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BALI MEDICAL JOURNAL (BMJ)
Published by Universitas Udayana
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Bali Medical Journal (BMJ), P-ISSN 2089-1180, E-ISSN 2302-2914 is an international and peer-reviewed journal published quarterly in print and online by Sanglah General Hospital in collaboration to Indonesian Physician Forum and Indonesian College of Surgeon, Bali-Indonesia which was founded in 2011. The Journal aims to bridge and integrate the intellectual, methodological, and substantive diversity of medical scholarship, and to encourage a vigorous dialogue between medical scholars and practitioners. The Journal welcomes contributions which promote the exchange of ideas and rational discourse between practicing educators and medical researchers all over the world.
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Articles 10 Documents
 1 
Search results for , issue "Vol 3 No 3 (2014)" : 10 Documents clear
HEMATOPOIETIC PROGENITOR CELLS AS A PREDICTIVE OF CD34+ ENUMERATION PRIOR TO PERIPHERAL BLOOD STEM CELLS HARVESTING Zulkafli, Zefarina; Mustaffa, Rapiaah; Yusoff, Shafini Mohammed
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
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Abstract

Background: To date, the CD34+ cell enumeration has relied predominantly on flow cytometry technique. However, flow cytometry is time consuming and operator dependent. The application of the hematopoietic progenitor cells (HPCs) channel in Sysmex XE-2100, a fully automated hematology analyzer offers an alternative approach, which is with minimal sample manipulation and less operator dependent. This study evaluates the utility of HPC counts as a predictive of CD34+ enumeration prior to peripheral blood stem cells harvesting. Materials and methods: HPC, CD34+, white blood cell (WBC), reticulocytes (retic), immature platelet fraction (IPF) and immature reticulocyte fraction (IRF) were determined in 61 samples from 19 patients with hematological malignancies (15 lymphoma and 4 multiple myeloma patients) at Hospital Universiti Sains Malaysia (Hospital USM) who had received granulocyte-colony stimulating factor (G-CSF) and planned for autologous transplantation. Results: CD34+ count showed strong and significant correlation with HPC. The receiver operating characteristics (ROC) curve analysis revealed that HPC count > 21.5 x 106 / L can predicts a pre harvest CD34+ count of >20 x 106 / L with sensitivity of 77%, specificity of 64% and area under the curve (AUC) of 0.802. Conclusion: We concluded that HPC count can be a useful potential parameter in optimizing timing for CD34+ enumeration prior to leukapheresis.
COMMUNITY EMPOWERMENT THROUGH ERGONOMICS TRAINING WITH LOCAL WISDOM ORIENTED TO IMPROVE QUALITY OF SCULPTOR HEALTH IN THE PELIATAN VILLAGE, UBUD, GIANYAR, BALI-INDONESIA Sutajaya, I Made
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
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Abstract

Background: Until recently, data applied for reference of Balinese workers in their work stations and processes, including anthropometric data still applying secondary data source from literature review. This was probably inappropriate due to different size, since the literature data generally on the basis of western size of anthropometry. The objective of this research, therefore, was to establish community empowerment through ergonomics training with local wisdom oriented to improve the sculptor health care quality and productivity. Ergonomics training through workshops conducted with a systemic, holistic, interdisciplinary, and participatory (SHIP) approach. The training is done to make people aware of the work as a sculptor that is very important to apply ergonomics in the workplace. The results showed that ( a) participants judge that ergonomics workshop can open their insights about the importance of the application of ergonomics in the workplace, (b) work equipment is not in accordance with sculptor anthropometric, (c) inadequate working conditions, because the workers were exposed to noise working tool more than 80 decibel and the room temperature exceeds 34OC and work a lot of cockroaches and rats roam, and (d) the data of sculptor health quality is very poor, increase the workload at about13.5 %, musculoskeletal complaints at about 41.3 % , and fatigue at about 46.8 % ( p < 0.05 ) between before and after working. That means the work is very necessary sculptor redesigned in order to achieve comfort, safe, healthy, effective, and efficient of working conditions. It can be concluded that the ergonomics training with local wisdom oriented is required in an effort to implement the principles of ergonomics to achieve adequate health care quality sculptors and maximum productivity.
ESTROGEN RECEPTORS OF HAIRS BLACKS AND WHITES Laswati, Hening; Pangkahila, Jahja Alex
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: Aging is termed as same as degenerative process, in which all part of tissue organs retarted the microstructure either macrostructure, forming and function even the colour, including black hair change to white hair. Several researchers have been recommended that estrogen hormone be able ease black to white hair, but hormone without any presenting of receptor won’t be work properly. The main aim of this study were to determine amount of estrogen receptor contents in famales and males black and white hairs included the microscopically structure. Method: Twelve females and males there were 50 -56 years old each pairs black and white head hairs were plucked along with follicles. This estrogen receptors analyzed using radioreceptor binding assay there were 5mm eah hair follices including the root cutted and each pair put its in 2 ml glass tube already filled in with 500 µl 125I-estradiol and incubated in 37oC for 3 hrs. Following times were over the tube flushed twice carefully the hair won’t be flushed. Then count by putting in the gamma counter chamber for 1 minute each. The values that shown in the monitor as CPM (count per minute), recorded as receptor of estradiol. Results: Mean (±SD) sum estrogen receptor in females black and white hairs were 479.3 ± 37.5 and 387.7 ± 33.0, but significantly decreased in male black hair was 316.9±17.8 and 274.0 ± 19.8. All those pairs significantly different either female black and white hairs or male black and white hair and also significantly different among groups. Conclusion: The lowest estrogen receptors recorded in male white hairs and microstructure decreasing of melanin contents.
PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF TWO COMMON TERRESTERIAL MEDICINAL PLANTS RUTA CHALEPENSIS AND RUMEX NERVOSUS Kasimala, Madhu Babu; Tukue, Merih; Ermias, Robiel
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
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Abstract

Background: Ruta chalapensis and Rumex nervosus are used as an extensive household remedy for various diseases in Eritrea. The components of these plants are of great interest in medicinal chemistry. Leaves and young stems of Ruta and Rumex have been reported to contain alkaloids, flavonoids, phenols, amino acids, furanocoumarins and saponins. Various solvents like ethanol, acetone and aqueous extracts of the two plants were screened for the presence of bioactive compounds. Methods: The antibacterial activities of these extracts were investigated against Staphylococcus aureus, a gram positive bacteria and Escherichia coli, gram negative bacteria. The antibacterial activity was tested using Muller Hinton Agar medium by disc diffusion method and minimum inhibitory concentration assays. Results: After incubation, zone of inhibition was measured in mm, a good inhibition (>5mm) was observed indicating the effective antibacterial activity of the bioactive compounds in both the plant extracts.
ADULT MIDGUT MALROTATION WITH LADD’S BAND: A RARE CASE REPORT WITH REVIEW OF LITERATURES Hota, Prasan Kumar; Abhishek, D.; Bhaskar, V.
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
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Abstract

Background: Midgut malrotation is an anomaly of intestinal rotation that occurs during fetal development and usually presents in the neonatal period. It is rare for midgut malrotation to present in adulthood. Method: We present a case of 15 year old girl presenting with acute abdomen of 15 days duration. Clinical evaluation and investigations revealed features of acute small gut obstruction with peritonitis. Patient underwent exploratory laparotomy which revealed Ladd?s band with midgut malrotation with gangrenous and perforated duodeno-jejunal segment. Derotation with release of band with appendicectomy and resection of gangrenous segment with end to end anastomosis and anatomical repositioning of the bowel was performed. Results: Complete resolution of symptoms seen in 4 months follow-up. We report this case for the rarity of the case with an unusual presentation of adult midgut malrotation due to Ladd?s band presenting with gangrene of the affected gut with perforation, which was managed successfully.
LIVER DYSFUNCTION IN DIABETIC PATIENTS ADMITTED IN REFERRAL HOSPITAL Takhelmayum, Roshan; Thanpari, C.; Singh, T. Paras
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
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Abstract

Background: Liver function tests (LFTs) are group of tests that help in diagnosis, monitoring therapy, and assessing prognosis of liver disease. To estimate liver function tests in diabetic patients attending OPD of Central Referral Hospital, Sikkim, India. Methods: This study was conducted in Central Referral Hospital, Gangtok, Sikkim, India between October 2007 to April 2008. A total of 150 diabetic patients and 50 controls were taken to assess the liver function tests (LFTs) by measuring Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline phosphatase (ALP) & total bilirubin (TB). Results: Total Bilirubin (TB) was found to be elevated in maximum number of patients, 84 (56%) out of 150 patients and followed by both AST and ALT, 39 (26%) each out of 150 patients. Conclusion: LFTs in type-2 diabetic patients are found to be statistically significant when compared with normal healthy controls.
SPECIFIC GYRB SEQUENCE OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATED FROM SPUTUM OF PULMONARY TUBERCULOSIS PATIENTS IN INDONESIA Mertaniasih, Ni Made; Wiqoyah, Nurul; Kusumaningrum, Deby; Soedarsono, S.; Perwitasari, Agnes Dwi Sis; Artama, Wayan Tunas
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
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Abstract

Background: Indonesia have many different geographic areas which could be various on the variant strains of Mycobacterium tuberculosis. The gyrB gene codes GyrB protein as sub unit compound of Gyrase enzyme that functioning in multiplication of bacteria. Detection of gyrB gene could be a marker of active multiplication of viable bacteria in the specimen from patients; and some of the DNA sequence regions were conserved and specific in the strain of Mycobacterium tuberculosis that would be a marker for identification. This research aims to analyze the sequence of gyrB gene of Mycobacterium tuberculosis clinical isolates from sputum of pulmonary TB patients in Indonesia, and determine the specific region. Method: Mycobacterium tuberculosis clinical isolates have been collected from sputum of the patients with pulmonary TB that live in some area in Indonesia. Isolation and identification of Mycobacterium tuberculosis clinical isolates using standard culture method; sequence analysis using PCR-direct sequencing of the part bases region of gyrB. Results: this study revealed that nucleotide sequence on a fragment 764 bases of gyrB gene Mycobacterium tuberculosis strains among clinical isolates almost identically to a wild type strain Mycobacterium tuberculosis H37Rv and subspecies member of Mycobacterium tuberculosis complex (MTBC), with a little difference of SNPs; there are many difference nucleotide sequence with MOTT and Gram positive or negative bacteria, except Corynebacterium diphtheria identically with MTBC. Conclusion: the gyrB sequence in Mycobacterium tuberculosis strains among these clinical isolates from sputum of pulmonary TB patients in Indonesia have the conserved specific DNA region that almost identically with wild type strain H37Rv and MTBC.
CORRELATION OF CYSTOSTOMY TO LOW URETHROCUTANEOUS FISTULA INCIDENT IN HYPOSPADIA SURGERY Laura, S. F.; Duarsa, G. W. K.; Mahadewa, T. G.
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
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Abstract

Background: Hypospadia is the most common congenital abnormalities to the penis. The most common post-operative complications and hypospadias is uretherocutan fistula. Uretherocutan fistula is arising from an infected wound, hematoma, urinary extravasations, ischemic necrosis of the flap or graft, technical errors, and postoperative care. Although, reconstruction of the urethra without cystostomy results in patients discharged quickly, some sources still recommend the use of cystostomy in hypospadias surgery. This is because of lower complication rate and more comfortable for the children during the postoperative period. The purpose of this study was to determine the incidence of fistula uretherocutan correlation with cystostomy using hypospadia operation. Methods: This study was carried out with cross-sectional design involving 68 patients, comprising 34 people with operating groups and 34 other people of hypospadias surgery without cystostomy. This is a retrospective study by observing medical record of the patients at Sanglah General Hospital from December 2013 until May 2014. All data obtained were assayed with Chi-square and Person Correlation and considered significant at p < 0.05. Results: This Study reports that the value of r = -0.364, p = 0.034, and PR = 0.16. However, there is a correlation in the use of cystostomy to a lower incidence of fistula in operation uretherocutan hypospadia. In cystostomy surgery, fistula incidence was 2.9% and operating without cystostomy was 17.6%. In operation hypospadia using cystostomy is expected to reduce the incidence of fistula.
COMPARATIVE IMMUNE RESPONSES OF CHILDREN AFTER INTRADERMAL AND INTRAMUSCULAR RABIES VACCINATION Subawa, A. A. Ngurah; Sutirta Yasa, I Wayan Putu; Astawa, Nyoman Mantik
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
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Abstract

Background: Rabies is a cause of death to people within 100% of Case Fatality Rate. Approximately 55.000 people died because of rabies each year, the vast majority of these deaths happen in Asia and Africa. This study aims to find out comparative immune responses of intradermal (ID) and intramuscular (IM) vaccination in children. Method: This was an experimental study to determine antibody response of ID and IM rabies vaccines with randomized pre and posttest control group design. ID and IM vaccination were carried out in 4 replication for each group. A number of 16 children were recruited for each group. Enzyme Linked Immunosorbent Assay (ELISA) was applied to determine titers antibody on day 0, 7, 21, and 28 after vaccination. Results: This study found that titer antibody induced by ID vaccination was lower than IM vaccination. However, the different is not statistically significant in both groups  (p > 0.05). Titers antibody on day 7 after vaccination were 3.08 ± 2.09 IU/ml intradermally and 4.22 ± 3.02 IU/ml intramuscularly. On day 21 and 28 after intradermal vaccination, titers antibody were 6.78 ± 3.52 IU/ml and 12.53 ± 5.92 IU/ml, respectively. Intramuscularly, antibody titers were 9.76 ± 4.86 IU/ml on day 21 and 14.98 ± 7.76 IU/ml on day 28. Conclusion: ID vaccination is safe and can be used as an alternative vaccination for rabies in human. In addition, 0, 7, 21 ID vaccination methods can be recommended for use to control rabies cases in Indonesia because that methods induce protective immune response.
THE ADDITION OF CAFFEINE IN EARLE’S BALANCED SALT SOLUTION MEDIA WITH WASHING UP METHOD INCREASE MEMBRANE INTEGRITY AND ACROSOMAL SPERM Satriyasa, Bagus Komang; Mahendra, Agung Nova
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
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Abstract

Background: caffeine, a methylxanthine derivate, appears to inhibit phosphodiesterase, thereby inhibiting the break down of cAMP and increasing its concentration inside cell. This study aims to assess the effect of caffeine addition in Earles’s Balanced Salt Solution (EBSS) on the increase in membrane integrity and acrosome reaction of spermatozoa using swim up method Methods: This study was carried out at the Clinic of Sexology and Andrology, Sanglah Public Hospital at Denpasar Bali-Indonesia. This study was an experimental study using the design of pre and post test paired control group design. The samples were sperm specimens of eighteen infertile couple male or volunteers who were infertile with age ranged between 20-40 years old. The samples were divided into two groups: treatment group (caffeine + EBSS) and control group (EBSS). The data were analysed statistically by normality test (Kolmogorov - Smirnov Goodness of Fit Test), Homogeneity test, and Paired Student’s t test. Results: The results showed that the caffeine addition in EBSS medium could increase significantly (p<0.05). The integrity of the sperm membrane obtained were from 81.30 % to 86.60 % and acrosomal reaction from 82.60% to 89.60% evaluated by hypo-osmotic swelling test (HOS). The conclusion of this study is that addition of caffeine in EBSS medium increases significantly membrane integrity and acrosomal reaction of the human sperm.

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