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Waste Technology
Published by Universitas Diponegoro
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Education,
Computer Science & IT Other
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Articles 3 Documents
 1 
Search results for , issue "Vol 4, No 2 (2016)" : 3 Documents clear
THE USE OF MOLECULAR MARKERS METALOTHIONEIN PROTEIN FOR MONITORING MARINE POLLUTION DUE TO HEAVY METALS MERCURY IN Apogon beauforti IN AMBON BAY Dominggus Rumahlatu; Fredy Leiwakabessy
Waste Technology Vol 4, No 2 (2016)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1998.848 KB) | DOI: 10.14710/4.2.9-14

Abstract

The heavy metal Hg is a global issue because it is toxic to living organisms. Therefore, monitoring levels of Hg in Ambon Bay is important to do. This is a study about monitoring the levels of heavy metals Hg in the waters of Ambon Bay in 2013 and 2014 by using Apogon beauforti as bio-indicators and Metalothionein-1 (MT-1) as a biomarker to determine the accumulation of Hg in relation to the concentration and expression of MT-1 in the tissue of A. beauforti. The samples used were 50 fish of A. beauforti taken at four different stations with purposive sampling procedure. The analysis of Hg content used AAS method, the expression of MT-1 was determined by western blotting method, and MT-1 concentrations were determined by using ELISA method in the laboratory of Brawijaya University. On the average, the results of the analysis of Hg content indicated that the accumulation of heavy metal Hg varied in four different stations. The levels of Hg in the tissue of A. beauforti in 2014 at station 1 increased, while the levels of Hg at stations 2, 3, and 4 decreased. However, the calculation results of MT-1 concentration by ELISA showed an increase in the concentration of MT-1 in 2014 compared to that in 2013. This result was supported with the fact that the protein tape expressed on the test results of western blotting was very thick. It indicated that the longer the organism exposed to heavy metals Hg, the more increased the accumulation of Hg. Thus, the expression and the concentration of MT-1 would also increase.
ASSESSMENT OF THE PHYSICOCHEMICAL SUITABILITY OF OILS AND FRYING FATS RESIDUALS FOR BIODIESEL PRODUCTION Rubia Pina Luchetti Camargo; Aysha Jussara Ivonilde Carrim; Pedro Ivo Brandão e Melo Franco; Nelson Roberto Antoniosi Filho
Waste Technology Vol 4, No 2 (2016)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.205 KB) | DOI: 10.14710/4.2.1-8

Abstract

Frying food is a quick and tasty way of cooking, but generate a large amount of residual frying oils and fat (RFOF). However, these residuals can be used for making soap, animal food, and biodiesel. Companies have been increasingly interested in developping green solutions to waste management that control its generation, collection, recycling, and disposal. With the increase of biodiesel demand, RFOFs are increasingly used to produce biofuel instead of soap. However, unwanted residuals of RFOFs need to be cleaned and their physical-chemical characteristics need to be analyzed before they can be used for biodiesel production. RFOF was submitted to a Liquid-Liquid Extraction (LLE) to remove highly oxidized compounds and short-chain free fatty acids. Here, we evaluated the physicochemical characteristics and filtered RFOFs to remove waste to improve biodiesel production. We found that that the liquid-liquid extraction (LLE) and filtration using adsorbent decrease first-order oxidized compounds, like hydroxy-dienone, and decrease water content. Those are key parameters to assess quality of raw material for biodiesel production.
Impact Of Discharge Fertilizer Effluents On The Toxicological Profile Of Fish Harvested From A Receiving Creek In Okirika, Rivers State. Kalu Igwe; Kelechi Uche; Uchechi Obasi
Waste Technology Vol 4, No 2 (2016)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.274 KB) | DOI: 10.14710/4.2.15-17

Abstract

The impact of fertilizer effluent discharge on toxicological profile of fish was studied by harvesting fish from Okrika creek which receives treated fertilizer effluent and analysing same for heavy metal (Lead Pb, Nickel Ni, Zinc Z and Copper Cu); Polycyclic Aromatic Hydrocarbon (PAH) and Total Petroleum Hydrocarbon (TPH) concentrations. Similar fish was harvested from Otamiri River which is far from any industrial activity and used as control. Heavy metals were analysed using Atomic Absorption Spectrophotometer while the PAH and TPH were analysed using Gas Chromatography. Result indicated unacceptable high values in most of the studied parameters with significantly higher (P<0.05) values; Pb (8.10±0.02 mg/kg), Ni (1.24±0.12 mg/kg), Zn (22.82±0.13 mg/kg), Cu (2.79±0.16 mg/kg), TPH (1.24±0.01 mg/kg). PAH indicated no significant difference (P>0.05) with value 0.003±0.01 mg/kg. Result indicates an unacceptable high level of these metals in the fish tissue   which is of great concern to consumers and could reflect the level of pollutant in the effluent. The company is thus advised to treat their effluents properly and government ensures compliance.

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