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INDONESIA
Indonesian Journal of Natural Pigments
Published by Ma Chung Research Center for Photosynthetic Pigments Publishing
ISSN : 23389281     EISSN : 26214245     DOI : -
Core Subject : Science, Education,
Chemistry Other
Indonesian Journal of Natural Pigments (Indones. J. Nat. Pigm.) is a double-blind international peer-review and open access journal (p-ISSN: 2338-9281, e-ISSN: 2621-4245) covering all aspects of natural pigments including biodiversity of natural pigment resources (isolation and analysis of photosynthetic pigments), pigments for food and healthcare, pigments for energy and pigments for sensors.
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 2 No 1 (2020): February 2020" : 5 Documents clear
OPTIMIZATION OF TOTAL CAROTENOIDS, PHENOLIC CONTENT, AND SENSORY ACCEPTABILITY OF JAVA TEA-BASED FUNCTIONAL DRINK ENRICHED WITH RED FRUITS’ OIL EMULSION Wijaya, Hanny; Natania, Natania; Sonatha, Mei Diana; A'yuni, Qurrata; Caroline, Cindy
Indonesian Journal of Natural Pigments Vol 2 No 1 (2020): February 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.1.1

Abstract

Red fruits’ oil contains 12,000 ppm carotenoid compounds and among them β-karoten was found as the major carotenoid compound. However, the application of red fruits’ oil as the food ingredient is rarely investigated due to its low solubility in aqua-phase, phenolic aroma and bitter aftertaste. Therefore , emulsification of red fruits’ oil in water (oil in water, o/w emulsion) using CMC (carboxymethyl cellulose) as the emulsifier and stabilizer agent considered as alternative to overcome those limitations. In the present work, the optimum concentration of red fruits’ oil emulsion added into Java tea-based functional drink increased the total carotenoid content, gave red color and might enhanced its antioxidant activity. The optimum formula was performed by Response Surface Methodology approach utilizing Design Expert 10.0.7® (DX10) Trial software whereas the emulsion concentration of red fruits’ oil as the variable factor. Red fruits’ oil emulsion which has been prepared from 20% red fruits’ oil and 1.50% CMC as emulsifier and stabilizer was having total carotenoid content of 72.93±1.33 ppm. The optimum concentration of red fruits’ oil emulsion added to Java-tea based functional drink was 5.86%. The addition of red fruits’ oil emulsion increased the total carotenoid content from 0.37±0.13 ppm to5.28±0.05 ppm. The results showed that red fruits’ oil emulsion could be used as red colorant agent in the functional drink while increasing the total carotenoid content, however, reduced the flavor sensory acceptance in high amount
Simple Photometer Development For Educational Purposes in Natural Pigment Analysis Kurniawan, Jovine M; Adhiwibawa, Marcelinus AS
Indonesian Journal of Natural Pigments Vol 2 No 1 (2020): February 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.1.17

Abstract

In this paper we will discuss the making of the simple photometer instrument to measure the absorbance of natural pigment crude extract. To make simple photometer, cuvette holder was printed using 3D Printing Machine, the development of simple photometer instrument also uses some components such as LED light (633 nm) as the light source, Photodiode-TSL250 as a detector and a variable resistor to set the initial intensity of light source. In this development, Arduino Uno was also used as USB data acquisition device to capture the signal from the instrument. The results of sample measurements between simple photometer instrument and UV-Vis 1800 spectrophotometer showed 98% regression coefficient of determination and simple photometer has a LOD and LOQ absorbance at 0.0267 and 0.0344. Simple photometer instrument was able to show similar response as commercial instrument. Through the development of simple photometer, student can easily understand the working principles of a spectrophotometer in natural pigment analysis that difficult to studied in detailed before.
Selection of Maceration Solvent for Natural Pigment Extraction from Red Fruit (Pandanus conoideus Lam) Purnomo, Tantyo Ardy Bintoro; Kurniawan, Yehezkiel Steven; Kesuma, Ruth Febriana; Yuliati, Leny
Indonesian Journal of Natural Pigments Vol 2 No 1 (2020): February 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.1.8

Abstract

Red fruit (Pandanus conoideus Lam) is rich with red-orange natural pigments, such as β-carotene. In this work, the solvent selection was investigated to extract the β-carotene from the red fruit via a simple maceration technique. Three types of solvents were used in the maceration, which were distilled water, ethanol, and acetone. The obtained extracts were characterized using spectrophotometer ultraviolet-visible (UV-visible), Fourier transform infrared (FTIR) and spectrofluorometer. The different solvents gave different spectroscopic information, suggesting that the solvent selection influenced the type of the extracted compounds. Among the examined solvents, acetone was found to be the most effective one to extract the β-carotene. The presence of the β-carotene pigment in acetone extract was confirmed by the appearance of the absorption peak at 476 nm on its UV-visible spectrum, while from its FTIR spectrum, the C-H sp3 functional group of β-carotene was found at 2924 and 2854 cm-1. In addition, the emission peak of β-carotene was found at 394 and 561 nm. This study confirmed that acetone performed as a better maceration solvent for β-carotene as compared to the distilled water and ethanol, which would be strongly related to the non-polar property of the acetone.
Intensity Ratio of LH2 Complexes from Rhodopseudomonas palustris and Rhodobacter sphaeroides for Purity Determination Prihastyanti, Monika NU; Kurniawan, Jovine M; Yusuf, Melisa M; Azmi, Sherly S; Ilmi, Mustafavi C; Indarto, Michelle L; Hoetomo, Stefan C; Wijaya, Melina E; Jatiyati, Greta; Brotosudarmo, Tatas H.P.; Heriyanto, Heriyanto
Indonesian Journal of Natural Pigments Vol 2 No 1 (2020): February 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.1.13

Abstract

Purification procedures to obtain light-harvesting complexes were performed on two photosynthetic bacteria, i.e. Rhodospseudomonas (Rps.) palustris and Rhodobacter (Rb.) sphaeroides. In this study, purification was initially carried out using series of centrifugation towards the bacteria cell to acquire chromatophore of the bacteria. Application of detergent (lauryldimethylamine oxide, or LDAO) to the chromatophore enabled the extraction of membrane containing photosynthetic apparatus. Further purification involved the application of sucrose density gradient centrifugation and ion exchange chromatography. The purity of collected LH2 complexes can be determined by calculating the ratio of AB850 : Aprotein. The resulting LH2 complexes from both bacteria showed relatively high ratio suggesting the purity of the complexes.
Thermal Degradation Kinetics of Encapsulated Palm Carotenes Using Different Combination of Wall Materials Hong, Khoo Mun; Indrawati, Renny; Brotosudarmo, Tatas HP; Limantara, Leenawaty
Indonesian Journal of Natural Pigments Vol 2 No 1 (2020): February 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.1.21

Abstract

Palm oil is known as one of the richest sources of carotenes. Carotenes concentrate could be prepared from crude palm oil and possessing potency to be used as nutraceutical materials. Due to the susceptibility of carotenes against heat, the encapsulation procedure might be applied to provide protections. The present study was aimed to learn the influence of using different combination of wall materials toward the thermal degradation kinetics of palm carotenes. Maltodextrin was used as primary wall material, being combined with various surface-active agents, i.e. Tween-80 (A), Tween-20 (B), and Arabic gum (C). The carotenes concentrate was encapsulated through emulsification steps, and the vivid yellow powder was obtained after freeze drying process. Then, encapsulated palm carotenes were stored in 200C, 350C, and 500C for several days, and the color, carotenes spectrum, as well as water content were monitored periodically. The kinetics of thermal degradation of carotenes were estimated using Arrhenius equation. The results showed that the use of different combination of wall materials exhibited distinct degradation rate. The combination of maltodextrin and Tween-80 gave preferable protection compared to the others, being half-degraded after 51 days in 200C.

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