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Muhammad Syahrir
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INDONESIA
Pharmaciana: Jurnal Kefarmasian
Published by Universitas Ahmad Dahlan
ISSN : 20884559     EISSN : 24770256     DOI : 10.12928
Core Subject : Health,
Medicine & Pharmacology
Pharmaciana is a scientific journal published by the University of Ahmad Dahlan worked closely with Ikatan Apoteker Indonesia (IAI). Pharmaciana published three times a year, namely March, July and November. with ISSN 2088-4559 and e-ISSN 2477-0256. The article published in the Journal Pharmaciana selected by editors and reviewed by the reviewer. Articles published in Pharmaciana must not be published in other journals or have been previously published. Pharmaciana is indexed in google scholar, ACI (Asean Citation Index), Dimension (Crossreff), Garuda, Sinta, Sherpa Romeo, Index Copernicus International, DOAJ, and BASE. Pharmaciana is accredited by DIKTI (DGHE) of Indonesia No. 105/E/KPT/2022 April 07, 2022
Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)
Articles 24 Documents
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Search results for , issue "Vol 4, No 1 (2014): Pharmaciana" : 24 Documents clear
VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KROMATOGRAFI CAIR KINERJA TINGGI Nining Sugihartini; Achmad Fudholi; Suwidjiyo Pramono; Sismindari Sismindari
Pharmaciana Vol 4, No 1 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.727 KB) | DOI: 10.12928/pharmaciana.v4i1.396

Abstract

High Performance Liquid Chromatography (HPLC) was one of analytical methods commonlyto determine the concentration of epigallocatechin gallate (EGCG) on green tea extract. The methodmust be validated in order to fit to its purpose. The aim of this research was to prove that the usedmethod has selectifity, liniearity, precise, accurate and know limit of detection (LOD) and limit ofquantification (LOQ) is acceptable. The selectifity of analytical method was determined bycalculating the resolution value between two peak. Data from 10 ug/ml and 100 ug/ml with 5replicates would give precition and accuration. Precition was known from CV value and accurationwas known from recovery value in each concentration. Liniearity was known from regression linearbetween concentration and wide area of peak. From regresion linear could calculate LOD and LOQ.Research show that method of analyse have selectificity with Rs= 2,27>1,5; liniearity with r= 0,99;precision with CV 8,74% at concentration 200 µg/ml and 3,74% at concentration 500 µg/ml;accuration with recovery 99,76% at concentration 200 µg/ml and 100,52% at concentration 500 µg/mland the value of LOD is 33,28 ug/ml and LOQ is 110,93 ug/ml.
UJI AKTIVITAS ANTIOKSIDAN FRAKSI ETER HASIL HIDROLISIS INFUSA DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) DENGAN METODE DPPH (1,1-DIPHENIL-2-PICRYLHYDRAZYL) Agustina Ardianti; Any Guntarti; Zainab Zainab
Pharmaciana Vol 4, No 1 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.223 KB) | DOI: 10.12928/pharmaciana.v4i1.391

Abstract

Antioxidant is compounds which have abilities to inhibit oxidation rate. Anredera cordifoliafolium consist of flavonoid which have activities as antioxidant. The purpose of this research was toknow antioxidant activity at ether fraction of hydrolysed aquous extract of Binahong folium (Anrederacordifolia). Ether fraction was obtained by hydrolysing the infuse Binahong folium (Anrederacordifolia) with HCl 2N then fractionated with ether and then evaporated. The antioxidant activity wasmeasured by DPPH method using spectrophotometer. The maximum wavelength and operating timequercetin and samples were 515,0 nm (quersetin) and 514,0 nm (ether fraction), 19-30 minute(quersetin) and 19-29 minute (ether fraction). Scavenging activity test done with the DPPH method(1,1- dyphenyl-2-picrylhydrazyl). The decreasing of DPPH radical solution decreased the absorbantintensity. The percentage of radical scavenging activity was performed as EC50 (EffectiveConcentration). The results indicated that both samples showed free radical scavenging activity withEC50 value for quercetin (2,702±0,15) µg/ml, and ether fraction of (249,31±9,26) µg/ml. Statisticalanalysis using Kruskall Wallis method with 95% of confident level followed by Mann Whitney testgiving evidence the results between samples and positive control (quercetin) are significantlydifference.
EKSTRAKSI ANDROGRAFOLID DARI Andrographis paniculata (Burm.f.) Nees MENGGUNAKAN EKSTRAKTOR SOXHLET Ichwan Ridwan Rais
Pharmaciana Vol 4, No 1 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.565 KB) | DOI: 10.12928/pharmaciana.v4i1.402

Abstract

Chemical component extraction from plant herbal influenced by various aspect, such asextraction technique or chemical component character from the plant. Extraction method and solventpolarity exteremly influence chemical component transfer from the cell of plant to solvent solution.Soxhlet is extraction system instrument with frequently same solvent extraction based on liquid-steamcirculation process of the solvent. Solvent polarity to extract chemical component of the plant herbaldepend on chemical component character it self and ability to penetrate cell membrane.Andrographolide, an active chemical component of Andrographis paniculata has been suggested themost active constituent that responsible to activity of Andrographis paniculata in herbal medication.This chemical constituen has solubility character in solvent with middle polarity that indicates solventelection to extract it. This study shows that chemical component extraction of herbal plant influencedby solvent polarity. Thin Layer Chromatography qualitative analyses indicate that absolute polar andAndrographis paniculatanonpolar solvent could not extract andrographolide, different from middle polarity. Andrographolideextraction with similar solvent and correct method offer extract yield with 16,6% andrographolide.
PENGEMBANGAN FORMULASI MASKER EKSTRAK AIR DAUN ALPUKAT (Persea americana Mill) SEBAGAI ANTIBAKTERI Staphylococcus aureus UNTUK PENGOBATAN JERAWAT Nur Ismiyati; Tri Lestari
Pharmaciana Vol 4, No 1 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.488 KB) | DOI: 10.12928/pharmaciana.v4i1.397

Abstract

Skin problems are often on the spotlight, one of them is acne. Inflammation of acne can betriggered by the bacteria Propionibacterium acne, Staphylococcus epidermidis and Staphylococcusaureus. One of the herbs that can be used for acne treatment is the leaves of avocado (Perseaamericana). This study aims to determine the potential antibacterial activity of avocado leafwater extract on the growth of Staphylococcus aureus bacteria that causes acne and mask-makingformulation preparation. Antibacterial activity test was carried out by the diffusion method and theobserved inhibition zone that occured in a series of concentrations of avocado leaf water extract (15%, 17.5% , 35% , 50% , 75%). Antibacterial activity test was also conducted on some avocado leafwater extract mask formula (17.5% , 35% , 50% , 75%) was evaluated the physical properties of themask and irritation test was done. The results showed that the avocado leaf water extract mask formulahad antibacterial activity greater than the extract form. The physical condition evaluation and irritationtest showed that the best mask formulation was concentration of 35%.

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