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Nonny Manampiring
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nonny@unima.ac.id
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nonny@unima.ac.id
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Jl, Kampus Unima, Tonsaru, Minahasa - North Sulawesi. Indonesia 95632
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Kab. minahasa,
Sulawesi utara
INDONESIA
Indonesian Biodiversity Journal
Published by Universitas Negeri Manado
ISSN : 27222659     EISSN : -     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Biodiversity Journal (IBJ) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biological science fields such as biodiversity, biopharmaca, DNA barcoding and molecullar biology, ecology, physiology, behavior, and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by International Biodiversity Journal (IBJ).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 49 Documents
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ISOLATION AND IDENTIFICATION OF ENTOMOPATHOGENIC ON CROSIDOLOMIA BINOTALIS [ZELLER] (LEPIDOPTERA : PHYRALIDAE) USING 16S rRNA GENE: ISOLATION AND IDENTIFICATION OF ENTOMOPATHOGENIC ON CROSIDOLOMIA BINOTALIS [ZELLER] (LEPIDOPTERA : PHYRALIDAE) USING 16S rRNA GENE Carolin Manuahe
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

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Abstract

The aims of this reserach were to isolating and to identifying bacteria entomopathogenic on caterpillar pests in cabbage. The study was conducted in three phases. The first stage isolation of bacteria from cabbage caterpillar pests, the second stage of microscopic characterization of bacterial isolates and the third phase molecular identification of bacteria isolates using 16S RNA gene. The results were obtained five bacterial isolates of caterpillar pests in cabbage which 4 isolates shaped cocus, three isolates of gram positive and gram negative two isolates. All of isolates were able to degrade D-glucose. Isolates were translucent white color, white yellowish and brownish white. Generally isolates are not motile dan growth in the media to the surface. Results of DNA extraction and amplification of 16S RNA gene, showing the purity and concentration of DNA is good, confirmed by electrophoresis, amplicons formed band at 1500 bp according to the characteristics of the 16 S RNA gene
Characteristics Of Partial Mithchondrial Cytochrome Oxidase Sub Unit 1 Gene Of Honey Bee Apis dorsata Binghami [Hymenoptera: Apidae] From Minahasa, North Sulawesi, Indonesia: Characteristics Of Partial Mithchondrial Cytochrome Oxidase Sub Unit 1 Gene Of Honey Bee Apis dorsata Binghami [Hymenoptera: Apidae] From Minahasa, North Sulawesi, Indonesia Yermia Semuel Mokosuli; Revolson Alexius Mege; Jantje Pelealu; Max Tulung
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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Abstract

The aims of this study were to characterize the mitochondrial cytochrome oxidase subunit 1 gene (CO1) of Apis dorsata from Minahasa, North Sulawesi. Apis dorsata Binghami used in this study was obtained from the natural colony in Klabat forest, Airmadi forest and Kombi forest. From each native bee colony, ten workers were taken (n = 30). Femur hind legs used for subsequent DNA extraction were amplified by PCR, underwent electrophoresis and then sequenced. CO1 sequences then were matched with BLAST to obtain sequences from the NCBI that have high levels of homology (>90%). The results showed that the universal primer LCO -1490 and HCO - 2198 success amplify the gene CO1 Apis dorsata of Minahasa. Based on the phylogeny tree, Apis dorsata derived from forest Airmadidi and forest Kombi have the same CO1 sequences but have had differences with Apis dorsata from forests Klabat. BLAST analysis results showed that the partial mitochondrial CO1 sequences Apis dorsata of Minahasa has the highest rate of 94 % similarity with similar sequences recorded in the NCBI gene bank.
Insect Diversity In Forest Of Mount Klabat, Kauditan Subdistrict North Minahasa Regency: Insect Diversity In Forest Of Mount Klabat, Kauditan Subdistrict North Minahasa Regency Iwan Sixtus Umboh
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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Abstract

This study aims to assess The population and insect diversity in the Forest of Klabat. Sequences of this study consisted of determining the location of the sample, the sample unit, and forest. This research was conducted using survey methods, identification, and analysis data. The resulting study showed that insect populations and diversity obtained from the forest of Klabat is a total of 190 individuals consisting of 29 orders and 23 families, with the amount of each individual as follows: 34 Diptera, Hymenoptera 25, 21 Coleoptera, Lepidoptera 18, .and Orthoptera 5. The population the highest of the forest that is on the order Diptera Family Lauxamidae. Insect Diversity in the forest of Klabat at altitude 301 -400 masl is H. =4,76, and in the forest of Klabat at altitude 401 – 500 masl is H. = 2,31.
Effectiveness of Curcumin Supplementation in feed on liver productivity and reproductive performance of female carp Cyprinus carpio L. : Effectiveness of Curcumin Supplementation in feed on liver productivity and reproductive performance of female carp Cyprinus carpio L. Livana Dethris Rawung; Jacson Saruan
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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Abstract

The availability of quality seeds, on time with large amounts is a major problem in freshwater aquaculture. Supplementation of curcumin in female carp aims to increase the reproductive productivity of fish through increased activity of the liver in the synthesis of vitellogenin, a precursor of egg yolk, that play a role in the growth and development of eggs until larvae. This study used 25 female carp, which supplemented with curcuminat several doses: 0; 2.5; 5; 10; and 20 g of curcumin/kg of feed. The collected data was analyzed using a completely randomized design (CRD). The results showed that supplementation of curcumin female carp significantly (p<0.05) affected the malondialdehyde(MDA) concentration of liver, estradiol hormone in the blood, gonadosomatic index, relative fecundity, and larval survival, but did not significantly (p>0.05) affect the superoxide dismutase (SOD), vitellogenin concentration in blood and egg diameter. The study showed that the supplementation of curcumin prevents liver damage showed by a decreased level of liver MDA. The supplementation of curcumin with a dose of 5 g/kg of feed optimizes the productivity and reproductive performance of carp by increasing the production of vitellogenin, the diameter of egg, and the number of survival larva through optimizing the performance of the fish liver.
Aedes Mosquito Population density of Dengue Fever Vectors in The Area of Pineleng Minahasa: Aedes Mosquito Population density of Dengue Fever Vectors in The Area of Pineleng Minahasa Carolin Manuahe; Yermia Semuel Mokosuli; Ferny Margo Tumbel
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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Abstract

Aedes mosquitoes have the behavior of sucking blood repeatedly (multiple bites) in one gonotrophic cycle, these mosquitoes are also very effective at transmitting the virus to humans (WHO 2004). Kawada et al. (2007) reported that the foraging activity of A. albopictus was less than 0.1 times compared to A. aegypti under laboratory conditions, this result is consistent with the tendency to bite both species in nature. The target-attack frequency (foraging activity) in A. aegypti female mosquitoes in unfed conditions is 30 times greater than that of A. albopictus mosquitoes in several laboratory treatments (Kawada, et al. 2007). Aedes sp is a deadly infectious vector between another dengue hemorrhagic fever (DHF) and chikungunya. Pineleng is a marginal/suburban area of Station 1. Recent studies have reported changes in habitat patterns of Aedes sp, especially Aedes albopictus and Aedes aegypti . The aim of this study is to characterize the population density of the Aagoan sp. Imago based on the time distribution and altitude of the sea surface. The population density characteristics of the Aedes spagoed imago captured inside and outside the room based on the time distribution in North Sulawesi. The stages of the study consisted of larvae surveying, larval extraction and analysis, and imago extraction and analysis. The results showed that the high population of Ae aegypti was at 8:00 a.m. to 10:00 p.m. in the morning and 14:00 to 16:00 p.m. in the afternoon before the afternoon. in the morning before noon and at 14:00 to 18:00 in the afternoon before evening.
Hepatoprotective Activities Of Polar And Non Polar Extract Kembang Sepatu Flower (Hibiscus rosasinensis L.): Hepatoprotective Activities Of Polar And Non Polar Extract Kembang Sepatu Flower (Hibiscus rosasinensis L.) Adil Ellen Hettie; Nonny Manampiring
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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This study aims to obtain the dominant phytochemical group in polar and non-polar extracts of hibiscus flowers and obtain data on hepatoprotective activity of polar and non-polar extracts of hibiscus flowers on male white rats induced by high doses of paracetamol. This study consists of the stages of extraction, stages phytochemical analysis and test phase of hepatoprotective activity using rat test animals. Treat done is negative control (K -). This group was only given solvents (CMC Na 0.5%) 1ml / head / day for 28 days and was not given heat stress treatment. H0. This group was given solvents (CMC Na 0.5%) 1ml / head / day for 28 days, after which they were given heat stress treatment for eight days. H1 This group was given a polar extract of Hibiscus rosasinensis red flowers at a dose of 0.075g mg / 200gramBB / day in a 0.5% CMC suspension of 1 ml for 14 days, and after that was given heat stress treatment for eight days. H2. This group was given a non-polar extract of Hibiscus rosasinensis red flowers at a dose of 0.075mg / 200gramBB / day in a 1% CMC suspension of 1 ml for 14 days, and after that was given heat stress treatment for eight days. The measured parameters were the content of SGPT and SGOT . The results showed that the polar extract and non-polar extract Hibiscus rosasinensis L. red variety were able to maintain levels of the SGOT and SGPT enzymes in white rats (Rattus norvegicus) induced by high doses of paracetamol, as in the negative control group (K-).
Nutmeg (Myristica fragrans) Of North Minahasa Molecular Entication Based on chloroplast DNA of matK gene Fanny Nella Nanlohy; Yermia Semuel Mokosuli
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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Plant Nutmeg (Myristica fragrans) is an endemic plant in Indonesia originating from Banda Maluku Province. Nutmegs have long been known to people as spices. The nutmeg plants have been cultivated in North Sulawesi for a long time. This research has been conducted to obtain the position of species and the reconstruction of the phylogenetic tree of nutmeg plant (Myristica fragrans) from North Minahasa based on chloroplast DNA of matK gene. This research uses a descriptive method with a laboratory experiment. Total DNA was obtained by leaf tissue extraction and purification. The matK gene was amplified using the PCR method and visualized with automatic electrophoresis, sequencing was performed at FIRST BASE Singapore. The result of the analysis of the gene sequence of North Min Minerals using Barcode Of Life Database (BOLD) Systems shows that Pala Wori and Pala Patokaan have 99% similarity with Myristica maingayi and Myristica glubosa. While the reconstruction of the phylogeny tree shows the North Minahasa Pala has the closest resemblance to Myristica fatua.
Description Of Local Pigs Histopathology in Small Islands of North Sulawesi Revolson Alexius Mege; Mery Friska Montolalu; Rolef Rumondor; Nonny Manampiring
Indonesian Biodiversity Journal Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Negeri Manado

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Some areas in Indonesia have local pigs with their own special characteristics. One of them is North Sulawesi which has local pigs that are maintained by the population with a traditional system and some live wild. The liver is the largest gland that has an important function for living things. This study aims to determine the histology of native pig liver from small islands in North Sulawesi. The liver is histopathologically processed with hematoxylin-eosin staining and then the preparation is analyzed descriptively. The results showed a histological picture in the Buhias and Bunaken areas showing necrosis and widening of the sinusoid while histological features in the Kahuku, Bangka, and Nain regions showed that the liver had severe steatosis and cirrhosis of the liver.
HEMATOLOGY PARAMETERS OF THE WHITE RAT (Rattus norvegicus) IN VARIOUS PHYSIOLOGICAL CONDITIONS OF REPRODUCTION Sumiaty Aiba; Livana Dethris Rawung
Indonesian Biodiversity Journal Vol. 1 No. 2 (2020): Agustus 2020
Publisher : Universitas Negeri Manado

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Rattus norvegicus is one of the experimental animals that is widely used in research. Knowing the hematological status of R. norvegicus rats during reproduction is the aim of this study. In this study was used 12 female rats were sexually mature. Data were collected at the rats that were not yet pregnant (T0); 10 days pregnant mouse (T10); 20 days pregnant mouse (T20); 7-day lactated rat (L7); and 20-day lactating mice (L20). This study shows that during pregnancy and breastfeeding there is an increase in the level of erythrocytes in the blood. During pregnancy, there is a decrease in hemoglobin levels and higher during lactation. The hematocrit value during lactation shows a higher value than in the non-pregnant and pregnant states.
OVARY AND UTERINE RNA/DNA RATIO IN VARIOUS REPRODUCTIVE CONDITIONS OF FEMALE WHITE RAT (Rattus norvegicus) Livana Dethris Rawung; Kasiyati
Indonesian Biodiversity Journal Vol. 1 No. 2 (2020): Agustus 2020
Publisher : Universitas Negeri Manado

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RNA / DNA ratio is a parameter that shows the activity of protein synthesis that is taking place in a cell. In mammals, during the reproductive period, the ovaries and uterus are the organs that play an active role. The purpose of this study was to know the activity of protein synthesis that occurs in the ovaries and uterus in female white rats (Rattus norvegicus) in various physiological conditions during reproduction. In this study, female rats that were sexually mature were used. Data were collected on rats with physiological status not yet pregnant (T0); pregnancy 10 and 20 days (T10, T10), and lactation 7, 10, and 20 days (L7, L10, L20). The results showed that there was a decrease in the value of the ovary RNA / DNA ratio during pregnancy and lactation. In contrast, the uterine RNA / DNA ratio increases during pregnancy and lactation. Based on the results of the study, it can be concluded that the increase in the uterine RNA: DNA ratio that occurs during pregnancy is because it is used for the synthesis of various structural and functional components for fetal growth and development

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