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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 2 Documents
 1 
Search results for , issue "Vol 38, No 01 (2006)" : 2 Documents clear
Efek biji mahkota dewa terhadap ekspresi Anis Widyasari; Yudanti Riastiti; Indwiani Astuti; Rina Susilowati; . Harijadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 38, No 01 (2006)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

The effect of mahkota dewa seed (Phaleria macrocarpa (Schaff. Bowl.) on active caspase-3 expression in WiDr Ca colon cell line Background: Mahkota dewa (MD, Phaleria macrocarapa (Schaff) BoerII.) is widely used for cancer therapy, but its mechanism as anticancer has not been known yet. Recently, ethanol extract of MD seed has been shown to have cytotoxic effect on WiDr Ca colon cell line. Objective: The purpose of this study is to know active caspase-3 expression in WiDr Ca colon cell fine after MD treatment. Methods: WiDr Ca colon cell line was incubated with ethanol extract of MD seed in three concentrations for 48 hours. Active caspase-3 expression was shown using immunohistochemistry method. Results: In the cell line treated with ethanol extract of MD seed 11.35 uglml (1/2 IC50), 22.7 ug/mI (IC,) and 45.4 ug/ml (2x1050), immunopositive cells had been found to be 20.4%, 49% and 63%, respectively. The cell line treated with 5-Fluorouracil (5-FU) in IC50 dosage only had 27.4% imunopositive cell. ConclUsion: Ethanol extract of MD seed had cytotoxic effect on WiDr cell line through the increased active caspase 3 expression. The effect was greater than 5-FU. Key words: Mahkota Dewa, WiDr cell line, Caspase 3, apoptosis, 5 FU
Ekspresi EBNA-1 pada diffuse large B cell lymphoma serta Small lymphocytic lymphoma tipe nodal dan ekstranodal . Indrawati; . Irianiwati; . Harijadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 38, No 01 (2006)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

EBNA-1 expression in nodal and extranodal type of diffuse large B cell lymphoma and small lymphocytic lymphoma Background : Diffuse large B cell lymphoma is the most common type of non-Hodgkin lymphoma. Epstein-Barr Virus infection has been suggested to be involved in the pathogenesis of non-Hodgkin lymphoma. Objective : To know the expression of EBNA-1 in diffuse large B cell lymphoma and small lymphocytic lymphoma and its association with nodal and extranodal location of B cell non-Hodgkin lymphoma Methods : Thirty five cases of B cell non-Hodgkin lymphoma from embedding parafin tissue were constituted the basis of the study. Immunohistochemical examination using CD20 and EBNA-1 monoclonal antibody was done. The difference of EBNA-1 expression between diffuse large B cell lymphoma and small lymphocytic lymphoma and the difference of EBNA-1 expression between nodal and extranodal locations of B cell non-Hodgkin lymphoma were analized by chi square test Result and Conclusion : Sixteen cases of B cell non-Hodgkin lymphoma showed positivity of EBNA-1 expression. There was significant difference between the number of cases with positivity of EBNA-1 expression in nodal and extranodal location of B cell non-Hodgkin lymphoma. There was not significant difference between the number of cases with positivity of EBNA-1 expression in diffuse large B cell lymphoma and small lymphocytic lymphoma. The result supported that latency EBV related lymphoma frequently occurs in extranodal location Key words : EBNA-1, diffuse large B cell lymphoma, small lymphocytic lymphoma, nodal, extranodal

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