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INDONESIA
LenteraBio
Published by Universitas Negeri Surabaya
ISSN : 22523979     EISSN : 26857871     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Vol 1, No 3 (2012)" : 5 Documents clear
PENGARUH KOMBINASI KOMPOS TRICHODERMA DAN MIKORIZA VESIKULAR ARBUSKULAR (MVA) TERHADAP PERTUMBUHAN TANAMAN KEDELAI (GLYCINE MAX (L.) MERILL) PADA MEDIA TANAM TANAH KAPUR MEGA CHARISMA, ACIVRIDA Mega
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 3 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Soybean productivity should be increased because of the increasingly strong demand. One method is the large of plant area. Therefore calcareous soil can be used as an alternative grown medium, but the contrains in the calcareous soil should be overcome. On of alternative effort to overcome the deficiencies of nutrients in the calcareous soil is the addition of Trichoderma compost and vesicular arbuscular mycorrhizal (VAM). The purpose of this study was to determine the influence of the compost on the soybean grown on the calcareous soil. Experimental study was conducted using Randomized Completely Block Design (RCBD) by a factor of Trichoderma compost treatment (0 g, 15 g, 30 g and 45 g) with six replications. Measured parameters were the percentage of mycorrhizal infection, the percentage of active root nodules, plant height, root length, biomass of soybean. Data were analyzed using one-way ANAVA and continued to by the LSD if there were a significant different. The results showed that of compost consist Trichoderma and VAM affected significantly on the percentage of VAM infection, the percentage of active root nodules, plant height, root length, and biomass plants. The most optimal concentration of Trichoderma compost from all five parameters is 45 grams.
PENGARUH PEMBERIAN ASAM LAURAT TERHADAP APOPTOSIS SEL HELA SECARA IN-VITRO PRANATA EFFENDI, DIDIT Pranata
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 3 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Cervical cancer is the most woman cancer in the world. It caused by Human Papilloma Virus (HPV) which can inactivate some genes that have crucial role on cell cycle and apoptosis. Some cancer treatment such as surgery, chemotherapy and radiotherapy are proved ineffectively. We observed potentially of lauric acid as cancer herbal therapy. Lauric acid has anti free radical activity that important for cancer treatment. This study aim is to observe about the effect of laurid acid on HeLa cell apoptosis and expression of caspase 9 by using in-vitro. The apoptosis of HeLa cell is analyzed by caspase 9 protease enzyme absences by using immunocytochemistry with primary antibody anti caspase 9 and secondary antibody anti rabbit biotin. Percent apoptosis is measured by ratio between numbers of caspase 9 expression HeLa cell and total HeLa cell number times by 100%. The number of cell is counted by microscopy observation using difference color of cell on 5 areas. The brown color show the caspase 9 expression HeLa cell and violet color show the live HeLa cell. The result show that 0,0125 µl/ml of lauric acid giving posses highest caspase 9 expression and percent apoptosis on HeLa cell in-vitro.The apoptosis of HeLa cell is increase along with increasing of lauric acid concentration.
PERTUMBUHAN MISELIUM DAN PRODUKSI TUBUH BUAH JAMUR TIRAM PUTIH (PLEUROTUS OSTREATUS) DENGAN MEMANFAATKAN KULIT ARI BIJI KEDELAI SEBAGAI CAMPURAN PADA MEDIA TANAM SUHARNOWO,
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 3 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Epidermal of soya seed is very abundant but has not maximal utilized yet. One alternative that can be done is to utilize waste as additional media on the white oyster mushroom growing media. This research aim is to compare difference between mycelium growth and fruiting body production of Pleurotus ostreatus on different additional component media, and to get optimal percent combination of growing media. This research design is Completely Randomize Design (CRD) with 5 repetitions. The treatment on this research is bran and epidermal of soya seed with percent combination respectively: 80% : 20%, 50% : 50%, 20% : 80% and 100% epidermal of soya seed. This research parameter is mycelium growth and fruiting body production of Pleurotus ostreatus. This parameter is measured once tree day for 4 observation time. The data is analyzed by one way Anova with 5% significant level. The significant data will be continued by DMRT analysis. The result show that there is significantly different of mycelium growth Pleurotus ostreatus which be planted with different additional component on growing media combination. The growth average of mycelium on different percent of media combination between bran and epidermal of soya seed respectively 80% : 20%, 50% : 50%, 20% : 80% is 2,99 cm; 3,10 cm dan 2,86 cm. On the other result show that there is not significantly different of fruiting body prodution Pleurotus ostreatus which be planted with different additional component on growing media combination. This research suggest that epidermal of soya seed has a potential to substitute for bran and also is better compare with it as Pleurotus ostreatus growing media
KEMAMPUAN TANAMAN GENJER (LIMNOCHARIS FLAVA (L.)BUCH.) MENYERAP LOGAM BERAT TIMBAL (PB) LIMBAH CAIR KERTAS PADA BIOMASSA DAN WAKTU PEMAPARAN YANG BERBEDA HARYATI, MAHARANI
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 3 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

The Paper Industry produces minor product in the form of sewage especially liquid sewage in a very large volume which contains of heavy metals Pb.  Furthermore,  this sewage is usually discharged into the water  which causes pollution. An effort is required to minimize the content of Pb metal before being discharged into waters, including the use of Genjer plants as the agent of phytoremediation. This study aims to determine the ability of Genjer plants to absorb the Lead metals (Pb) from liquid paper waste. The research is accomplished by doing an experiment that uses a Randomized Block Design (RGD) with two treatment factors, the biomass plant and length of exposure time. The treatment factor of plant biomass is 0 g, 50 g, 100 g and 150 g. The factor of exposure time is for 7 days, 14 days and 21 days. The parameters which are observed include the levels of Pb in the liquid paper waste, in roots of Genjer plants and leaves of Genjer plants. The analysis of Pb levels uses ICP that is done at the Energy Studies and Engineering Laboratory, Sepuluh November Institute of Technology (ITS), Surabaya. The data are statistically analyzed using 2-way Anava and further test, Duncant Multiple Range Test (DMRT). The result shows that biomass of Genjer plants and exposure time affect to the ability of Genjer plants to absorb Pb metals in liquid paper waste paper. The most optimal decrease of Pb levels occurs in the treatment of biomass Genjer 150 grams with exposure time for 21 days which is equal to 0.4688 mg / l (80.09%). Genjer plants can absorb and accumulate Pb. The highest accumulation of Pb in the root is 1.1546 mg / l (26.1%), and in the leaves is 0.1120 mg / l (21.9%), both occur at 150 g biomass treatment and exposure time of 21 days. Thus the Genjer plant can be used as fitoremediator heavy Lead metal (Pb) in the liquid paper waste.
PEMANFAATAN SAMPAH ORGANIK SEBAGAI BAHAN PUPUK CAIR UNTUK PERTUMBUHAN TANAMAN BAYAM MERAH (ALTERNANTHERA FICOIDES) NURUL LATIFAH, RIRIS Nurul
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 3 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Waste of the market activity that does not have a good management will cause environmental pollution. The waste is still contain high water content and organic ingredients in the form of carbohydrates, protein, and fat. One way to manage the waste market is to process them into liquid compost (organic liquid fertilizer) because it is more quickly absorbed into the ground and quickly utilized directly by plants, and it does not damage the soil and plants. This study aims to produce the organic liquid fertilizer made ??from vegetable market waste and describing levels of nutrients in fertilizers, as well as determine the effect of dose of liquid fertilizer made ??from waste vegetable market on the growth of red spinach plants (Alternanthera ficoides). This study used a randomized block design (RGD) with 4 doses, including 62.5 ml/L/polybag, 93.75 ml/L/polybag, 125 ml/L/polybag  and 156.25 ml/L/polybag, and urea 0.23 g (control) with five replications. Data obtained from phase 1 was the results of elemental analysis of levels of N, P, K and C/N ratio of organic liquid fertilizer. Data obtained from the phase 2 was the height of plant, leaf number, the length of root, as well as red spinach plant biomass which were analyzed with one-way analysis of variants (one-way ANAVA). Organic liquid fertilizer produced in stage 1 had higher levels of nutrient 0.16% N (average), P 0.014%  levels (very low), and 0.25% K (very low). However, the dose of organic liquid fertilizer made ??from different vegetable market waste does not significantly affect plant growth of red spinach (Alternanthera ficoides).

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