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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "Vol 1 No 2 (1995): December 1995" : 5 Documents clear
PENGARUH PENAMBAHAN ENZIM DAN WAKTU INKUBASI TERHADAP JUMLAH PROTOPLAS MESOFIL DAUN ANGGREK Dendrobium Sp Edu Setiti Wida Utami
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 2 (1995): December 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.652 KB) | DOI: 10.23869/174

Abstract

This research was done in order to study of effect the supplementation of enzyme and incubation time for the protoplast quality leaf mesophyll Dendrobium sp. Protoplast can be used for culture protoplast, for somatic cross, biology research, and material for genetic manipulation. This research to make use of material was Dendrobium sp Orchid. Explant that to used was leaf mesophyll Dendrobium sp. For the protoplast isolation to used enzyme combination cellulase and maserozym (Onozuka R-10). Yakult JONSHA Co., Ltd., (Japan) with concentration cellulase : maserozym is (0.5; 0.05; 0.75; 0.075; 0; 0.1). leaf mesophyll Dendrobium sp. to get a soak in enzyme solution with incubation time 12-13 hours and 14-1 hours.the result shown that enzyme combination selulase and maserozym can be used for protoplast isolation leaf mesophyll Dendrobium sp Orchid. The best enzyme concentration to fit for isolation protoplast leaf mesophyll Dendrobium sp to be selulase 0.75% and maserozym 0.075% with incubation time 14-15 hours.
APLIKASI BEBERAPA INOKULAN Rhizobium DAN INTERVAL PEMOTONGAN HIJAUAN PAKAN TERNAK Stylosanthes guyanensus TERHADAP KANDUNGAN NITROGEN TANAH DI LAHAN KERING Kusriningrum Rochiman
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 2 (1995): December 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.339 KB) | DOI: 10.23869/175

Abstract

This research on discovering compatible Rhizobium inoculum for Stylosanthes guyanensis, the influence of defoliation interval on forage and the influence of interaction between inoculum and defoliation interval on nitrogen content in dry land had been conducted in Ranuklindungan Village, Grati, Pasuruan since July 1993 until November 1994. This experiment used factorial with randomized block design, with two factor (Rhizobium inoculum : without inoculum, inoculum of Stylosanthes guyanensis planted soil, legin of Leguminosae Cover Crops (Calopogonium mucunoides), Legin of Glycine max ; and defoliation interval ; without interval defoliation, 40 days defoliation interval, 60 days defoliation interval, and three replications. The inoculation which was manifested on nodule formation influences the nitrogen content in soil on 12 month old. Stylosanthes guyanensis with the best result on inoculation of Stylosanthes guyanensis planted soil. The defoliation interval doesn't influence the nitrogen content in soil. There are no interaction between inoculum and defoliation interval on nitrogen content in soil. The 4-8 month-old Stylosanthes guyanensis need supplementary nitrogen of soil media, the 8-12 month-old ones can increase nitrogen content of soil media up to 17.39 % by inoculation of Stylosanthes guyanensis planted soil and up to 12.58 % by 60 days defoliation interval.
FRAKSINASI AMILASE DARI Endomycopsis fibuligera ITBRcc64 DENGAN METODE PRESIPITASI AMONIUM SULFAT Afaf Baktir
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 2 (1995): December 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.749 KB) | DOI: 10.23869/176

Abstract

Endomycopsis fibuligera ITB.R.cc.64 produced high extracellular amylase activity. It showed two kinds of amylase activities, those are liquefication and saccharification. The aim of this research are to separate each kind of amylase by fractionation with ammonium sulfate and to test the specific activity of each fraction. It was done three fraction ammonium sulfate: 0-40 %, 40-60 %, and 60-80%. This fraction has the strong liquefication activity of 20.847 U/mg protein, the second fraction has the strong saccharification activity of 18.903 U.mg protein and the weak liquefication activity of 1.756 U/mg protein.
PENGARUH PEMANGKASAN DAN PENAMBAHAN GIBERELIN (GA3) TERHADAP LAMA WAKTU PEMBENTUKAN TUNAS DAN KUNCUP BUNGA TANAMAN MAWAR (Rosa sp) Listijani Suhargo; Y. Sri Wulan Manuhara
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 2 (1995): December 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.375 KB) | DOI: 10.23869/177

Abstract

The effect of cutting and adding gibberellin on the duration of stem and flower bud forming of Rosa sp had been done. This research purpose to shorthen the duration of stem and flower bud forming. The research sample were Rose plant with yellow colour and the same length and number of branches. The cutting was done on 0 cm, 5 cm, and 10 cm from the apex. In this research, we used gibberellins with 50 ppm and 100 ppm concentrations and as a control we used aquadest. The result showed that the best treatment to shorthen the duration of stem bud forming were the cutting 10 cm from the apex and with adding of gibberellin on 100 ppm concentration. And the best treatment to shorthen the duration of flower bud forming were with the cutting on 10 cm from the apex and with adding of gibberellins on 50 ppm concentration.
STUDI MEKANISME KINETIKA REAKSI PERTUKARAN ION FE(III) ANTARA DUA JENIS LIGAN SIDEROPHORE BAKTERI (DESFERRIFERRIOXAMINE B DAN AZOVERDINE) DENGAN LIGAN EDTA Handoko Darmokoesoemo; A.M. Albrecht Gary
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 2 (1995): December 1995
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.885 KB) | DOI: 10.23869/178

Abstract

The kinetics of iron exchange and iron removal from two siderophore complex with use of ethylenediaminetetraacetic acid (EDTA) have been examined, using UV-Vis spectrophotometric method. Iron exchange between the ferric complexes of desferriferrioxamine B (siderophore produced by microorganism Azomonas macrocy-togenes) with EDTA was extremely slow under conditions at pH 7.4 and 25oC. Furthermore, pH dependence studies demonstrated that the exchange rate was accelerated as a function of increasing hydrogen ion concentration. Kinetics of iron removal from ferrioxamine B and the ferric complexe of azoverdine with excess of EDTA, show firt-order dependence on the concentration of the iron complex and hydrogen ion at 25oC with first-order rate constant of the dissociation of a ternary complex formed of (16 ±0.4) x 10-3s-1 and (1.3 ±0.2) x 10-2s-1 respectivy at pH region between 3.67 and 5.23. variation of pseudo first order rate constant kobs with hydrogen ion concentration shows a saturation kinetics at high constant ligand EDTA concentration (3.0 x 10-2 M) and at low pH region (3.67). the result were interpreted in terms of three-step mechanism, involving : (1) protonation of the ferrioxamine B comples and the ferric comples of azoverdine and (2) subsequent biomolecular reaction with EDTA.

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