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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 17 Documents
 1   2 
Search results for , issue " Vol 10, No 2 (2010)" : 17 Documents clear
RESPON TANAMAN PADI GOGO (Oryza sativa L.) TERHADAP STRESS AIR DAN INOKULASI MIKORISA Sukiman, Harmastini; Adiwirman, Adiwirman; Syamsiyah, Syofiatin
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (961.468 KB) | DOI: 10.14203/beritabiologi.v10i2.1979

Abstract

It has been known that upland rice production is lower than low land rice production because it is inhibited by soil fertility, water supply and pest.One solutions to increase the production of upland rice is by applying the potential soil microorganism known as mycorrhizae.The objective of this research is to know thef Vesicular Arbuscular Mycorrhizae (VAM) role for upland rice under water stress condition. The research was conducted in greenhouse of Cikabayan, Bogor Agricultural University and Soil Microbiology Laboratory of Research Centre of Biotechnology, Indonesian Institute of Science Centre. The experimental design was factorial randomized block design with two factors. The first factor is inoculation of mycorrhizae consisted of two treatment i.e. without mycorrhizae (MO) and with mycorrhizae (Ml); while the second factor is water stress regime consisted of five treatments namely well watered (SO), water stress on tillering stage (SI), primordial stage (S2), anthesis stage(S3) and grain filling stage(S4). Mycorrhizae significantly increased root infection, phosphate uptake, height, number of tillering, leaf, Leaf Area Index (LAI), productive tillering, filled grain, grain weight and yield. While, water stress significantly decreased number of root infection, shoot wet fresh weight, root dry weight, increased phosphate uptake and sterilized grain, but it didnt significantly decrease yield. The interaction both mycorrhizae and water stress didnt significantly influence all variables, except the root dry weight. The inoculation of mycorrhizae could increase 19.62% on SI, 17.32% on S2, 29.14% on S3, 6.89% on S4 dried harvested yield.
CATATAN JENIS-JENIS TUMBUHAN ASING DAN INVASIF DI TAMAN NASIONAL GUNUNG GEDE-PANGRANGO, JAWA BARAT Sunaryo, Sunaryo; Tihurua, Eka F
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (554.896 KB) | DOI: 10.14203/beritabiologi.v10i2.1981

Abstract

Menurut International Union for Conservation of Nature and Natural Resources (IUCN )Species Asing Invasif merupakan jenis-jenis tumbuhan asing yang berkembang dan menyebar di luar habitat aslinya, sehingga mengancam ekosistem, habitat, atau jenis yang lain. Species Asing Invasif dapat berasal dari habitat yang berada di luar pulau, kepulauan, benua ataupun negara aslinya. Lebih lanjut The Invasive Sepscies Advisory Committee (ISAC) menegaskan bahwa gangguan terhadap suatu ekosistem akibat masuknya Species Asing Invasif dapat menimbulkan kerugian-kerugian ekonomi, kerusakan lingkungan dan juga gangguan kesehatan manusia.
PENGGUNAAN VAKSIN Aeromonas hydrophila: PENGARUHNYATERHADAP SIANTAN DAN IMUNITAS LARVA IKAN PATIN (Pangasionodon hypophthalmus) Lusiastuti, Angela Mariana; Hadie, Wartono
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (892.062 KB) | DOI: 10.14203/beritabiologi.v10i2.1967

Abstract

Aeromonas hydrophila is a pathogen that often causes considerable losses in the area of freshwater fish fanning. Vaccination is one way to simulate parent catfish make specific immunity. Specific immunity generated by the parent will be forwarded through the oocytes produced during a certain time span. The aim of this research was to know the effect and the effectivity of using hydrovac vaccine with and without the complete adjuvant. This research was done on Patin fish Pangasionodon hypophthalmus whose givng Hydrovac 0.4 ml/kg of body weight. The comparation between complete adjuvant and vaccine was 1:1. Injection was done by intra peritoneal for three mothers each with and without complete adjuvant. Injection was done at gonad maturity level II. The result showed that antibody were positively detected on mother serum which used adjuvant or not. On larva stage, antibody was detected until four weeks old. While on 2 weeks old of larva, the concentration of titer antibody was very high and raised the dilution of 1: 2048. Survival rate of juvenile which their mother got a vaccine raised 93%, was better than 73%-63% using mother without vaccine. Booster immersion of hydrovac vaccine could give preferably at the end of three weeks old or in the beginning of fourth weeks old of larva.
PENGARUH EKSTRAK AIR DAN ETANOL Kaempferia spp. TERHADAPAKTIVITAS DAN KAPASITAS FAGOSITOSIS SELMAKROFAG YANG DIINDUKSI BAKTERI Staphylococcus epidermidis Murningsih, Tri
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1977

Abstract

Ethanol 95% and water extracts of Kaempferia spp. (K. galanga, K. angustifolia, K. pandurata and K. rotunda) were tested for their influences in in-vitro phagocytosis activity and phagocytosis capacity of mouse peritoneum macrophage cells induced by Staphylococcus epidermidis. Extracts were tested at a series concentration in logarithmic order (0.1-1000) ug/ml. Imboost and distilled water were employed as positive and negative controls, respectively. The results shows that almost all extracts were capable to increase on phagocytosis activity (SPA - Screening for Phagocytosis Activity) and phagocytosis capacity (IP • Index Phagocytosis) of macrophage cells compared to positive and negative controls significantly (p<0.05).The phagocytosis activity and capacity macrophage cells were increased by increasing extract concentration. K.rotunda extracts shows reveal better on above activities in low concentration (0.1-100) ug/ml then other species.
KEANEKARAGAMAN LUMUT DITAMAN NASIONAL BUKIT BARISAN SELATAN, PROVINSI LAMPUNG Windadri, Florentina Indah
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1074.549 KB) | DOI: 10.14203/beritabiologi.v10i2.1968

Abstract

A survey on mosses diversity has been carried out in Bukit Barisan Selatan National Park at Lampung Province. Results of this study indicated that there are 37 species of mosses included in 3 genera and 11 families. Five species are considered as new records lo Sumatera i.e. Arthrocormus schimperi, Calymperes mountleyi, Fissidens perpusillus, Ectropotheciella distichophylla and Taxithelium lindbergii. Two species were suggested as new records to Indonesia i.e. Ectropothecium ferrugineum and Mnium laevinerve. While Ectropothecium ferrugineum is found in the research area, its endemic status is changed and the distribution area become more spreaded.
VIRULENCE OF Xanthomonas oryzae pv. oryzae AND REACTION OF RICE GENOTYPES TO THE RACES OFTHE PATHOGEN [Vimlensi Xanthomonas oryzae pv. oryzae dan Reaksi Genotipe Padi Terhadap Ras Patogen] Suryadi, Y; Kadir, Triny S
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1973

Abstract

The objective of the trials is to study host-pathogen relationships under green house test and field experiment.The virulence of Xanthomonas oryzae pv. oryzae (XOO) isolates of races III, and VIII is characterized on rice genotypes with different resistance to bacterial blight (BB) disease. The result shows that the multiplication of the bacterial population on the resistant (IR 36) and susceptible (TN-1) rice genotype is almost similar at initial Aage of infection; however, BB symptom expression as indicated by lesion length and area under disease progress curve (AUDPC) are much faster on TN-1 than that on IR 36 genotype. Based on rice field trial in Pusakanagara Expt. St; W. Java; almost all rice genotypes tested shows susceptible (S) to highly susceptible (HS) against XOO race VIII at generative stage observation.
KERAGAMAN BAKTERI ENDOFITIK DnSOLASI DARI EMPAT VARIETAS PADI DENGAN METODA ARDRA Susilowati, Dwi N; Hidayatun, Nurul; Tasliah, Tasliah; Mulya, K
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1978

Abstract

Sixty eight endophytic bacteria were isolated from four different rice varieties (IR64, Cirata, Code and Limboto) obtained from agroecosystem in Cikembar, Sukabumi, West Java. Those isolates were subjected for analysis the diversity based on genetic fingerprinting through Amplified Ribosomal DNA Restriction Analysis (ARDRA) method. The objective of this research is to characterize the predominant endophyte bacteria are present within various rice varieties grown on agroecosystem in Cikembar, Sukabumi by using ARDRA method. The results shows that restriction analysis with both Rsal and HaelH was sufficient to allocate the endophyte bacteria from four different rice varieties into the 29 types. Moreover, Rsal alone was capable of resolving the 11 types, followed by HaelH 14 types. In general, the result may explain that there is no collinierity between the cluster and their host plant. Staphylococcus, Bacillus, Agrobacterium, Serratia, Klebsiella, Acidovorax and Pseudomonas were identified as endophytic bacteria from rice varieties in this agroecosystem based on their 16S rRNA sequences. Seven types were placed in close proximity to these genera, but other types were still unknown. Among these isolates, genera Staphylococcus and Bacillus are common to rice endophytes.
PRIMER-PRIMER BARU UNTUK MENGAMPLIFIKASI GEN PENGKODE PROTEIN AMPLOP VIRUS DENGUE STRAIN CH53489 Djajanegara, Ira
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (694.396 KB) | DOI: 10.14203/beritabiologi.v10i2.1969

Abstract

Restriction site of BamHI and Sail must be added in order to express the gene encoding envelope protein of dengue virus strain CH53489 (gene E) into expression vector pMAL-p2x. This approach required the PCR technique for amplification as well as restriction sites addition. However, PCR amplification is prone to error due to the process of misincorporation eventhough using Platinum taq polymerase. Therefore, it is important to be concern that there will be no alteration of the gene especially for biopharmaceutical purposes such as recombinant vaccine. This experiment was aimed to design several primers of DenV-M F, D3-1715s, D3-2117s, D3-1911c and DenV-M R for full length sequencing of the amplified products. Primers were designed in silico using Oligo Explorer and tried in vitro to check the ability of the primers to produce fragments. The sequencing results showed that the amplified product suffered from misincorporation during amplification (98.9% homology). However, the 3-D protein structure prediction did not show any major changes in the protein structure. Further analysis of the expressed protein is required to be used for biopharmaceutical purposes.
KEANEKARAGAMAN TUMBUHAN PULAU SEPANJANG JAWA TIMUR Rugayah, Rugayah; Suhardjono, Suhardjono; Susiarti, S
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1069.393 KB) | DOI: 10.14203/beritabiologi.v10i2.1974

Abstract

Sepanjang Island is a small island located in the eastern part of Madura Island in the Province of East Java. An exploration has been carried out in 200S to record plant diversity of this area. More than 2S0 species of plants were recorded including the cultivated ones. Apocynaceae, Euphorbiaceae, Fabaceae, Malvaceae, Meliaceae, Moraceae, Rhamnaceae and Vitaceae were dominant in this island. Aglaia lawii, Anadendron sp., Tetrastigma lanceolarum, Uvaria littoralis, Zyzyphus jujuba were common in all study sites. Eleven collections are new records for Flora of Jawa and 29 numbers are new collection for Herbarium Bogoriense from this island.
SIDIK JARI DNA PLASMA NUTFAH PADI LOKAL MENGGUNAKAN MARKA MOLEKULER SPESIFIK UNTUK SIFAT PADI BERAS MERAH Utami, Dwinita W; Ilhami, Aderahma; Hanarida, Ida
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (946.934 KB) | DOI: 10.14203/beritabiologi.v10i2.1966

Abstract

Red rice is one corp that has genetic variation both based upon phenotype and genotype evaluation using moleculer markers for specific gene. A research objective is to identify genetic diversity using red rice specific molecular markers. Forty accessions of Indonesian local rice included 9 accessions of red rice germplasm were used as genetic materials for this research. Specific molecular markers (RC3, RC9 and RC12) for a pigment color found in rice seed were designed based on alignment sequence analysis to rc-bHLH gene, a transcription factor for prothocyanidine protein. Then these markers were used to analyze the genetic variation of red rice. Cluster analysis and association test between phenotype and genotype performances were analyzed by TASSEL 2.1 software program. Results showed that red rice accessions distributed into 2 cluster. One cluster is more closely related to white rice than the other. The association test showed RC12 is the most significant marker in association with the red rice trait: red pericarp and white aleuron. These phenotype variations were found in Cempo merah, one accession originated from Yogyakarta and Pulut mandoti, one accession from South of Sulawesi.

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