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Jurnal Sains dan Teknologi Laboratorium Medik
Published by Akademi Kesehatan John Paul II Pekanbaru
ISSN : 25275267     EISSN : 26217708     DOI : https://doi.org/10.52071/jstlm
Core Subject : Health, Science, Education,
Education Health Professions Immunology & microbiology Public Health Other
Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM, e-ISSN: 2621-7708, p-ISSN: 2527-5267) adalah sebuah jurnal yang didedikasikan untuk plublikasi hasil penelitian yang berkualitas dalam bidang sains dan laboratoium medik. semua publikasi di jurnal Jstlm bersifat akses terbuka yang memungkinkan artikel tersedia secara bebas online tanpa berlangganan apapun. Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) dikelola secara profesional dalam hal membantu para akademisi, peneliti dan praktisi untuk menyebarkan hasil penelitiannya. focus dan scope Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) adalah sains, mikrobiologi, parasitologi, hematologi, kimia klinik, dan toksikologi.
Arjuna Subject : Umum - Umum
Articles 8 Documents
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Search results for , issue "Vol 9 No 1 (2023): April (2023)" : 8 Documents clear
ROSELLA (Hibiscus sabdariffa) SEBAGAI ALTERNATIF BAHAN PEWARNA HISTOLOGI Yeni Rahmawati; Saraswati Saraswati
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v8i2.113

Abstract

ABSTRACT Hematoxylin-Eosin (HE) is a synthetic dye that is commonly used in histological examination. HE exposure in the long term has a negative impact on health. The development of alternative dyes is important. Therefore this literature study aims to determine the use of rosella (Hibiscus sabdariffa) in histological staining. Literature review was conducted from five data sources, namely Google Scholar, Medline, Oxford Academic, Research Gate and Science Direct. Based on a literature search, 10 journals were obtained which showed that rosella (Hibiscus sabdariffa) can be used as an alternative dye for various types of human or animal histological tissues. Extraction can be done on fresh or dry leaves and flower petals. Solvents that can be used in the extraction process are polar solvents, namely distilled water or alcohol. Extraction methods of soxhletation, reflux, maceration or boiling can be used in the extraction of rosella (Hibiscus sabdariffa). Staining can be done using pure or modified extracts. Various variations of extraction methods, types of solvents, concentrations and staining times, as well as the degree of acidity performed can give staining results that are as good as HE. Rosella (Hibiscus sabdariffa) can be used as an alternative to HE dye. Keywords: Rosella, Hematoxylin Eosin, staining, histology
Uji Daya Hambat Sari Bawang Putih (Allium Sativum) Terhadap Pertumbuhan Jamur Malassezia furfur Menggunakan Metode Difusi Kertas Cakram chelyn mareli sarungu
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.120

Abstract

Malassezia furfur is a dimorphic fungus that is lipophilic and causes pityriasis versicolor. People who experience pityriasis versicolor usually treat with oral treatments such as ketocenazole, itraconazole, fluconazole and tropical treatments such as 2.5% sulfide shampoo. Treatment like this is not free from side effects if there is repeated infection and long-term use of drugs without a doctor's prescription so that it can cause resistance. Therefore, other alternative treatments use natural medicines. One of them is garlic (Allium Sativum). Garlic has the main active compound content is Allicin, then other active compounds are essential oils, alkaloids, tannins, saponins, and flavonoids. The purpose of the study was to determine the ability of garlic (Allium Sativum) at various concentrations of 70%, 75%, 80%, 85%, 90% against the growth of Malassezia furfur fungus. The research design is true experiment post test only with control. This study uses the agar diffusion method with paper discs. The results of this study are garlic juice (Allium Sativum) concentration of 80% has the ability to inhibit the growth of Malassezia furfur with an inhibition zone of 46.23 mm which has effectiveness with imidazole ketocenazole group drugs.
STUDI KASUS : GAMBARAN KARAKTERISTIK PENDERITA PENYAKIT INFEKSI MENULAR SEKSUAL AKIBAT BAKTERI NEISSERIA GONORRHOEAE DI PUSKESMAS MLATI 1 TAHUN 2022 Mezi Putri Arifani; Nadia Nanda Safitri; Nazula Rahma Shafriani; Diktia Sani Kisna Anita
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.116

Abstract

Gonorrhea, also known as gonorrhea, is a contagious infectious disease caused by the bacterium Neisseria gonorrhoeae. This disease is transmitted through sexual intercourse and bad habits of changing partners. The symptoms are generally asymptomatic. Purpose: this study aims to discuss the Characteristicsof Sufferers of Sexually Transmitted Infection Disease Due to Neisseria gonorrhoeae bacteria based on age and gender at the Mlati 1 Public Health Center in January-December 2022. Method: the method used is descriptive with a Cross Sectional Study approach.Results: based on the research data conducted, the results of reactive gonorrhea were 21 cases (28%) with the largest population age being adolescents 15-25 years with a total of 11 cases (52.3%). Then, in the adult age group 26-45 years there were 8 (38.2%) cases and the elderly age group 46-65 years with 2 (9.5%) cases. Gonorrhea sufferers were predominantly found in males with a total of 18 cases (85.7%) and in females totaling 3 (14.3%). Conclusion: Overview of STI cases with reactive gonorrhea with a total of 21 cases occurring in the 15-25 year age group of adolescents, and found in males.
LITERATUR REVIEW: GAMBARAN PEMERIKSAAN KULTUR DARAH DAN POLYMERASE CHAIN REACTION REACTION PADA PASIEN DEMAM TIFOID Nurmi - Hasbi
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.130

Abstract

Typhoid fever is a health problem in the world caused by the bacterium Salmonella typhi. The route of transmission of this disease is through oral-faeces. According to WHO it is estimated that every year around the world there are 10 to 21 million cases of typhoid fever. whereas in Indonesia in 2010-2019, the incidence rate is estimated to be around 500 per 100,000 population each year. The inability to make an early laboratory diagnosis often leads to increased morbidity and mortality in cases of typhoid fever. The S. typhi culture examination technique is the gold standard method for confirming the diagnosis of typhoid fever. Polymerase Chain Reacton (PCR) is an in vitro DNA synthesis and amplification technique. PCR technique can be used to amplify DNA segments of pathogenic bacteria millions of times in just a few hours. This study aims to determine the effectiveness of fever examination between culture and PCR techniques. This study used the literature review method which was carried out by collecting library data based on keywords, namely typhoid fever, culture, and PCR. Searching for articles through the Google Scholar and Pubmed databases. The results of the analysis of the literature review with a sensitivity statistical test were 0.001 (p<0.05) and the speficity test was 0.095 (p>0.05). There is a significant difference in terms of sensitivity and no significant difference in terms of specificity of blood culture and polymerase chain reaction (PCR) results
GAMBARAN TELUR CACING Oxyuris vermicularis PADA ANAK DI WILAYAH PESISIR PANTAI MERPATI BULUKUMBA Andi Harmawati Novriani. HS
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.132

Abstract

Helminthiasis is a public health problem in Indonesia. Oxyuriasis is an infectious disease caused by Oxyuris vermicularis (Enterobius vermicularis) or pinworms and is a non-STH (non-Soil Transmitted Helminth) intestinal worm that can move from one individual to another without the need for transmission through the soil and is widespread in children and toddlers. Objective: This study aims to determine the picture of Enterobius vermicularis worm eggs in children aged 3-8 years in coastal areas. Methods: This research is descriptive using a purposive sampling technique. The population in this study was 20 samples. The sampling method used the anal swab method which is the best method in diagnosing Oxyuris vermicularis. Worm eggs are taken with an anal swab or cellophane swab attached around the anus in the morning before the child does activities such as defecating, urinating, and bathing. Results: out of 20 samples, 3 samples were infected with Enterobius vermicularis eggs and 17 samples were negative. Enterobiasis cases in coastal areas are still high. Conclusion: Based on the results of Enterobius vermicularis worm eggs research, found in children aged 3-8 years, out of 20 samples found 3 samples were infected with Enterobius vermicularis worm eggs and 17 samples were negative Enterobius worm eggs. Where Enterobius vermicularis cases are still high in coastal areas.
PENGARUH WAKTU PENUNDAAN PEMERIKSAAN TERHADAP KADAR KOLESTEROL Prima Octafia Damhuri
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.133

Abstract

Pemeriksaan laboratorium merupakan pemeriksaan penunjuang yang dapat digunakan dalam menegakkan diagnosa suatu penyakit, oleh sebab itu hasil pemeriksaan laboratorium harus akurat dan dapat dipercaya. Seringkali pemeriksaan tidak dapat dilakukan dengan segera sehingga terjadi penundaan waktu pemeriksaan yang bervariasi. Hal ini perlu mendapat perhatian mengingat banyaknya faktor yang dapat mempengaruhi kecepatan dan ketepatan hasil pemeriksaan. Pemeriksaan kolesterol total dianalisis karena pemeriksaan tersebut merupakan salah satu pemeriksaan yang paling sering dilakuakan di laboratorium. Pemeriksaan kadar kolesterol tidak dapat segera dilakukan atau terpaksa ditunda apabila terjadi kerusakan pada alat di laboratorium, sehingga sampel harus disimpan sebelum analisis dilakukan. Beberapa faktor yang mempengaruhi peningkatan kadar kolesterol yaitu penundaan pemeriksaan, ketidak seimbangan komposisi enzim yang ada dalam serum yang akan diperiksa. Tujuan dari penelitian ini adalh untuk melihat pengeruh waktu penundaan pemeriksaan terhadap kadar kolesterol. Metode pengambilan sampel yang digunakan adalah random sampling yaitu sebanyak 9 sampel. Dari hasil penelitian yang telah dilakukan diperoleh hasil rerata kadar kolesterol yang diperiksa segera 167,11 mg/dL, yang ditunda selama 2 jam sebesar 153,22 mg/dL dan yang diperiksa setelah 4 jam adalah 131,33 mg/dL. Uji statistik menunjukkan Terdapat perbedaan yang signifikan hasil kadar kolesterol terhadap penundaan waktu pemeriksaan baik 2 jam maupun 4 jam dengan nilap p 0,002 (<0,05).
UJI CEMARAN Salmonella sp PADA MAKANAN JAJANAN SEKOLAH DASAR DI KELURAHAN TOBEK GODANG KECAMATAN TAMPAN PEKANBARU Siska Zafrida
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.135

Abstract

Makanan merupakan kebutuhan pokok bagi manusia agar bisa hidup sehat dan dapat melakukan aktifitas sehari-sehari dengan baik. Namun dari berbagai macam makanan yang dikonsumsi oleh manusia juga terdapat makanan yang menyebabkan penyakit pada manusia. Hal ini terjadi dikarenakan dalam pembuatan makanan yang dikonsumsi kurang higienis.Penelitian ini bersifat Eksperimental laboratory dengan cara kultur. Penelitian ini bertujuan untuk mengetahui ada tidaknya cemaran Salmonella sp pada jajanan makanan yang dijual dikantin SDN 165 dan SDN 176 Kelurahan Tobek Godang Kecamatan Tampan Pekanbaru. Dari 8 sampel jajanan makanan yang dibiakan pada media Selenit broth terjadi kekeruhan yang menandakan adanya pertumbuhan bakteri dan setelah dilakukan pewarnaan Gram ditemukan basil Gram negatif pada 8 sampel jajanan makanan. Hasil pertumbuhan koloni pada media kultur SS agar setelah dibiakan 37°C selama 1 x 24 jam pada 8 sampel ditemukan pertumbuhan koloni dengan ciri-ciri koloni bulat, putih. Berdasarkan hasil reaksi biokimia dapat disimpulkan bahwa sampel jajanan makanan di kantin SDN 165 dan SDN 176 Kelurahan Tobek Godang Kecamatan Tampan Pekanbaru tidak ditemukan Salmonella sp dengan persentasi cemaran sebesar 0%.
IDENTIFIKASI TELUR CACING PADA KUKU ANAK-ANAK DI TPA MUARA FAJAR KOTA PEKANBARU Weni Mulyani
Jurnal Sains dan Teknologi Laboratorium Medik Vol 9 No 1 (2023): April (2023)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v9i1.136

Abstract

Kecacingan merupakan salah satu penyakit yang masih menjadi masalah kesehatan bagi masyarakat Indonesia hingga saat ini. Penyakit infeksi kecacingan bisa di tularkan melalui kaki, tangan atau kuku yang terkontaminasi tanah yang mengandung telur cacing, lalu masuk ke mulut bersama makanan. penyakit infeksi kecacingan yang menyerang usus terdiri atas 3 phylum yaitu nematoda, cestoda dan trematoda. Salah satu daerah di Pekanbaru yang rentan terhadap kecacingan adalah Tempat Pembuangan Akhir (TPA) Muara Fajar Kota Pekanbaru, wilayah ini sangat lembab dan kumuh. TPA Muara Fajar selain menjadi tempat pembuangan sampah di Kota Pekanbaru ternyata juga merupakan kawasan sumber aktivitas ekonomi bagi para pemulung. Tidak hanya pemulung usia dewasa, tetapi juga pemulung usia anak-anak. Anak-anak yang tinggal disekitar daerah ini memiliki risiko lebih tinggi terinfeksi kecacingan, karena TPA dijadikan sebagai tempat bermain dan bekerja bagi anak-anak yang tinggal di wilayah tersebut. Penelitian ini bertujuan untuk mengidentifikasi telur cacing pada kuku tangan dan kuku kaki anak-anak di TPA Muara Fajar Kota Pekanbaru. Hasil penelitian ditemukan telur cacing Ascaris lumbricoides pada kuku tangan sebanyak 4 sampel, pada kuku kaki 4 sampel dan telur Taenia sp pada kuku tangan sebanyak 2 sampel, kuku kaki 1 sampel. Berdasarkan penelitian disimpulkan bahwa terdapat 2 jenis telur cacing yang ditemukan pada kuku tangan dan kuku kaki anak-anak di TPA Muara Fajar Pekanbaru yaitu telur cacing Ascaris lumbricoides dan Taenia sp.

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