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INDONESIA
Indonesian Biodiversity Journal
Published by Universitas Negeri Manado
ISSN : 27222659     EISSN : -     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Biodiversity Journal (IBJ) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biological science fields such as biodiversity, biopharmaca, DNA barcoding and molecullar biology, ecology, physiology, behavior, and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by International Biodiversity Journal (IBJ).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 3 Documents
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Search results for , issue "Vol. 1 No. 2 (2020): Agustus 2020" : 3 Documents clear
HEMATOLOGY PARAMETERS OF THE WHITE RAT (Rattus norvegicus) IN VARIOUS PHYSIOLOGICAL CONDITIONS OF REPRODUCTION Sumiaty Aiba; Livana Dethris Rawung
Indonesian Biodiversity Journal Vol. 1 No. 2 (2020): Agustus 2020
Publisher : Universitas Negeri Manado

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Abstract

Rattus norvegicus is one of the experimental animals that is widely used in research. Knowing the hematological status of R. norvegicus rats during reproduction is the aim of this study. In this study was used 12 female rats were sexually mature. Data were collected at the rats that were not yet pregnant (T0); 10 days pregnant mouse (T10); 20 days pregnant mouse (T20); 7-day lactated rat (L7); and 20-day lactating mice (L20). This study shows that during pregnancy and breastfeeding there is an increase in the level of erythrocytes in the blood. During pregnancy, there is a decrease in hemoglobin levels and higher during lactation. The hematocrit value during lactation shows a higher value than in the non-pregnant and pregnant states.
OVARY AND UTERINE RNA/DNA RATIO IN VARIOUS REPRODUCTIVE CONDITIONS OF FEMALE WHITE RAT (Rattus norvegicus) Livana Dethris Rawung; Kasiyati
Indonesian Biodiversity Journal Vol. 1 No. 2 (2020): Agustus 2020
Publisher : Universitas Negeri Manado

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Abstract

RNA / DNA ratio is a parameter that shows the activity of protein synthesis that is taking place in a cell. In mammals, during the reproductive period, the ovaries and uterus are the organs that play an active role. The purpose of this study was to know the activity of protein synthesis that occurs in the ovaries and uterus in female white rats (Rattus norvegicus) in various physiological conditions during reproduction. In this study, female rats that were sexually mature were used. Data were collected on rats with physiological status not yet pregnant (T0); pregnancy 10 and 20 days (T10, T10), and lactation 7, 10, and 20 days (L7, L10, L20). The results showed that there was a decrease in the value of the ovary RNA / DNA ratio during pregnancy and lactation. In contrast, the uterine RNA / DNA ratio increases during pregnancy and lactation. Based on the results of the study, it can be concluded that the increase in the uterine RNA: DNA ratio that occurs during pregnancy is because it is used for the synthesis of various structural and functional components for fetal growth and development
MORPHOLOGY OF Patchouli OIL MICROCACULES (Pogostemon cablin Benth) WHICH IS ENKAPSULATED USING CHITOSAN Kleriyani Mayan; Arrijani; Emma Moko
Indonesian Biodiversity Journal Vol. 1 No. 2 (2020): Agustus 2020
Publisher : Universitas Negeri Manado

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Abstract

Patchouli oil (Pogostemon calbin Benth) has economic benefits which are widely used as raw material for the pharmaceutical, perfume and food industries. Although widely used in various fields, patchouli oil or essential oils are generally young to evaporate, are susceptible to high temperatures and humidity, so we need a way / solution to overcome this, one way is encapsulation. Encapsulation is a technique to protect the core material which was originally liquid from becoming a solid / powder form and can protect the core material from losing its taste and aroma. Coating is a material that can mix with the core material, is not reactive to the core material and can form a layer around the core material. Chitosan is a good coating for essential oil encapsulation. This study aims to determine the particle size and microcapsule characteristics of patchouli oil (P.calbin Benth) using chitosan coating. The microcapsules were made using a freeze dryer with the freeze drying method. The dried samples were analyzed for particle size, morphological shape and content using SEM (Scanning Electron Microscopy). The results showed that the samples with a coating material composition of 1:2 were completely dry freeze drying. The average particle size <3.5 µm with a magnification of 10,000x with round morphology and various shapes. Analysis of the chemical content in the sample showed the similarity of the main compounds in patchouli essential oil, namely α-guaiene, azulene, α/β/ γ-patchoulene, seychellene, and α-himachalene and there was the addition of carbon (C) 76.07% and oxygen. (O) 16.46% originating from coating material

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