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Current Research on Biosciences and Biotechnology
Published by Institut Teknologi Bandung
ISSN : -     EISSN : 26861623     DOI : https://doi.org/10.5614/crbb
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology
The Current Research on Biosciences and Biotechnology (CRBB) encourages researchers to publish works related to the use of the biosciences and biotechnology in understanding the world around us. From Health and Medicine to Advanced Materials, these state-of-the-art research offer novel insights in a multidisciplinary environment. We encourage scientists to submit papers focusing on the following broad topics: Health and Medicine Pharmaceutical Sciences Pharmacology and Toxicology Food Microbiology and Biotechnology Biotechnology Agriculture Bio-Energy Marine Sciences Advanced Materials Environmental Sciences
Arjuna Subject : Umum - Umum
Articles 55 Documents
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Mitragynine: a review of its extraction, identification, and purification methods Amrianto; Sumail Sidik Ode Ishak; Nanda Putra; Syefira Salsabila; Laode Muhammad Ramadhan Al Muqarrabun
Current Research on Bioscences and Biotechnology Vol. 3 No. 1 (2021)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2021.3.1/TMPNSA4H

Abstract

Mitragynine is one of the dominant alkaloids present in Mitragyna speciosa. The compound possesses several pharmacological properties such as antinociceptive, anti-inflammatory, and anti-cancer. Studies have reported various methods in extracting mitragynine, both conventional and renewable technology combined with acid-base techniques for the enrichment and purification of mitragynine from M. speciosa. Several chromatography and spectroscopy instruments such as HPLC, LC-MS, GC-MS, and NMR have been used to identify mitragynine and its content in both the extract and fraction mixtures. In this review, we aim to provide insight on how the methods of extraction, purification, and identification of mitragynine have been developed over the last few decades. This report shows comparison among the various approaches in extracting mitragynine and points out the facts that different methods gave different yields of the compound.
Proximate and biological activity studies of Syzygium samarangense: Study of different extractives of Syzygium samarangense Tirthankar Biswas; Md. Al-Amin; Mohammad Shoeb; Md. Kamrul Hasan; Md. Nazrul Islam; Md. Mizanur Rhaman
Current Research on Bioscences and Biotechnology Vol. 3 No. 1 (2021)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2021.3.1/NKTEH76W

Abstract

Red and green varieties of Syzygium samarangense, commonly known as Jamrul, are very popular as seasonal fruits to all ages of people in Bangladesh. Objective of the work was chemical and biological activity studies of S. samarangense red and green varieties grown in Bangladesh. Air-dried fruits of S. samarangense were successively extracted with n-hexane, dichloromethane (DCM) and methanol. The moisture and ash content of the raw fruits were determined by thermal heating at 105 and 700℃, respectively. Different biologically important functional groups were identified by FT-IR spectroscopy. The n-hexane, DCM and methanolic extracts were tested for cytotoxicity against HeLa and Vero cell lines. Total phenolic content, total flavonoid content and total antioxidant capacity in the three extracts of both varieties were determined. The moisture content and ash content were 92% and 90%, and 0.12% and 0.16% in red variety and green variety, respectively. UV-Vis spectrum revealed the presence of long chain conjugation or polycyclic aromatic chromophores in n-hexane, DCM and methanolic extracts of S. samarangense. The DCM extract of both varieties had highest total phenolic content, flavonoid content and antioxidant capacity compared to n-hexane and methanol extracts. The study concludes that the fruit of S. samarangense (red and green variety) grown in Bangladesh are rich source of phytochemicals which possess antioxidant activity and safe to health for their non-cytotoxic properties. The green variety contains more phenolic content and antioxidant capacity than red variety.
Ursodeoxycholic acid: a systematic review on the chemical and biochemical properties, biosynthesis, sources and pharmacological activities Marlin Megalestin Raunsai; Elfahmi; Agus Chahyadi; Sony Suhandono; Tati Kristianti; Laode M.R. Al Muqarrabun
Current Research on Bioscences and Biotechnology Vol. 3 No. 1 (2021)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2021.3.1/UJZVBNA0

Abstract

Ursodeoxycholic acid (UDCA), a secondary bile acid (BA), is an acidic steroid synthesized from cholesterol in hepatocytes. UDCA is widely used for the treatment of various diseases related to liver injury. The use of UDCA to treat non-liver diseases has also been developed recently, such as neurodegenerative diseases, cancer, and obesity. Due to the important role of UDCA on human health, numerous studies in understanding its chemical and pharmacological properties have been published. Literature sources were obtained from online databases such as Science Direct, Google Scholar, Scopus and PubMed using keywords relating to the purpose of study. Critical analysis and review were performed for all literature. UDCA is a steroid compound with pharmacological properties. Seventeen enzymes are involved in its biosynthesis, which has been proposed in four pathways: classic, alternative, the Yamazaki, and 25-hydroxylation pathways. UDCA can be isolated from bovine bile, bear bile or all Ursidae, human, rabbit, cow, rat, hamster, sheep, pig, and plant. UDCA has been used in the treatment of several diseases such as primary biliary cirrhosis, intrahepatic cholestasis of pregnancy, hepatolithiasis associated with Caroli syndrome gallstones, cystic fibrosis, hepatitis C virus, chronic heart failure, neurodegenerative diseases, and obesity, as well as in the prevention of cancer. UDCA has a wide range of pharmacological properties. Further investigations on its efficacy and safety on humans are required before it could be used for several indications. All genes which are responsible in UDCA biosynthesis have been elucidated. That said, further genetic engineering studies in order to find other prospective sources of UDCA could be a challenge for the future research.
Molecular docking-based virtual screening of antidiabetic agents from Songga (Strychnos lucida R.Br.): an Indonesian native plant Arif Setiawansyah; Muhammad Andre Reynaldi; Daryono Hadi Tjahjono; Sukrasno
Current Research on Bioscences and Biotechnology Vol. 3 No. 2 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.3.2/82KYTCPW

Abstract

This study was carried out to predict the compounds derived from Songga that have potential as antidiabetic and predict their mechanism of action on various pathways of glucose regulation in diabetes mellitus by molecular docking. Molecular docking-based virtual screening was done by using AutoDock Vina software assisted by AutoDockTools. The test compounds used for virtual screening were obtained from literature studies and were combined with Lipinski’s rule to select the compounds for the prediction of lead candidates that can be used in oral administration. The receptors used in this study were human aldose reductase, human maltase-glucoamylase, PPAR-gamma, pancreatic beta-cell SUR1, and human DPP-IV. The validation of the molecular docking method of five target receptors showed that RMSD values of human aldose reductase, human maltase-glucoamylase, PPAR-gamma, pancreatic beta-cell SUR1, and human DPP-IV were 0.6446 Å, 1.8668 Å, 0.2527 Å, 1.7452 Å, and 1.7439 Å, respectively. From the molecular docking-based virtual screening, we discovered that for each target protein, there were one to three optimal compounds that have the best interaction in our investigation. Those compounds were chlorogenic acid on human aldose reductase, phyllamycin A, chlorogenic acid, and brucine N-oxide on human maltase-glucoamylase, phyllamycin A on PPAR-gamma, strychnine N-oxide on pancreatic beta-cell SUR1 and strychnine on human DPP-IV with binding affinity value of -9.9 kcal/mol, -7.6 kcal/mol, -9.9 kcal/mol, -8.8 kcal/mol, and -6.2 kcal/mol, respectively. Several compounds are predicted to have potential to be developed as antidiabetic agents. However, further laboratory investigations like in vitro and in vivo assays need to be conducted.
Ethnopharmacognosy study, antioxidant activity, and chemical content in chicken bile Slamet Tuty; Irda Fidrianny; Sukrasno
Current Research on Bioscences and Biotechnology Vol. 3 No. 2 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.3.2/UFG27H3H

Abstract

The aim of this research was to study the ethnopharmacognosy, antioxidant activity, and chemical content of chicken bile. In addition, a bioactivity-guided isolation was also performed to identify compounds in chicken bile having antioxidant activity. Extraction was carried out by reflux method. Each extract was tested for its antioxidant activity using DPPH and CUPRAC methods. Fractionation was performed on selected extract, namely ethyl acetate extract, using vacuum liquid chromatography and subfraction using preparative TLC method and classical column chromatography (CCC). Compound 1 was characterized and identified by NMR, compound 2 was characterized and identified by LC-MS/MS and NMR, subfraction 7 was characterized by GC-MS. The result of the antioxidant activity of ethanol extract of chicken bile using the DPPH method (IC 50: 46.64 g/mL), ethyl acetate extract (IC 50: 69.99 g/mL), n-hexane extract (IC 50: 71.65 g/mL, fraction 15-17 (IC 50: 88.77 g/mL) showed strong antioxidant, whereas by CUPRAC method all extracts (EC 50: 69.90, EC 50: 80.28, EC 50: 70.40) and fraction (EC 50: 64.53) are strong antioxidants. The result of identification of compound 1 by NMR was identified as cholesterol, identification of compound 2 by LC-MS/MS was (E)-hexadecyl-ferulate and subfraction 7 which was identified by GC-MS produced dominant compounds, namely oleic acid, n-hexadecanoic acid, octadecanoic acid and palmitoleic acid. In conclusion, extract, fraction 15-17 and subfraction of chicken bile have antioxidant activity where the active antioxidant compound is a phenol group compound, namely (E)-hexadecyl-ferulate. The other chemical constituents found in chicken bile are, oleic acid, n-hexadecanoic acid, octadecanoic acid, and palmitoleic acid.
Investigation of tetracycline residues in poultry meat samples from Dhaka city by high-performance liquid chromatography Nargis Parvin; Mohammad Shoeb; Nilufar Nahar; Rafiza Islam
Current Research on Bioscences and Biotechnology Vol. 3 No. 2 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.3.2/P89JJ95N

Abstract

The objective of the study was to quantify tetracycline (TCs) i.e., oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) residues in thirty poultry meat samples (n = 30) collected from the local market and super shop around the Dhaka University campus during May 2019 to January 2020. Three samples were collected from each of ten locations. All samples were extracted by Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method. Samples were analyzed by reversed-phase High-Performance Liquid Chromatograph equipped with Photo Diode Array Detector (LC-PDA) and a reported method was validated with good linear correlation coefficients of standards and matrix-matched calibration curves with r2=1.00, 0.99, 0.99 and r2= 0.99, 0.99,0.99 in the linearity range of 0-10 µg/kg for OTC, TC and CTC, respectively. The limit of detection (LOD) and limit of quantification (LOQ) for OTC, TC, and CTC were 1.05, 1.17, and 1.09 μg/kg and 3.15, 3.51 and 3.27 μg/kg, respectively. Accuracy that is expressed by the recovery percentages were calculated at two different concentrations (2.5 and 5 μg/kg) were 91 and 100%, 102 and 100%, and 106 and 100% for OTC, TC, and CTC, respectively. Intra-day (n=3) and inter-day (n=9, 3 days) precision data were under 10% for all sample matrixes. Standard deviations were calculated ±0.06, ±0.11 and ±0.03 and precision (expressed by RSD%) were found 5.57, 9.14 and 2.35%, respectively for OTC, TC and CTC. The HPLC-PDA method is affordable for screening of large number meat samples for residual antibiotics in biological matrices by any laboratories. The method is also cheaper in comparison with LC-MSMS. Analysis of real 30 poultry meat samples showed that the tetracyclines residues were below the quantification limit in all samples.
Determination of age-dependent endogenous indole-3 acetic acid (IAA) level in different organs of tomato plants Tasnim Ahmed; Rafiza Islam; Nargis Parvin; Mohammad Shoeb
Current Research on Bioscences and Biotechnology Vol. 3 No. 2 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.3.2/WFP0HMM1

Abstract

Indole-3-acetic acid (IAA), a phytohormone, is a crucial modulator of plant growth and development. As increased levels of endogenous IAA is responsible for inhibitory effects on plants, knowing its concentration would be helpful for controlling exogenous IAA input. An optimum endogenous IAA level is required for proper growth and development of plant with good health status. Hence, in this study, we have detected and measured IAA present in tomato root, shoot, leaves and fruits at two different ages (25 days and 50 days) by Reversed-Phase High Performance Liquid Chromatography (RP-HPLC), a suitable chromatographic method for separating molecules based on hydrophobicity. We observed that the endogenous level of IAA was significantly higher in roots, shoots, leaves and fruits at 50-days old plant compared to 25-days old mature plant (p-value <0.01). These results suggested that the IAA level may increase with the increase of the age of tomato plants. The tomato plants showed good vigor with IAA range 0.3-3.0 µg/g.F.W (25 days) and 3.4-7.5 µg/g.F.W (50 days) of age, respectively, implying that IAA might be used as a possible plant health indicator. Further study is needed to manipulate the IAA concentration for adequate plant growth and development.
HPLC analysis of artificial preservatives, stimulants and sweeteners in carbonated beverages in Bangladesh Mohammad Shoeb; Md. Mafizul Islam; Md. Shahed Reza; Nilufar Nahar; Md. Mazharul Islam
Current Research on Bioscences and Biotechnology Vol. 3 No. 2 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.3.2/2V2BWDB5

Abstract

Different (n=21) commercial carbonated beverages, available in Bangladesh, were analysed by HPLC coupled with a photodiode array detector (UV-PDA). The main objective of this study was to quantify the amount of different artificial sweeteners, stimulant and preservatives and to validate the two methods used in this purpose. The two methods are simultaneous determination of sodium benzoate, caffeine, and saccharin using the sodium acetate buffer with pH 3.0 at 254 nm and simultaneous determination of artificial sweeteners acesulfame-K and aspartame using the potassium phosphate monobasic buffer. Correlation coefficient (r2) obtained were 0.9977, 0.9976, 0.9975, 0.9993 and 0.9956 in the range of 1-100 µ/L, recoveries were 95.88-97.10, 96.53-97.20, 94.53-96.05, 91.15-93.31 and 92.88-94.13%, LODs were 30.06, 15.46, 31.61, 0.33 and 0.83 mg/L, and LOQs were 100.18, 51.54,105.37, 1.11 and 2.77 mg/L for saccharin, caffeine, sodium benzoate, acesulfame-K and aspartame, respectively. The present analysis shows that amount of saccharin, caffeine, sodium benzoate, acesulfame-K and aspartame in the range of ND-377.60, ND-462.36, ND-217.60, ND-48.09 and ND- 295.20 mg/L, respectively. The total carbohydrate found as 102.81-147.16 g/L using the UV-visible spectrophotometric method which implies that sample containing more amount of carbohydrate has less artificial sweeteners value.
Isolation of bioactive compounds with tyrosinase inhibitory activity from the methanol extract of meniran herb (Phyllanthus niruri Linn.) Syefira Salsabila; Rika Hartati; Laode M.R. Al Muqarrabun; Nurinanda Prisky Qomaladewi; Ariranur Haniffadli; Andi Rifki Rosandy; Agus Chahyadi; Elfahmi
Current Research on Bioscences and Biotechnology Vol. 3 No. 2 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.3.2/GOO6ZULV

Abstract

Meniran (Phyllanthus niruri L.) is a wild plant in the Euphorbiaceae family which grows in tropical climates. The plant is known to possess antioxidant activity and is rich in phenolic compounds. Since plants with high phenolic content and high antioxidant activity are known to exhibit high tyrosinase inhibitory activity, the purpose of this study is to determine the inhibitory activity of the tyrosinase enzyme by the methanol extract of meniran (P. niruri L.) and to isolate secondary metabolites from the extract and fractions which exhibit inhibitory activity against tyrosinase. The in vitro tyrosinase inhibitory activity assay was conducted using the 96-well microplate method with kojic acid as the positive control, while the separation and purification of compounds were carried out using chromatography techniques. The methanol extract and n-hexane fraction showed tyrosinase inhibitory activity with IC50 of 11.05±0.16 and 2.57±0.08 mg/ml, respectively. Three bioactive compounds were succesfully isolated from the n-hexane fraction. Based on the UV and NMR spectra, compounds 1, 2, and 3 were identified as phyllanthin, phyltetralin, and hypophyllanthin, respectively. Furthermore, phyllanthin (1) was shown to demonstrate inhibitory activity against tyrosinase with IC50 of 0.6322 mM. In conclusion, both meniran extract and phyllanthin (1) were found to exhibit inhibitory acitivity against the tyrosinase enzyme. In the future they can be candidates for the treatment against hyperpigmentation and also as an active ingredient in skincare formulations.
Total phenolic, flavonoid, and antioxidant capacity of bajakah (Spatholobus littoralis Hassk) Muhammad Ikhlas Arsul; Nurfajri Syamsi; Nadila Putri; Nur Afiah Alfrianti Nur; Mukhriani; Nursalam Hamzah
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/VRJ3X4LF

Abstract

The bajakah (Spatholobus littoralis Hassk.) is one of the typical plants from Kalimantan Island, which has potential as a drug, one of which using as an antioxidant is due to the secondary metabolites contained therein. The aims of the research are to estimate the total phenolic content, total flavonoid content, and antioxidant capacity of the bajakah by different methods. Extraction was carried out by reflux method using a solvent gradient of n-hexane, ethyl acetate, and ethanol 96%. Phenols and flavonoid content were calculated using spectroscopy. Antioxidant capacity was measured against ascorbic acid and Trolox using the CUPRAC, DPPH, and FRAP methods and reported as mg Trolox equivalent (TEAC) and ascorbic acid equivalent (AEAC) per gram extract. The ethanol extract showed the highest antioxidant capacity for the CUPRAC and FRAP methods, the n-hexane extract for the DPPH method on both varieties of bajakah tampala. Each method provides significantly different antioxidant capacity values. The CUPRAC and FRAP methods found the highest antioxidant capacity in the white variety of bajakah ethanol extract. Meanwhile, in the DPPH method, saw the highest antioxidant capacity in the red variety of n-hexane bajakah extract. DPPH and CUPRAC give a positive correlation with TPC, where CUPRAC assay produced higher values than FRAP and DPPH assays.

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