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Nexus Kedokteran Translasional
Published by Universitas Sebelas Maret
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Arjuna Subject : -
Articles 6 Documents
 1 
Search results for , issue " Vol 3, No 1 (2014): Nexus Kedokteran Translasional" : 6 Documents clear
The Effect of Mangosteen Peel (Garcinia mangostana L) Extract Against Mice (Mus musculus) Kidney Cell Damage Induced by Paracetamol Qisthina, Namira; Widjokongko, Bambang; Setyawan, Sigit
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Mangosteen peel (Garcinia mangostana L) contains antioxidant such as xanthone’ core, α-mangostin, β-mangostin, γ- mangostin, and garcinone which is thought to be nefroprotector. This study is aimed to investigate the mangosteen peel (Garcinia mangostana L) extract’s nefroprotector effect against mice (Mus musculus) kidney cell damage induced by Paracetamol. Methods: This is an experimental laboratory study with post test only controlled group design. Swiss Webster mice weighing around 20 g were selected as sample. Those mice were divided into 4 groups randomly, each group consist of 7 mice. Incidential sampling was used as a sampling technique. Mice in control group (KK) and first treatment group (KP1) were given aquades, while second treatment group (KP2) and third treatment group (KP3) were given 10 mg  and 15 mg of mangosteen peel extract respectively. KP1, KP2, and KP3 were given 0.1 ml paracetamol on the 8th, 9th, and 10th day of the treatment. Mice were sacrificed on 4th day and the kidneys were made into preparate using paraffin block and HE staining method. Histological kidney cells were assesed by the sum of pyknosis, karyorrhexis, and karyolysis cells. Data were analyzed using One Way ANOVA test continued by Post Hoc Multiple Comparisons (LSD). Result: One-Way ANOVA’s test result showed a significant differences between four groups. LSD’s test result showed a significant differences between KK-KP1, KK-KP2, KK-KP3,KP1- KP2, KP1- KP3, KP2-KP3. Conclusion: Mangosteen peel extract can prevent mice’s kidney cell damage induced by parasetamol. Keywords: Mangosteen peel extract, parasetamol, mice’s kidney cell damage 
The Effect of Mangosteen Rind Extract (Garcinia mangostana L) to the Damaging of Hepar Cell of Mice (Mus musculus) Induced by Paracetamol Panjaitan, Winda A; Widjokongko, S B; Setyawan, Sigit
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: The antioxidant properties of mangosteen rind extract such as xanthones, vitamin B1, vitamin B2, and vitamin C are estimated can protect hepar from free radicals and reducing NAPQI which produced by paracetamol metabolism. The purpose of this research is to know the effect of mangosteen rind ethanol extract to the damaging of hepar cell of mice induced by paracetamol. Methods: This research was a laboratorial experiment with post test only control group design. Samples of 28 male mice, Swiss webster type, 2-3 months old age and ±20 gram of each weight, were divided into 4 groups in random or 7 mice each group. Aquadest were administered to mice in Control Group (KK) and the First Treatment Group (KP1), while mangosteen rind extract were administered to the Second Treatment Group (KP2) and the Third Treatment Group (KP3) with the dose of 10 mg/20 g body weight of mice and 15 mg/20 g body weight of mice. Paracetamol were administered to KP1, KP2, KP3, with dose 5.07 mg/20 g body weight of mice. On day 4th, mice were sacrificed with neck dislocation. Hepar cell slides were made and stained by HE. The hepar cell damage of mice were observed by counting number of necrosis cell. The data was analized by One-Way ANOVA test (α = 0.05) and continued by Post Hoc Multiple Comparisons using Least Significant Differences (LSD) test (α = 0.05). Results: The data showed that average number of necrotic nucleus of KK was 9.71; KP1 was 72.86; KP2 was 14.00; and KP3 was 22.29. The results of One-Way ANOVA test showed a significant difference in all group (p < 0.05). The statistical data result showed a significant difference of necrotic nucleus between K-KP1, K-KP2, K-KP3, KP1-KP2, KP1-KP3, and KP2-KP3 (p < 0.05). Conclusion: The feeding of mangosteen rind was able to decrease hepar cell damage of mice which is induced by paracetamol. Keywords: mangosteen rind extract, paracetamol, the damaging of hepar cell 
Renoprotective Effect of Orthosiphon stamineus Benth. Leaves Extract Against Renal Histological Damage on Mice (Mus musculus) Induced by Reheated Palm Oil Setiawan, Candra Aji; Widjokongko, Bambang; Utari, Siti
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Backgrounds: Orthosiphon stamineus Benth. contains active substances polyphenols which are predominantly in the leaves. Polyphenols are capable to reduce oxidative stress dan halt lipid peroxidation on renal cells because of its antioxidant properties. Palm oil is widely used in Indonesia. It is known that reheated palm oil can damage renal cells by inducing lipid peroxidation reaction. The objective of this study is to know renoprotective effect of Orthosiphon stamineus Benth. to inhibit damage to the renal cells of mice (Mus musculus) induced by reheated palm oil. Methods: This study was an experimental laboratory with a post test only controlled group design. Samples were male Swiss-Webster mice, 2-3 months old and ± 25 g in weight, taken as many as 25 heads by incidental sampling and divided into 5 groups randomly. For 14 days, the Negative Control Group (KK (-)) were given aquadest, the Positive Control Group (KK (+)) were given reheated palm oil, and Treatment Group 1-3 (KP1-3) ​​were given reheated palm oil and Orthosiphon stamineus Benth. leaves extract with multilevel dose 3.5 mg, 7 mg, and 14 mg. On day 15, mice were terminated and the kidneys were made ​​preparations by the method of Paraffin Blocks and HE staining. Kidney histological features assessed by the sum of proximal tubular cells that undergo pyknosis, karyorhexsis, and karyolysis. Data were analyzed using the Kruskall-Wallis test (α = 0.05) and post hoc test of Mann-Whitney (α = 0,05). Results: Kruskal-Wallis test results showed a significant difference among the five study groups p = 0.000 (p < 0,05). Mann-Whitney test results showed significant differences between KK (-) - KK (+), KK (-) - KP1, KK (-) - KP2, KK (-) - KP3, KK (+) - KP1, KK (+ ) - KP2, KK (+) - KP3, KP1 - KP2, KP2 - KP3 (p < 0,05) and a non-significant difference between KP1 - KP3 (p < 0,05). Conclusions: Leaves extract of Orthosiphon stamineus Benth. have renoprotective effects on renal histological damage of mice (Mus musculus) induced by reheated palm oil. Keywords: Orthosiphon stamineus Benth. leaves extract, reheated palm oil, renal histological damage. 
The Effect of Mango (Mangifera indica L.) Juice on Paracetamol – Induced Liver Cell Damage in Mice (Mus musculus) Ras, Samiaji Abbas; Listyaningsih, Endang; Sari, Yulia
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Mango (Mangifera indica L.) contains beta-carotene and vitamin C which were thought to protect the liver from free radicals. In present study, Mango (Mangifera indica L.) juice with certain dose was evaluated for its protective effect on paracetamol induced liver damage in mice. Methods: This study used 28 male Swiss Webster mice were equally divided into four groups. Animals of Control Group (KK) were given aquadest and Treatment Group I (KP1) received paracetamol for 3 days. Treatment Group II and III (KP2 and KP3) not only received paracetamol, but also received multilevel doses of Mango (Mangifera indica L.) juice (0,2 mg and 0,4 mg) for 14 days. Mice were sacrificed on the 15th day and histological preparation was made to evaluate histological damage on liver. Liver histological features  were  assessed  by  counting  the  number  of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. Data were analyzed using the  One Way ANOVA test (α = 0.05) and the Post Hoc Multiple Comparisons (LSD) test (α = 0.05). Results: The mean liver histological damage score was significantly higher on group of KP1= 61,8±7.991, while group of  KK= 26,21±4.828 as the least. KP3 group had fewer numbers of liver histological damage (40,09±7.635) compared to the KP2 group (37,81±5.653). The results of One Way ANOVA test showed significant difference between the five groups (p = 0.000) and the results of LSD test showed significant differences between each other group (p < 0.005). Conclusion: Mango (Mangifera indica L.) juice showed protective effect against the hepatotoxicity induced by paracetamol in mice. Mango (Mangifera indica L.) juice with the dose of 0,4 mg/20 g body weight of mice showed a higher hepatoprotective effect than the dose of 0,2 mg/20 g body weight of mice. Keywords: Mango juice, paracetamol, histologic liver damage.  
The Effect of Acetone Papaya Leaves Extract (Carica papaya, L.) to Hepatoprotector of Rat (Rattus novergicus) Induced by Paracetamol Saputri, Dewantari; ., Suyatmi; Damayanti, Kusmadewi Eka
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Acetone papaya leaves extract contains chlorophyll and flavonoids with antioxidative capacity to protect the liver cell  from free radicals The aims of this research were to know the hepatoprotective effect of the extract and to investigate the influence of increasing dose of the extract towards the amount of  paracetamol-induced liver cells damage of rats. Methods: This study was a laboratory experimental research applying posttest only controlled group design. A total of 24 Strain Wistar rats were equally divided into 6 groups. Rats for control group (KK) and 1st group (KP1) were given aquades  for 7 days. The second treatment group (KP2) was given 100mg/kg body weight (bw) of rat dose curcuma tablet. The 3rd (KP3),  4th(KP4), and  5th (KP5) treatment groups ware given acetone papaya leaves extract dose 150mg/kg bw, 300mg/kg bw, 600mg/kg bw, respectively for 7 days. Paracetamol  was given to KP1, KP2, KP3, KP4, KP5 with dose 600mg/kg weight of rat on 5th, 6th, and 7th day. Finally on  8th day, rat were sacrificed by neck dislocation. After that, we made histological slides from the liver that stained with Hematoxillin Eosin (HE). The slides were observed and assessed based on the liver histological damage (pyknosis, karyorrhexis and karyolysis). Data were analized by Kruskal Wallis (α = 0,05), and continued by Mann Whithney test(α = 0,05). Results: Kruskal Wallis showed that there was a significant difference between 6 groups. Result of Mann Whithney method showed that there was a significant difference between control group  (KK)and treatment group (KP1, KP2,KP4,KP5)  as well as between curcuma (KP2) and acetone papaya leaves extract (KP3,KP4, KP5) groups, but there was no any significant difference between groups the increase doses (KP3-KP4,KP5). Conclusion: Acetone papaya leaves extract has hepatoprotective effect to the liver cell histological damage of rats induced by paracetamol.. Key words : acetone papaya leaves extract, paracetamol, liver cell histological damage.
The Effect of Soybean Powder (Glycine max) to the Histological Structure of Mice’s (Mus musculus) Liver Cells Induced by Used Cooking Oil Anggraeni, Selvia; Suparyanti, Endang Listyaningsih; Febrinasari, Ratih Puspita
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Soybean contain antioxidants which have hepatoprotective effect; one of them is isoflavone. Soybean powder has the highest isoflavone among soybean products. This research aims to prove that the use of soybean powder can prevent damage of the histological structure of the mice’s liver cells induced by used cooking oil. Methods: This study was an experimental laboratory with a post-test only control group design. Twenty eight Swiss Webster male mice were divided into 4 groups. Negative control group (KN) was given distilled water, the positive control (KP) was given 0.06 ml/ 20 g BW used cooking oil, dose 1 (KD1) was given soybean powder 5.6 mg/ 20 g BW and used cooking oil, and dose 2 (KD2) was given soybean powder 11.2 mg/ 20 g BW and used cooking oil. Soybean powder was given for 14 days, while the used cooking oil was given orally on 8th-14th day. On the 15th day, mice were sacrificed, taken their liver, and made ​​preparations with HE staining. Liver cell damage was observed by counting the number of cells with pyknosis, karyorheksis, and karyolysis of 100 cells in liver centrolobular zone. Data were analyzed by statistical tests One-way ANOVA (Analysis of Variant) (α = 0.05). Results: The highest number of liver cells damage is on KP, followed by KD1, KD2, and at least on KN. Results of One-way ANOVA test showed significant differences among the four groups with p = 0.000 (p < 0.05). Results of Post Hoc Test of Multiple Comparasions using LSD test also showed significant differences between KN-KP, KN-KD1, KD2-KN, KP-KD1, KD2-KP, KD1-KD2 with respectively p = 0.000 (p < 0, 05). Conclusion: Soybean powder can prevent damage of the histological structure of the mice’s liver cells induced by used cooking oil. Keywords: soybean powder, used cooking oil, liver histological structure 

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