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INDONESIA
Indonesian Journal of Agricultural Science
Published by Kementerian Pertanian
ISSN : 1411982X     EISSN : 23548509     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The journal publishes primary research articles from any source if they make a significant original contribution to the experimental or theoretical understanding of some aspect of agricultural science in Indonesia. The definition of agricultural science is kept as wide as possible to allow the broadest coverage in the journal. Accredited base on Director Decree of Indonesian Institute of Science No. 816/D/2009 with acreditation No. 172/AU1/P2MBI/08/2009. IJAS is newly accredited by the Indonesian Institute of Science in 2012 (No. 473/AU2/P2MI-LIPI/08/2012) for period of 2012-2015.
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Articles 5 Documents
 1 
Search results for , issue "Vol 19, No 2 (2018): December 2018" : 5 Documents clear
SOMACLONAL PUTATIVE MUTANTS OF RICE TOLERANT TO SALINITY Rossa Yunita; Nurul Khumaida; Didy Sopandie; Ika Mariska
Indonesian Journal of Agricultural Science Vol 19, No 2 (2018): December 2018
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v19n2.2018.p67-74

Abstract

Soil salinity could significantly reduce rice yield, therefore, varieties tolerant to salinity are urgent to be developed. Mutation induction could be used to create rice mutants tolerant to salinity. The study aimed to evaluate the tolerance of somaclonal mutants of rice to NaCl salinity in a greenhouse condition and characterize their tolerance mechanism. A total of 45 putative mutants were generated by a gamma ray mutation induction followed with in vitro selection in the growth media containing different NaCl concentrations in the greenhouse experiment. The study consisted of two-factor treatments, namely three levels of NaCl concentrations and 45 rice mutants suspected to be tolerant to salinity, arranged in a completely randomized design. Proline, cations (K, Na, Ca, and Mg) content, and stomata density were evaluated.  The results showed that eight mutants were tolerant to 150 mM NaCl, namely CH30, CH-4-2, II-13-42, II-13-7, II-13-10, II-13-13, II-13- 2, and IA-3-21. These tolerant mutants had a higher Na content compared to the check parent. The tolerant mutants had a high proline content, lower Na, and stable K, Mg and Ca cations as well as had a greater number of stomata and higher stomata length-width ratio. Some of the identified tolerant mutants demonstrated the tolerant mechanism against salinity stress. Further studies are required to evaluate these tolerant mutants in the field conditions under salinity stress.
DIGITAL IMAGE ANALYSIS USING FLATBED SCANNING SYSTEM FOR PURITY TESTING OF RICE SEED AND CONFIRMATION BY GROW OUT TEST Mira Landep Widiastuti; Aris Hairmansis; Endah Retno Palupi; Satriyas Ilyas
Indonesian Journal of Agricultural Science Vol 19, No 2 (2018): December 2018
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v19n2.2018.p49-56

Abstract

The common method used for purity testing of rice seed is human visual observation. This method, however, has a high degree of subjectivity when dealing with different rice varieties which have similar morphology. Digital image analysis with flatbed scanning for purity testing of rice seed was proposed by investigating the morphology of rice seeds and confirmation by grow out test (GOT) in the field. Two extra-long seed varieties were used in this study including a red rice Aek Sibundong and an aromatic rice Sintanur. The identification on 14 parameters of morphological characteristics indicated that only six parameters were correlated, i.e. area, feret, minimum feret, aspect ratio, round, and solidity. The purity of rice seed can be effectively determined using digital image analysis of spikelet color and shape. Based on the discriminant analysis of the digital image the recognition rate of rice seed purity was higher than 99.2% for shape and 93.55% for color. The method, therefore, has a potential to be used as a complement in rice seed purity testing to increase the accuracy of human visual method and it is more sensitive than GOT.
ANALYSIS OF GENETIC DIVERSITY IN TWELVE CULTIVARS OF PEA BASED ON MORPHOLOGICAL AND SIMPLE SEQUENCE REPEAT MARKERS Brijesh Kumar Singh; Monoj Sutradhar; Amit Kumar Singh; Ajay Kumar Singh; Rajendra Prakash Vyas
Indonesian Journal of Agricultural Science Vol 19, No 2 (2018): December 2018
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v19n2.2018.p57-66

Abstract

Pea(Pisum sativum L.)is the second most important legume crop worldwide after chickpea (Cicer arietinum L.) and valuable resources for their genetic improvement. This study aimed to analyze genetic diversity of pea cultivars through morphological and molecular markers. The present investigation was carried out with 12 pea cultivars using 28 simple sequence repeat markers. A total of 60 polymorphic bands with an average of 2.31 bands per primer were obtained. The polymorphic information content, diversity index and resolving power were ranged from 0.50 to 0.33, 0.61 to 0.86 and 0.44 to 1.0 with an average of 0.46, 0.73 and 0.76, respectively. The 12 pea cultivars were grouped into 3 clusters obtained from cluster analysis with a Jaccardd’s similarity coefficient range of 0.47-0.78, indicating the sufficient genetic divergence among these cultivars of pea. The principal component analysis showed that first three principal components explained 86.97% of the total variation, suggesting the contribution of quantitative traits in genetic variability. The contribution of 32.59% for number of seeds per plant, stem circumference, number of pods per plant and number of seeds per pod in the PC1 leads to the conclusion that these traits contribute more to the total variation observed in the 12 pea cultivars and would make a good parental stock material. Overall, this SSR analysis complements morphological characters of initial selection of these pea germplasms for future breeding program.
ANTIMICROBIAL PROPERTIES OF NANO-EMULSION FORMULATED FROM GARLIC, GINGER AND CINNAMON EXTRACTS AGAINST Escherichia coli AND Salmonella typhi Hernani Hernani; Kun Tanti Dewandari
Indonesian Journal of Agricultural Science Vol 19, No 2 (2018): December 2018
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v19n2.2018.p75-82

Abstract

Garlic, ginger and cinnamon had been reported for their antimicrobial activities, for instance against Escherichia coli and Salmonella typhi. The present study aimed to test antimicrobial activities of nanoemulsion of the mixture of garlic, ginger and cinnamon. The nanoemulsion was formulated from the mixture of garlic and ginger extracts and cinnamon essential oil at the ratio of 80:10:10 using a high pressure homogenizer at 300 bars for 5 cycles. The  nanoemulsion powder was prepared using a spray dryer with the inlet and outlet temperatures of 160–170 °C and 70–80 °C, respectively, and maltodextrin as a filler. The nanoemulsion was tested against E. coli and S. typhi. The particle size of nanoemulsion and powdered formulas were characterized using a scanning electron microscope. The LC50 values were identified based on the Brine Shrimp Lethality Test. The results showed that quality of the raw materials confirmed the WHO requirements. The particle size of the emulsion ranged from 151 to 306 nm with the polydispersity index of 0.39–0.52. The 10% and 15% active compounds of the nanoemulsion inhibited E. coli and S. thypi with the LC50 values of 680.15–970.50 ppm and 607.17–903.31 ppm, respectively. The study suggests that the nanoemulsion of a mixture of garlic, ginger and cinnamon extracts could be developed as a food preservative.
HYBRID AND INBRED RICE VARIETIES HAVING HIGH RATOONING ABILITY IN TIDAL SWAMPLAND Susilawati Susilawati; Bambang Sapta Purwoko
Indonesian Journal of Agricultural Science Vol 19, No 2 (2018): December 2018
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v19n2.2018.p83-89

Abstract

Tidal swampland in Indonesia is potential for cultivation of inbred and hybrid rice. However, rice cultivation in this land can be done only one time annually. Rice cultivation with ratoon system in this land might increase production from both the main crop and their ratoon. The study aimed to evaluate the ability of hybrid and inbred rice to produce ratoon and their grain yield in the tidal swampland of Central Kalimantan. The experiment was arranged in a randomized block design with 12 rice varieties as treatments, namely six hybrids (Hipa 3, Hipa 4, Hipa 5, Hipa 6, Maro and Rokan) and six inbreds (Batanghari, Ciherang, IR42, Margasari, Mekongga and Sintanur); replicated three times. Cultivation of the main crop followed the recommendation. At harvest, the main crops were cut at 20 cm from the ground and fertilized with 100 kg ha-1 urea. The results showed that ratoon crops were emerged at 5–6 days after cutting, with 2–4 leaves per panicle and 5.5–26.0 tillers per clump. Average harvesting age of the ratoon was 69 days. Ratoon yield of the hybrids was better than that of the inbreds; the average was 75.2% of the main crops. Based on the criteria of rice yield, three clusters were identified, i.e. high (>2 t ha-1): Hipa 3, Hipa 4, Hipa 5, Maro, Rokan, Ciherang and Sintanur; medium (1–2 t ha-1): Hipa 6, IR42, Margasari and Mekongga, and low, Batanghari. The study suggests that hybrid varieties could be developed in tidal swamplands.

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