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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 20 Documents
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Search results for , issue "Vol. 30 No. 6 (2023): November 2023" : 20 Documents clear
α-Mangostin Effectively Inhibits Chikungunya Virus Replication in HepG2 Cells Moudy Soraya; Justus Sievers; Dionisius Denis; Anom Bowolaksono; R. Tedjo Sasmono
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1187-1193

Abstract

Chikungunya virus (CHIKV) is an arthropod-transmitted Alphavirus endemic to countries in Africa and Asia, including Indonesia, which causes debilitating arthralgia which can last several years. The rapid spread of CHIKV to new areas makes the discovery of antiviral agents a high priority. α-mangostin is a xanthone from mangosteen (Garcinia mangostana) pericarp and has antiviral activity against Hepatitis C and Dengue viruses. We investigated the antiviral activity of α-mangostin against CHIKV in HepG2 cells in pre-, post- and combination treatments compared to the common antiviral medicine ribavirin, as well their cytotoxicity. Our results show dose-responsive reductions in viral titer in all treatment regimes, with post- and combination treatments being more effective than pre-treatment only (IC50 = 7.79, 5.99 and 6.39 µM, respectively), but with poor specificity (SI = 1.39, 1.81 and 1.70, respectively) compared to ribavirin. Neither compound showed a direct virucidal effect. These results suggest α-mangostin effectively inhibits CHIKV replication in this cell line.
Diversity of Fungal Colonization in Respiratory Tract of Naïve Lung Cancer and The Emergence of Voriconazole Resistant Aspergillus Jamal Zaini; Abul A'la Al Maududi; Zahrah Annisa; Denny Grecius Siregar; Findra Setianingrum; Mulyati Tugiran; Ridhawati Sjam; Robiatul Adawiyah; Anna Rozaliyani; Sita Andarini; Elisna Syahruddin
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1139-1148

Abstract

Fungal spores in the air can be inhaled and enter the human respiratory tract. The entry of fungi into the respiratory tract can cause colonization or infection depending on the host immune response. Fungal colonization is the first step into debilitating fungal disease in humans, especially in immunocompromised groups. The increased rate of drug-resistant fungi has been reported in human disease and the environment. This study aims to examine the diversity of fungal colonization in humans and the rate of fungal resistance to voriconazole. This cross-sectional study was done in patients with naïve lung cancer who had not been previously treated with any cancer therapy nor given antifungal agent. Induced sputum from 70 subjects was collected and inoculated in the Sabouraud Dextrose Agar medium. Macroscopic and microscopic examinations were performed to identify fungal species. Voriconazole susceptibility tests were done using the disc diffusion method. This study found Candida albicans, Aspergillus niger, Aspergillus fumigatus, and Penicillium sp. among the most common lower respiratory tract colonies. This study also found the colonization of up to 5 species in a single subject. A high rate of voriconazole-resistant Aspergillus sp. was found (42.4%) among 59 isolates tested. Given that these subjects had never taken antifungal agents previously, the high rate of voriconazole resistance might be attributed to the environment, such as community and agriculture. Mitigation of antifungal use in the agricultural sector, fungal diversity in the environment, and clinical study of fungal colonization/ infection in other high-risk groups are needed.
Assessment of Teratogenic Effects of Sappan Wood (Caesalpinia Sappan L.) Extract In Rats (Rattus novergicus) Jeri Nobia Purnama; Erick Khristian; Mas Rizky A.A Syamsunarno; Yusof Kamisah; Ratu Safitri
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1036-1042

Abstract

Treatment using plant materials has been widely researched and observed to assist in curing a disease. Medicinal products derived from herbal plants are proven useful as drugs must follow guidelines not to cause acute or chronic toxicity. The purpose of this study was to investigate the teratogenic potential of an ethanolic sappan wood extract on the growth of unborn rats. Six groups of pregnant rats are created: Aquadest was administered to the Control group, whereas the Treatment groups received 100, 200, 300, 400, or 500 mg.kg-1 BW of an ethanolic extract of Sappan wood. On the twenty-first day of gestation, pregnant rats were CO2-euthanized and delivered vaginally. Rats' body weight during pregnancy was observed, along with fetal growth measurements, viscoelastic examination, and skeletal development. Wistar rats' reproductive systems, fetal body weight, body length, and tail length were all unaffected by ethanol extract of Sappan wood at doses from 100 mg/kg to 500 mg/kg. In fetuse rat from the control group and the five dosage groups, no skeletal development had taken place and there were no obvious external abnormalities. Even at the maximum dose of 500 mg, sappan wood extract demonstrated no teratogenic effects on the development of fetal rats.kg-1 BW.
Melissopalynology and Vegetation Analysis Surrounding Sunggau of Giant Honey Bee Apis dorsata in Belitung Regency Dwika Bramasta; Ibnul Qayim; Nina Ratna Djuita; Rika Raffiudin; Ramadhani Eka Putra; RC Hidayat Soesilohadi; Hery Purnobasuki
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1167-1174

Abstract

The forest conversion into oil palm plantations in Belitung impacts the plant source for pollen and nectar to support the honey bee Apis dorsata. This study aimed to identify the plants used by A. dorsata as pollen sources in honey and bee bread in honey bee nests and to analyze the vegetation composition and structure surrounding the sunggau (artificial nesting site) in Belitung Regency. Honey from A. dorsata was collected from bee nests in sunggau on Kampak Island and Tanjung Rusa. The pollen grains from 20 ml honey were acetolysed and counted until 1,200 grains for each honey sample. In Kampak Island, we found eight pollen types in honey dominated by Rhizophora mucronate mangrove pollen and eleven pollen types in bee bread dominated by Melaleuca cajuputi. The pollen type in honey in Tanjung Rusa was similar to those in Kampak Island, and nine pollen types were found in the bee bread dominated by Elaeis guineensis. The vegetation analysis revealed that mangrove and heath forests in Kampak Island were dominated by Lumnitzera littorea and Melaleuca cajuputi, respectively. The results of this study confirm the bees' notable use of the mangrove ecosystem, which adds conservation value, especially in supporting bee management efforts in Belitung.
Spermicide of Papaya Seed Oil and Compounds I Made Sukadana; Sri Rahayu Santi; Ni Putu Lisna Oktaviani
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1111-1120

Abstract

This study aimed to determine the spermicidal activity of papaya seed oil (Carica papaya L) from local Bali varieties against Wistar rat spermatozoa by evaluating sperm quality and the correlation between the percentage of motility and viability spermatozoa to identify its active compounds. Papaya seed oil was obtained by extracting papaya seeds using n-hexane solvent. The study was designed as follows; group of spermatozoa Wistar rat were treated with NaCl 0.9% (control, P0), with Sodium carboxy methyl cellulose (NaCMC) 0.5% (P1), with n-hexane extract 0.1%(b/v) (P2), with n-hexane extract 0.3%(b/v) (P3), and with n-hexane extract 0.5%(b/v) (P4). Column chromatography was used to separate the n-hexane extract into two fractions, A and B. The spermicide test for fractions A and B followed the same design as the test for n-hexane extract but until the P7 group. The result showed that fraction B exhibited higher spermicidal activity, with 23.3% motility and 33.8% of viability sperm at 100 ppm. The identification of fraction B by LCMS showed at least 12 mixture compounds were venoterpine, vitamin K5, 5-phenyl-2-pyridinamine, harmalan, styrylquinoline, 1,2,3,4-tetrahydro-1-benzoyl-2-methyl-4-phenylamino-quinoline, calcitroic acid, siderol, N-benzyl-linoleamide, (3β)-3-(diisopropylamino)androst-5-en-17-one, pipereicosalidine, and 25-azacholesterol. Spermicidal activity may be a synergistic effect of several compounds that have a cytotoxic activity such as styrylquinoline, 1,2,3,4-tetrahydro-1-benzoyl-2-methyl-4-phenylamino-quinoline, siderol, and pipereicosalidine.
Population Growth Model and Mortality of Pakistan Lobster (Panulirus polyphagus) in Estuary Waters of Tarakan City Agus Indarjo; Gazali Salim; Lukman Yudho Prakoso; Budi Pramono; Meiryani; Thonas Indra Maryanto; Suriyanti; Muhammad Firdaus; Rozi
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1043-1053

Abstract

Lobster Panulirus polyphagus has a fairly high economic value and is found in the estuary waters of Tarakan City. This research aims to study the growth and mortality model of Lobster P. polyphagus originating in the estuary waters of Tarakan City. The research method was carried out using a quantitative descriptive method. Sampling was conducted 14 times from December 2021-May 2022 using gill nets. The results showed that the male sex ratio was more than the female. Allometric growth of males and females is negative allometric with a thin body shape. The structure of the size obtained was mostly in males ranging from 18.3-20.6 cm, and females around 20.5-22.2 cm. The maximum length growth of P. polyphagus based on von Bertalanffy's growth model was 31.519 cm in males and 31.374 cm in females. The total mortality (Z) of P. polyphagus for males and females was 1.104 and 1.119; catch mortality (F) of 0.106 and 0.253; natural mortality (M) of 0.998 and 0.866; exploitation rate (E) of 0.096 and 0.226, respectively. The high natural mortality causes the extinction of the Lobster species, so good management is needed so that it is sustainable.
Cardioprotective Activity of Costus Root Ethanol Extract in Experimentally-Induced Hypothyroidism in Female Albino Rats Mohammed Rashed Abdul; Saleh Mohammed Rahim; Ahmad Hamad Saleh
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1054-1060

Abstract

Hypothyroidism is a popular endocrine disorder caused by a thyroid hormone deficit. Changes in the state of the thyroid gland significantly affect many organs, including the heart. This study examined the cardioprotective effects of an artificially induced hypothyroidism in female rats using an ethanol extract from the root plant Saussurea lappa (Costus). For the current study, 25 adult female albino rats were employed. They were split into five equal groups, including control, hypothyroid group, post-treatment group with costus extract, co-administered hypothyroid group with costus extract, and post-treatment group with levothyroxine. Thyroid hormones (T3, T4, and TSH), lipid profile, and oxidative stress indicators (catalase, superoxide dismutase, reduced glutathione, and lipid peroxidation levels) were also measured in the serum. The heart muscle was the subject of a histological research. The findings suggested that Costus root ethanol extract improved hypothyroidism in female rats, as proven by the reversal of many biochemical abnormalities and improvements in the heart's histology. Our study indicates that the root of Costus exhibited cardioprotection efficiency against hypothyroidism-induced serious effects on the heart, The antioxidant and radical-scavenging properties of its components may be responsible for this promising effect.
Genetic Diversity and Population Structure Analysis of Potato Somaclones Nina Agusti Widaningsih; Ika Roostika; Dwinita Wikan Utami; Awang Maharijaya; Kusmana; Usep Jenudin; Sobir
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1008-1016

Abstract

Cultivated potato varieties are tetraploid and vegetatively propagated. Therefore genetic improvement for new traits is less effective through hybridization. The mutation is considered an alternative method for crop improvement of those varieties. This study aimed to evaluate the effects of gamma-ray irradiation on increasing genetic diversity among the somaclones and the changes in population structure. Forty-four somaclones were used as observed materials. The population was grown in a screen house using a completely randomized design with genotype (somaclone) as a single factor. Thirteen qualitatively and quantitatively morphological characters were observed. Six SSR markers were used for analyzing the population structure. The selection process was based on a weighting method divided into quartiles. Selected numbers were taken from quartile 3. Morphological analysis of stems and leaves resulted in five different somaclones, with significant differences in anthocyanin appearance and intensity of the green color of the leaflets. Diversity criteria based on quantitative characteristics showed a high level of diversity with a high heritability for tuber weight, length, and diameter and a moderate heritability for tuber number. Analyzing the population structure has offered insight into how gamma irradiation affected the somaclones. As a result of the selection, 12 somaclones met the requirements to serve as seed sources for field testing. It is expected that the results of this study will provide information about the diversity caused by gamma-ray irradiation treatment on potato somaclones and a method for improving the efficiency of the initial selection of potato populations.
Molecular Docking and Dynamics of SARS-CoV-2 Programmed Ribosomal Frameshifting RNA and Ligands for RNA-Targeting Alkaloids Prospecting Adhityo Wicaksono; Arli Aditya Parikesit
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1025-1035

Abstract

RNA-ligand docking is a part of computational biology, which is currently lowly recognized compared to the protein-ligand docking procedure commonly applied for drug discovery. This in silico study aims to create a simplified protocol for RNA-ligand docking, which is applicable to RNA-targeting small molecular drug screening. Four alkaloids (berberine, colchicine, nicotine, and tomatine) were subjected to this study and contended against the SARS-CoV-2 genomic RNA -1 PRF component targeting control drug, merafloxacin, including two known intercalator berberine and colchicine, a small alkaloid nicotine and a large alkaloid tomatine. The alkaloids were screened for drug-likeness properties (Lipinski’s Rules of 5 or LRo5), bioavailability indexes, and synthetic accessibility values using SwissADME before docking. The docking used PyRx – Autodock Vina and re-scored for RNA-ligand scoring using AnnapuRNA. The docking results have the interactions mapped using fingeRNAt and visualized using Discovery Studio. Molecular dynamics using CHARMM36 and AMBER forcefields were simulated in NAMD. The molecular dynamics 1 ns simulation results showed that the ligand interaction over time did not cause much interference with the RNA, indicated by the low number of RMSD changes between RNA itself and the RNA-ligand complex. Additionally, CHARMM36 forcefield provided more stable fluctuation compared to AMBER. The results indicated that tomatine disobeyed LRo5 and had a low bioavailability index and bad synthetic accessibility value, while the rest alkaloids passed. In the end, berberine has an even higher docking score than the control drug. The study also shows that this protocol can be useful for future RNA-ligand computational studies.
The Properties of Exosomes Derived from Mesenchymal Stem Cells Preconditioned with L-Ascorbic Acid and Cobalt (II) Chloride Anggraini Barlian; Rizka Musdalifah Amsar; Salindri Prawitasari; Christofora Hanny Wijaya; Ika Dewi Ana; Triati Dewi Kencana Wungu
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1100-1110

Abstract

Extracellular vesicles including exosomes, are produced by cells for intracellular communication. Preconditioning of parental cells influences exosome properties. The purpose of this study was to examine the effects of L-ascorbic acid (LAA) and cobalt (II) chloride (CoCl2) on human Wharton’s jelly mesenchymal stem cell (hWJ-MSC)-derived exosomes and their ability to promote stem cell differentiation into chondrocytes. The cells were isolated from the umbilical cord and characterized according to the criteria for mesenchymal stem cell. The cells were cultured in a serum-free medium containing LAA and CoCl2. Cell-produced exosomes were isolated and characterized. hWJ-MSCs can grow in serum-free medium containing LAA and CoCl2. Exosomes derived from hWJ-MSCs had a round morphology, particle size within the exosome range, CD 63 expression, and the capacity to be internalized by cells. The production of exosomes by hWJ-MSCs was enhanced by LAA treatment. LAA and CoCl2 promoted stem cell differentiation into chondrocytes, as indicated by the production of collagen type II and glycosaminoglycans. LAA and CoCl2 affect the properties of MSC-derived exosomes. LAA induces cells to produce exosomes in greater quantities, which have the potential to promote chondrogenic differentiation of stem cells.

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