Hamidah Apriliana Nai; Retno Yulianti; Wahyunia Likhayati Septiana; Yuni Setyaningsih
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.48

Abstract

Background: Diabetes mellitus has become a public health problem globally. Type 2 diabetes mellitus, caused by lifestyle and high lipid diet, is the most common form. Hypercholesterolemia-diabetes is able to create endothelial injury that results in formation of atherosclerosis, starting with formation of foam cells. Objective: This research aimed to see the effectiveness of physical training and soursop (Annona muricata) leaves extract in lowering the amount of foam cells. Methods: This research was an experimental study which used the post test-only control group design with 35 Wistar strain rats which were randomly divided into 7 groups, each consisting of 5 rats. K1 was given standard diet, K2 was given atherogenic diet and alloxan, while K3, K4, K5, K6, and K7 were given atherogenic diet, alloxan, and different treatments based on designed group. K3 was given simvastatin, K4 was given metformin, K5 was given physical training, K6 was given soursop leaves extract (SLE), and K7 was given both physical training and SLE. The rats were euthanized after three weeks and aorta was taken to be made into histology slides for foam cells observation. Results: The result of Kruskal-Wallis and Mann-Whitney Post-Hoc Test showed significant difference (p<0,05) between K2 and K5, K6, K7 while it no significant difference (p>0,05) between K3, K4 and K5, K6, K7. Conclusion: Physical training, SLE, and the combination of both have a role in lowering formation of foam cells in atherosclerosis.
The effect of cyclophosphamide combined with zinc on male mice Mus musculus spermatozoa Muhammad Fadzrul Adhiwirawan; Yudhi Nugraha; Cut Fauziah
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.50

Abstract

Background: The usage of cyclophosphamide leads to infertility of reproductive system caused by acrolein. Acrolein can itself cause oxidative damage by depletion of cellular glutathione (GSH) by conjugation, leading to membrane disruption, DNA and mitochondrial damage and can exacerbate apoptosis, which may affect spermatogenesis. Zinc (Zn) which is constituents of superoxide dismutase, has a protective effect towards free radicals from physiological or pathologic effects to minimize the cell’s damage. Objective: The purpose of this research was to know the effect of Zn on the spermatozoa count of Mus musculus that was given cyclophosphamide intraperitoneally. Methods: In the present study, cyclophosphamide was administered in saline 200 mg/kg 1x weekly for 5 weeks by ip route, whereas Zn was supplemented by oral route with doses of 25, 50, 100 mg/Kg/day for 5 weeks. The data were analyzed with Anova and followed by Bonferroni Test at a significant level of 5%. Results: The result of this research revealed that high Zn diet and Cp administration decrease sperm count simultaneously. It showed by the decrease of sperm count from 1490 (1 x 103)/ml sperm in the control group becomes 240 (1 x 103)/ml sperm in treatment group with 100 mg/kg of oral Zn and 200 mg/kg of cyclophosphamide. Conclusion: This research shows that Zn supplement to prevent cyclophosphamide toxic effect in spermatogenesis does not have a protective effect, in fact its reduce sperm count by excess of Methallothine production and alter the spermatogenesis by reduce Cu intake from intestine.
Cytoglobin expression in rat kidney during exposure to systemic chronic hypoxia Ika Superti Daruningrum; Ani Retno Prijanti; Ninik Mudjihartini; Mohamad Sadikin; Sri Widia A Jusman
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.55

Abstract

Background: The kidneys in physiological conditions are always in a state of relative hypoxia. Cytoglobin (Cygb) is the newest globin protein found of the globin family. One of the functions of Cygb is in oxygen supply. Cygb expression is found to increase in hypoxic conditions, which are thought to be an adaptation response to hypoxia. Objective: This study aimed to analyze the expression of Cygb in rat kidneys which were exposed to chronic systemic hypoxia. Methods: Twenty five male Sprague-Dawley rats, weighing 150-200 g were used in this experiment. Rats were divided into 5 groups: The control group was exposed to normoxia; the hypoxia groups (10% oxygen / 90% nitrogen) for 1 day; 3 days; 7 days and 14 days. After treatment, rats were sacrificed and their kidneys were taken. Cygb mRNA expression was measured by qRT-PCR, while Cygb protein expression was measured by the ELISA method. Results: The expressions of Cygb mRNA and protein were found to be highest on day 3 of hypoxia and was correlated very strongly and significantly (r2 = 0.96; p <0.05). Conclusion: The highest expression of Cygb on day 3 of chronic systemic hypoxia exposure is suggested as an attempt to restore oxygen supply to the kidneys.
How to overcome the fear of coronavirus? Kholis Abdurachim Audah
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.61

Abstract

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