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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss.v26i2.xxx
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 2 Documents
 1 
Search results for , issue "Vol. 20, No. 4" : 2 Documents clear
Antifungal Activity of (+)-2,2’-Epicytoskyrin A and Its Membrane-Disruptive Action Wulansari, Dewi; Praptiwi,; Julistiono, Heddy; Nurkanto, Arif; Agusta, Andria
Makara Journal of Science Vol. 20, No. 4
Publisher : UI Scholars Hub

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Abstract

(+)-2,2’-Epicytoskyrin A, a bis-anthraquinone isolated from fungal endophyte Diaporthe sp. GNBP-10 associated with Uncaria gambir Roxb., was investigated for its antifungal activity. The broth microdilution method was used to determine the minimum inhibitory concentration (MIC) against 22 yeast strains and three filamentous fungi. The MICs of (+)-2,2’-epicytoskyrin A ranged from 16 to 128 µg/mL, which exhibited lower activity than the antifungal nystatin. A study of the mechanism of action revealed similar effects of (+)-2,2’-epicytoskyrin A and nystatin on Candida tropicalis at their MICs (16 and 8 µg/mL, respectively) and 2 times of the MIC. Both compounds caused cytoplasmic material and ion leakages on fungal cell, which were characterized by an increase in absorbance at 260 nm and 280 nm as well as Ca2+ and K+ ion concentrations. The morphology of the fungal cells after (+)-2,2’-epicytoskyrin A treatment was observed under a scanning electron microscope. The control cells, which were not treated with either (+)-2,2’-epicytoskyrin A or nystatin, showed a smooth surface, while the cells treated with either (+)-2,2’-epicytoskyrin A or nystatin shrank and displayed a donut-like shape. More shrinkage was observed in the 2 times MIC concentration and even more in the cells exposed to nystatin. The action of (+)-2,2’-epicytoskyrin A was proposed through membrane disruption.
A New Benzoyl Compound Isolated from the Endophytic Fungi of Kandis Gajah (Garcinia griffithii) and Asam Kandis (Garcinia cowa) Elfita,; Munawar,; Muharni,; Pratiwi, Gusti; Rahmadania,
Makara Journal of Science Vol. 20, No. 4
Publisher : UI Scholars Hub

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Abstract

Garcinia griffithii and Garcinia cowa belong to the genus Garcinia. The genus Garcinia has been known to be a rich source of secondary metabolites, such as xanthones, benzophenones, flavonoids, steroids, terpenoids, and other phenolic derivatives. Previous investigations of endophytic fungi from G. griffithii revealed the presence of three compounds not found in the host. In order to the continue the phytochemical work on endophytic fungi of G. griffithii, the constituent of the endophytic fungi of G. griffithii was re-examined. In this study, a benzoyl compound similar to that found in the endophytic fungus of G. cowa was observed. The same benzoyl compound was also isolated from the endophytic fungus Acremonium sp of G. griffithii and Aspergillus sp of G. cowa with cultivation of eight weeks in static conditions at room temperature. The culture medium was partitioned using ethyl acetate and evaporated to obtain the concentrated extract. Isolation of compounds was performed using the chromatography method. The chemical structure was proposed on the basis of spectroscopic data, including ultraviolet (UV), infrared (IR), mass spectrometry (MS), proton nuclear magnetic resonance ( 1H-NMR), carbon nuclear magnetic resonance ( 13C-NMR), heteronuclear single-quantum correlation spectroscopy (HSQC), heteronuclear multiple-bond correlation spectroscopy (HMBC), and correlation spectroscopy (COSY).

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