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Journal of Biodiversity and Biotechnology
Published by Universitas Sebelas Maret
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 6 Documents
 1 
Search results for , issue "Vol 3, No 1 (2023)" : 6 Documents clear
Role of ZA and KCl Fertilizer on Growth and Yield of Red Ginger (Zingiber officinale var. Rubrum) Supriyono Supriyono; A Y Darmawan; S Nyoto; M T S Budiastuti
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.74436

Abstract

Red ginger is one of the commodities much needed by the people of Indonesia. The increase in public demand has not been matched by an increase in red ginger production. Efforts to increase red ginger production can be done with effective fertilization. This study aims to determine the appropriate dose of ZA+KCl fertilization for good growth and yield of red ginger. This research was conducted in March-August 2021 in the experimental area of Pelem, Wonorejo, Jatiyoso, Karanganyar, Central Java with coordinates 7°43'24.7”S 111°05'31.2”E with an altitude of 762  ASL. This study used a Randomized Completed Block Design (RCBD) with one factor and 4 treatments, namely D0 (control), D1 (ZA 225 kg.ha-1+KCl 50kg.ha-1), D2 (ZA 450 kg.ha-1+ KCl 100 kg.ha-1) and D3 (ZA 675 kg.ha-1+KCl 150 kg.ha-1) were repeated 6 times each. The results showed that the application of several doses of fertilizer did not increase plant height, number of leaves, dry weight of straw but could increase the number of tillers per clump by 37.11% and the number of tillers per plot by 27.10%. The results of regression analysis showed that D2 had a trend to increase fresh weight and storage weight of rhizomes with an average of 1.9 t.ha-1 and 1.5 t.ha-1. This research needs to be further developed by improving environmental influences to increase the growth and yield of red ginger.
EFFECT OF ALUMINIUM STRESS ON GERMINATION, GROWTH AND PHOTOSYNTHETIC PIGMENTS OF Amaranthus hybridus Musyimi Mutisya David
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.75211

Abstract

Amaranthus hybridus is widely distributed and used as a food plant in many parts of the world. Aluminium toxicity in the soil is one environmental stress that limits crop growth and production in acid soils. However, the level of aluminium stress tolerance depends on the plant species or genotype.  A study was conducted at Maseno University, Kenya on the effects of aluminium stress on germination, growth and leaf photosynthetic pigments of  Amaranthus hybridus seedlings. Ten uniform seeds of Amaranthus hybridus weretreated in sodium hypochlorite solution and were placed on dried petri dishes lined with Whatsman No. 1 filter papers.  Filter papers were then moistened with 5ml of  nutrient solution containing either 0 mM (distilled water) or (2, 4, 6, 8 and 10 mM) (Al toxicity) AlCl3·6H2O replicated three times. The germination was assessed for five days to ascertain the number of seeds that germinated over time.  Five litre pots were filled with top soil from Maseno University’s garden. Five seeds of Amaranthus hybridus were sown in each of the pots and were irrigated every day with 250 ml of tap water per pot for two weeks. After two weeks the seedlings were thinned to 2 plants per pot and treatments initiated. Seedlings were irrigated daily with nutrient solution containing 0 mM (control – tap water) or (2, 4, 6, 8 and 10 mM) (Al toxicity) AlCl3·6H2O in triplicates arranged in a completely randomized design in a green house. Data on shoot height, leaf area and number of leaves, shoot and root fresh and dry weights, chlorophyll a, chlrophyl b, total chlorophyll and carotenoid contents were determined at the end of the experiment. Data collected was subjected to analysis of variance (ANOVA) and treatment means separated and compared using Least Significance Difference (LSD). Germination percentage of Amaranthus hybridus was significantly reduced by the aluminiumsolution stress as compared to control treatment. Growth and photosynthetic pigments parameters were significantly reduced by the aluminium stress.The results of this study have clearly shown that  Amaranthus hybridus speciesis very sensitive to aluminium toxicity.
Effect of gamma irradiation on total phenolic contents and antioxidant activities of methanolic extract of java plum seeds Rohadi Rohadi; Iswoyo Iswoyo; Dewi Larasati; Sugili Putra
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.71721

Abstract

The synthetic antioxidant butylated hydroxyanisole (BHA) has been widely added to lipids and food products as a preservative due to inhibiting lipid oxidation. However, concerns have arisen about the potential health impacts of this synthetic antioxidant on consumers. Therefore, there is a need for an alternative substitute for BHA that is safe for human health. The methanolic extract of Java Plum seeds (MEJS) contains a diverse group of phenolic compounds and has the potential to serve as a natural antioxidant. The antioxidant must remain stable during food processing, such as irradiation. This research aimed to determine the effect of γ-irradiation on the total phenolic content and antioxidant activity of MEJS. When γ-irradiation was applied to MEJS at doses up to 12.5 kGy, there was a slight decrease in total phenolic and tannin content but a significant increase in total flavonoid content. This increase in total flavonoids led to a rise in both free radical scavenging and reducing power activities
Isolation And Identification of Arbuscula Mycorrhiza (Ma) In Plant Rhizosphere in Greenbelt Area of PT. Semen Indonesia Tbk Tuban Avivi Nur Aina; Supiana Dian Nurtjahyani; Sriwulan Sriwulan; Eko Purnomo
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.72038

Abstract

The Green Belt area is a buffer area in an industrial area that functions as a barrier or separates the mining area from other regions, such as residential areas or community agriculture. The Green belt area consists of various plant vegetation that absorbs carbon dioxide and air conditioning, preventing sedimentation and erosion in the mining area. Arbuscular mycorrhiza has the potential to live in the green belt area by forming a mutualism symbiosis with plants or vegetation in the area. This study aimed to determine arbuscular mycorrhizae found in the Greenbelt area of PT Semen Indonesia Tbk Tuban. Sampling used purposive sampling techniques at three sampling stations, namely rhizosphere eucalyptus (Malaleuca cajuputi), trembesi (Samanea saman), and sawo (Manilkara zapota). The study was conducted in November 2021 through three stages: sampling, sample isolation, and species identification. The results showed seven types of arbuscular mycorrhiza from five different genera, namely the genus Glomus, Acaulospora, Gigaspora, Racocetra, and Archaeospora. The most found species is the genus Glomus. This research contributes as a source of literacy and information about the types of AMF found in PT Semen Indonesia's Greenbelt area, which can be used as biological fertilizer, support plant growth, help reclaim mining areas, and protect plants from drought pressure in mining areas.
Risk factors for H9N2 contamination in live bird markets of the Greater Jakarta, Indonesia Rahma Hani Aisah; Bambang Sumiarto; Hendra Wibawa; Widagdo Sri Nugroho; Aprizal Panus; Sodirun Sodirun
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.74263

Abstract

Low Pathogenicity Avian Influenza virus subtype H9N2 causes decreased egg production and two of the four defined H9N2 poultry lineages (G1 and Y280) have been associated with human infections. The high positive test results for the H9N2 virus in the LBMs of the Greater Jakarta area in 2021 can be a source of transmission for the surrounding area. This study aims to determine the prevalence and risk factors that play a role in the H9N2 contamination in the LBMs to obtain a multivariate analysis model. A cross-sectional study was conducted on 87 vendors and 124 broiler chickens from 44 LBMs randomly selected in Tangerang City, North Jakarta, and Bekasi City. Samples were taken as equipment swabs at the stall of poultry carcass vendors, tracheal swabs, and cloacal swabs of broiler chicken. The test used was Real-Time Reverse Transcription Polymerase Chain Reaction. Data analysis was performed using univariate, bivariate, and multivariate analysis with logistic regression. The prevalence of H9N2 virus contamination at the LBM level in Greater Jakarta in 2022 was 77.27% (95% CI, α 7%), while the prevalence at the vendor level was 54.02% (95% CI, α 5%). The risk factors for H9N2 virus contamination with a significant p-value<0.05 include the positive results on equipment swab samples, positive results on both equipment and broiler swabs, and the use of carcass sink.  The multivariate analysis model of H9N2 virus contamination at the vendor level was Logit P (H9N2=1 | x) = 0.29924 - 2.42176PPE + 1.58691carcass sink. The risk factor contributing to increase H9N2 contamination is using carcass sinks, while PPE is the one that reduces H9N2 contamination.
Epidemiology Study of Brucellosis in Polewali Mandar District, Indonesia Fitri Amaliah; Bambang Sumiarto; Widagdo Sri Nugroho; Tri Untari; Dinar Hadi Wahyu Hartawan; Agustia Agustia
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.74264

Abstract

This research aims to determine the prevalence rate of brucellosis in the Polman District, examine the risk factors associated with seropositive diagnoses of brucellosis, and also to determine the brucellosis case model at the farm level. This research was carried out through a cross-sectional study and obtained a sample size of 395 serum. Samples were tested through the RBT and CFT. Identification of risk factors is based on questionnaire data conducted on around 113 breeders spread across Polman Regency. The data were analyzed using univariate and bivariate descriptive statistics, chi-square, and odds ratio (OR). The brucellosis model at the farm level was analyzed by multivariate logistic regression. The results of this study indicate the prevalence of brucellosis in Polman Regency is 27.1%. Risk factors associated with diagnosis of brucellosis in Polman Regency included the history of abortion (p=0.000; OR=128), handling of aborted animal (p=0.000; OR=94.667), abortion at the end of pregnancy (p=0.000; OR=62.333), knowledge about brucellosis (p=0.000; OR=9.841), handling of residual abortions (p=0.003; OR=8,471), high grazing density (p=0.000; OR=6.861), introduction of new livestock (p=0.006; OR=5.833), dirty cage (p=0.003; OR=3.600), and grazing method (p=0.005; OR=3.167). Models of diagnosis brucellosis in Polman Regency is = -2,48923 + 3,38734 history of abortion + 2,06312 abortions at the end of pregnancy. The model shows that the history of abortion (β=+3.387; OR=29.588) and the incidence of abortion at the end of pregnancy (β=+2.205; OR = 7.871) can increase the seropositive diagnosis of brucellosis at the farm level.

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