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Dwi Anggorowati Rahayu
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Jurnal Riset Biologi dan Aplikasinya
Published by Universitas Negeri Surabaya
ISSN : -     EISSN : 26559927     DOI : 10.26740/jrba
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology
Jurnal Riset Biologi dan Aplikasinya is a peer-reviewed journal that strives to provide scientific information on the research results which focused on biological science (Ecology, Animal and Botanical Systematics, Microbiology, Zoology, Botany, Biotechnology, and Genetics and Evolution) and biological education (Innovation of Biology Learning, Assessment and Evaluation in Biology, and Media of Biology)
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 6 Documents
 1 
Search results for , issue "Vol. 5 No. 1 (2023)" : 6 Documents clear
Effects of Zinc Accumulation on Earthworm Aporrectodea caliginosa (Haplotaxida: Lumbricidae) Safaa Mohammed Mahmood; Adnan Musa Mohammed
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 1 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n1.p8-15

Abstract

Earthworms are key to the earth's ecosystem, which helps the soil increase its fertility and repair its existing elements, as well as remove contaminants. This study investigated the accumulation of Zn in the earthworm Aporrectodea caliginosa after 15 days of exposure. The worms were grown in media with different concentrations of Zn between 750 and 1500 ppm. Each treatment consisted of three replicates, containing 30 worms. A control group without Zn was also used. Data were analyzed by using Duncan Multi–Range. The results revealed that A. caliginosa had a strong ability to accumulate Zn in its tissue compared to the control group. It was noticed that the increase of the heavy metal in the worm´s tissue is associated with the elevation of the metal in its media. The results show a significant loss of weight in the worm´s body and loss in the growth rate; they also shown a decrease in specific growth rate. Moreover, there was a significant decrease in the worm length, showing a high effect, especially after 15 days of breeding with all concentrations used in this study. The researchers recommend using earthworms to purify the soil from contaminants because earthworm has a great ability to get rid of all pollutants, whether metals or pesticides or parasites, especially in industrialized countries and agricultural lands.
A Genetic Identification of Superbugs from River Streams in State University of Malang Reeno Al Hikmatus Sholekah; Dwi Listyorini
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 1 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n1.p27-36

Abstract

Superbugs revert to bacterial strains, which exhibit resistance to antibiotics. These bacteria could cause some problems in disease treatment and the environment. This research aimed of this study was to identify the presence of superbugs in the river stream that flows through Malang State University campus area. Amoxicillin, tetracycline, and chloramphenicol were selected to test the possible bacterial resistance. Water samples taken from a decided spot were spread over Luria Bertani’s agar on both antibiotic supplemented and antibiotics-free media with no delay. Molecular identification was carried out using 16S rRNA gene DNA barcoding approach, completed with morphological and Gram staining analyses. A total of 16 isolates of gram-negative colonies were found in the form of bacilli, diplobacilli, cocci, and diplococci. The genetic identification of eight resistant colonies led us to suggest that the isolates may belong to Aeromonas, Shigella, and Bacillus. Further studies are still required to get a clearer view of the correct taxonomical position of those resistant isolates.
Diversity of Moss Species (Bryophyta) In Senggani Ravine Tourism Area, Tulungagung Regency Desi Kartikasari; Gading Anon Widodo; Nur Habibah; Rakhmi Zahratul Asna
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 1 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n1.p43-51

Abstract

Moss plants (Bryophyta) are found in every habitat, and their presence in an ecosystem is controlled by environmental circumstances. The Senggani Ravine tourism area is a popular tourist attraction comprising a pine forest with extensive moss communities. This work aimed to assess the diversity of mosses (Bryophyta) in the Senggani Ravine tourism area for the first time. In June 2022, exploratory descriptive research of moss diversity was undertaken by a free walk around the Senggani Ravine tourism area from a predetermined position point (purposive sampling) using observation, documentation, literature study, and measurement of abiotic elements for data collection. Based on the results, twenty species of mosses were identified and can be divided into four classes, namely the Bryopsida, Polytrichopsida, Jungermanniopsida, and Marchantiopsida. The species identified were Barbulla indica, Fissidens purpusillus, Fissidens biformis, Fissidens biformis, Octoblepharum albidum, Rhizonium punctatum, Mnium hornum, Philonotis marchica, Fontinalis antipyretica, Hypnum cupressiform, Polytrichastrum formosum, Lejeunea flava, Lejeunea cavifolia, Bazzania prareupta, Bazzania vittata, Riccia junghuhniana, Marchantia emarginata, Marchantia polymorpha, Dumortiera hirsuta, and Lunularia cruciate. Abiotic factor measurements revealed that zone 3, which has a soil pH of 6, an air temperature of 24.1 C°, an 84% humidity level, and 200 Cd of light cm-1, is the most favorable area for moss growth. We can infer that the Senggani Ravine tourism area is still primarily undisturbed because the moss flora is still quite diverse and varied.
Leaf and Stomata Morphometrics of Gayam Inocarpus fagifer (Fabaceae) at Different Altitudes Alwi Smith; Kristin Sangur; Dessy Fitri Molle; Ludia Haurissa; Grisendy Maulany; Belsefren Renyaan
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 1 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n1.p16-26

Abstract

Gayam (Inocarpus fagifer) is one of the members of the angiosperm flora in Ambon City, Indonesia, that grows and develops at various altitudes. This research aimed to analyze the leaf and stomata morphometrics of these plants in the Aer Louw and Ema Village areas. Leaf samples were taken from the upper, middle, and lower strata and considered as replicates. The morphometric characteristics were measured manually using millimeter block paper and the formula for calculating leaf ratio. Furthermore, the stomata were stained using the direct incision method and safranin. The incision results were analyzed using an Olympus CX23 microscope at 400x magnification. The measurement and observation were analyzed descriptively and correlatively. The results showed that the average leaf width and length, also the midrib length were greater in Aer Louw Village than in Ema Village; while the leaf tip and stalk length were greater in Ema Village than in Aer Louw Village. The characteristics of stomata length and width in Ema Village were greater than in Aer Louw Village; otherwise, the number, index, and density of stomata in Aer Louw Village were greater than in Ema Village. Meanwhile, the correlational analysis showed that the environment influenced the variations of leaves and stomata. Therefore, the variations of leaves and stomata in the areas could predict plant adaptations to different environments.
Etlingera (Zingiberaceae) in Bogor Botanic Gardens: Potential Benefits and Its Conservation Status Yeyen Novitasari
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 1 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n1.p1-7

Abstract

Indonesia is one of mega biodiverse countries in the world, with a high global biodiversity index and many vascular plant species. However, not all plant species are known in terms of their uses, potential benefits, and conservation status. One of example is genus Etlingera, where further studies are required. Therefore, this study aimed to provide information on the potential benefits and uses of the genus Etlingera and to investigate its conservation status. The study was conducted using the method of literature study, an inventory of potential uses, and an inventory of the genus Etlingera living in Bogor Botanic Gardens through direct observation. Six species of the genus Etlingera were collected from the Bogor Botanic Gardens, namely Etlingera brevilabrum, E. elatior, E. hemisphaerica, E. loerzingii, E. megalocheilos, and E. walang, conservation status of three species (E. brevilabrum, E. hemisphaerica, and E. megalocheilos) is Least Concern (LC), two species (E. elatior and E. walang) are Data Deficient (DD), and one species (E. loerzingii) is Vulnerable (VU). All species are commonly used as spices, condiments, cosmetics, and traditional medicine to cure various diseases, possibly also as ornamental plants. The secondary metabolites present in some species, namely E. brevilabrum, E. elatior, and E. hemisphaerica can be used as antimicrobial, antifungal, and antioxidant agents.
Genetic Profile of Local Buffalo (Bubalus bubalis) Populations in Pacitan and Tuban, East Java, Indonesia Measured by the Molecular Marker of INRA032 Locus Laily Isnaini Rahmawati; Abdul Basith; Fitria Lestari
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 1 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n1.p37-42

Abstract

This study focused on the application of microsatellite markers at the INRA032 locus for genetic diversity assessment in buffalo (Bubalus bubalis) populations in Pacitan and Tuban Regencies, East Java, Indonesia. The total number of samples used was 16, each population represented by 8 samples. Genetic diversity assessment parameters include allele frequency, the Polymorphism Information Content (PIC) and heterozygosity. The results showed that based on the INRA032 locus, the Tuban buffalo population had a higher allele frequency range (0.08 to 0.33) than the Pacitan population (0.18 to 0.31). The average PIC value in both populations was 0.39, so it can be concluded that the INRA032 locus is informative enough to detect polymorphisms in both populations. The percentage heterozygosity of the Pacitan buffalo population is 88%, which is higher than the Tuban population at 50%, suggesting that the genetic diversity of the two populations is still quite high despite the decreasing trend in population numbers.

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