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REGENERASI IN VITRO EMPAT VARIETAS KEDELAI (Glycine max [L.] Merr.) MELALUI ORGANOGENESIS MENGGUNAKAN EKSPLAN BIJI YANG DIIMBIBISI DAN DIKECAMBAHKAN Fauzi, Mohammad Irham; Yelli, Fitri; Edy, Akari; Utomo, Setyo Dwi
Jurnal Agrotek Tropika Vol 2, No 2 (2014)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Penelitian dilakukan untuk mengetahui pengaruh metode pra-kultur yang efektif dalam meningkatkan efisiensi regenerasi in vitro terhadap eksplan buku kotiledon pada empat varietas kedelai. Penelitian dilaksanakan di Laboratorium Ilmu Tanaman Fakultas Pertanian Universitas Lampung, dimulai dari Maret sampai dengan Mei 2013. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak kelompok. Perlakuan yang diterapkan merupakan kombinasi dari dua faktor yaitu varietas (Detam 1, Detam 2, Burangrang, Panderman) dan metode pra-kultur (kecambah 6 hari dan imbibisi 20 jam) yang merupakan perlakuan terhadap empat varietas benih kedelai tersebut sebelum ditanam pada media inisiasi tunas. Perlakuan disusun secara faktorial (4x2) dengan 5 ulangan. Setiap satuan percobaan terdiri dari empat eksplan buku kotiledon kedelai. Hasilpenelitian menunjukkan bahwa rata-rata jumlah tunas adventif per eksplan (RJTAPE) tidak dipengaruhi oleh metode prakultur, varietas dan interaksi antara keduanya. Sedangkan, persentase eksplan yang membentuk tunas adventif (PEMTA) tidak dipengaruhi oleh metode pra-kultur namun dipengaruhi oleh varietas dan interaksi antara kedua faktor tersebut. PEMTA tertinggi didapatkan jika menggunakan varietas Detam 1 melalui perlakuan perkecambahan (70%) dan varietas Pandermanmelalui perlakuan imbibisi (50%). Media pengakaran Â½ MS dan Â½ MS + NAA 0,5 mg l-1 memiliki efektifitas yang sama terhadap persentase tunas adventif yang membentuk akar fungsional (PTMAF) pada minggu kedua setelah pengakaran. Dengan demikian, teknik regenerasi secara in vitro melalui organogenesis pada varietas Detam 1 dengan perlakuan perkecambahan dan varietas Panderman dengan perlakuan imbibisi lebih efisien digunakan untuk transformasi genetik kedelai.

Seleksi Lima Isolat Fungi Mikoriza Arbuskular untuk Pembibitan Kelapa Sawit (Elaeis guineensis Jacq.) pada dua Dosis Pupuk NPK Rias, Retta Ramadhina; Rini, Maria Viva; Yelli, Fitri
Jurnal Penelitian Pertanian Terapan Vol 15, No 1 (2015)
Publisher : Politeknik Negeri Lampung.

Oil palm (Elaeis guineensis Jacq.) in Indonesia commonly cultivated on land that is classified as marginal land such as soil type ultisol with a low fertility rate, so it is necessary to improve the quality of seeds with the use of arbuscular mycorrhiza fungi (AMF) and NPK fertilizers. This study aimed (1) to determine the most appropriate type of AMF for oil palm seedling, (2) to determine the dose of NPK fertilizeris best for the growth of oil palm seedlings, (3) to determine whether the response of oil palm seedling stobe determined by the type of NPK fertilizer, (4) to determine the best dose of NPK fertilizer for each type of AMF. Research compiled using factorial treatment design (6 x2) with 5 replications. The first factor is the type of mycorrhizal (M) which consists of 6 levels iem0 (without AMF), m1 (Entrophospora sp. Isolates MV 3 ), m2 (Entrophospora sp. Isolates MV12), m3 (Glomus sp. Isolates MV 4), m4 (Glomus sp. Isolates MV11), and m5 (Glomus sp. Isolates MV13). The second factoris the dose of NPK fertilizer (P) which consists of 2 level p1 (100% of the recommended dose), p2 (75% of the recommended dose). The similarity of variance between treatments were tested with Least Significant Differencetest (LSD) at 5% significance level . The results showed (1) All types of FMA being tested can increase the growth of oil palm seedlings compared without exception AMF Glomus sp. MV isolates 11, (2) dose of NPK fertilizer 100% of recommended doses produce the best growth of oil palm seedlings shown by the fresh weight of shoot, dry weight of shoot, fresh weight of root, dry weight of root,and leaf greenness level, (3) palm seedling growth response to AMF inoculationis not determined by a given dose of NPK fertilizer, (4) there is no optimum dose of NPK fertilizer for each type of AMF were used. Keywords :Arbuscular mycorrhizal fungi, dose NPK fertilize, oil palm seedling.

PENGGUNAAN AIR KELAPA DAN ASPIRIN UNTUK PRESERVASI UBI JALAR (IPOMEA BATATAS) SECARA IN VITRO Yelli, Fitri
JURNAL AGROTROPIKA Vol 19, No 1 (2020): Jurnal Agrotropika Vol 19 No 1, Mei 2020
Publisher : JURNAL AGROTROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (738.636 KB)

Sweet potato is raw locally food used as an alternative staple food that important for national food security. The objective of the reseach was to achive the suitable medium for preservation of sweet potato plants by slowly growth in in vitro medium. The factorial experiment arranged in completely randomized design with the first factor was two concentrations of MS medium: MS 1 and MS 2. The second factor was several concentrations of coconut water, i.e. 0 and 15 mg/l and the third factor was several concentrations of Aspirin (0, 5, 10, 15, 20 mg/l) . The results showed that the number of green leaves and the number of internodes were affected by concentrations of medium in six weeks after planting. The MS1 medium with 0 mg/l Aspirin and 15 mg/ coconut water could produced the higher number of green leaves (8,0) and 8,5 internodes number. The MS2 + 15 mg/l coconut water and 15 mg/l Aspirin was the best medium for sweet potato preservation. Data obtained also showed that all medium containing Aspirin produced the high number of root. Keywords : Sweet potato, coconut water, Aspirin, preservation, in vitro.

Seleksi Lima Isolat Fungi Mikoriza Arbuskular untuk Pembibitan Kelapa Sawit (Elaeis guineensis Jacq.) pada dua Dosis Pupuk NPK Retta Ramadhina Rias; Maria Viva Rini; Fitri Yelli
Jurnal Penelitian Pertanian Terapan Vol 15 No 1 (2015)
Publisher : Politeknik Negeri Lampung.

Oil palm (Elaeis guineensis Jacq.) in Indonesia commonly cultivated on land that is classified as marginal land such as soil type ultisol with a low fertility rate, so it is necessary to improve the quality of seeds with the use of arbuscular mycorrhiza fungi (AMF) and NPK fertilizers. This study aimed (1) to determine the most appropriate type of AMF for oil palm seedling, (2) to determine the dose of NPK fertilizeris best for the growth of oil palm seedlings, (3) to determine whether the response of oil palm seedling stobe determined by the type of NPK fertilizer, (4) to determine the best dose of NPK fertilizer for each type of AMF. Research compiled using factorial treatment design (6 x2) with 5 replications. The first factor is the type of mycorrhizal (M) which consists of 6 levels iem0 (without AMF), m1 (Entrophospora sp. Isolates MV 3 ), m2 (Entrophospora sp. Isolates MV12), m3 (Glomus sp. Isolates MV 4), m4 (Glomus sp. Isolates MV11), and m5 (Glomus sp. Isolates MV13). The second factoris the dose of NPK fertilizer (P) which consists of 2 level p1 (100% of the recommended dose), p2 (75% of the recommended dose). The similarity of variance between treatments were tested with Least Significant Differencetest (LSD) at 5% significance level . The results showed (1) All types of FMA being tested can increase the growth of oil palm seedlings compared without exception AMF Glomus sp. MV isolates 11, (2) dose of NPK fertilizer 100% of recommended doses produce the best growth of oil palm seedlings shown by the fresh weight of shoot, dry weight of shoot, fresh weight of root, dry weight of root,and leaf greenness level, (3) palm seedling growth response to AMF inoculationis not determined by a given dose of NPK fertilizer, (4) there is no optimum dose of NPK fertilizer for each type of AMF were used. Keywords :Arbuscular mycorrhizal fungi, dose NPK fertilize, oil palm seedling.

Response of Biofertilizer Application and Alkali Supplement Fertilizer on the Growth and Yield of Curly Red Chili (Capsicum annuum L.) Devi Puspita Amartha Yahya; Kus Hendarto; Fitri Yelli; R.A. Diana Widyastuti
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 11, No 1 (2022): March
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v11i1.15-23

This research aimed to determine the effect of biofertilizer application and alkali supplement fertilizer on the growth and yield of chili (Capsicum annuum L.) This research is arranged in a randomized block design (RBD) consisting of two factors and three replications. The first factor is biofertilizer with three levels, i.e., H0=without biofertilizer; H1= dose of 4 ml/plant; H2= dose of 8 ml/plant. The second factor is alkali supplement fertilizer with three levels, i.e., P0=without alkali supplement fertilizers; P1= dose of 0,05 gr/plant; P2= dose of 0,1 gr/plant. The results showed that the growth variable did not have a significant interaction effect between biofertilizer and alkali supplement fertilizer. Meanwhile, in production variables, there are interaction effects between the application of biofertilizers and alkali supplement fertilizer in variables of number of flowers, number of fruit consumption, and fruit weight per plant. The biofertilizer application with a dose of 8 ml/plant accompanied by alkali supplement fertilizers dose of 0,1 gr/plant resulted in the highest fruit weight, 436.70 grams/plant. The biofertilizer application of 8 ml/plant produced 402.12 grams/plant3, while the application of alkali supplement fertilizer with a dose of 0,1 gr/plant produces chili production of 400.51 grams/plant.Keywords: alkali supplement fertilizer, biofertilizer, chili

EFISIENSI REGENERASI IN VITRO MELALUI ORGANOGENESIS EMPAT VARIETAS KEDELAI (Glycine max [L].Merr.) DARI EKSPLAN BIJI YANG DIKECAMBAHKAN ATAU DIIMBIBISIKAN Ria Aprilenta; Fitri Yelli; Setyo Dwi Utomo; Akari Edy
Jurnal Agrotek Tropika Vol 2, No 2 (2014)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Penelitian ini bertujuan mengetahui pengaruh perlakuan eksplan biji yang dikecambahkan atau diimbibisikan (prakultur) terhadap efisiensi regenerasi in vitro empat varietas kedelai (Glycine max (L).Merr.) melalui organogenesis. Penelitian dilakukan di Laboratorium Kultur Jaringan, Fakultas Pertanian, Universitas Lampung, dari bulan Maret 2013 sampai dengan Mei 2013. Percobaan disusun dalam rancangan acak kelompok yang terdiri atas 5 ulangan. Perlakuan disusun secara faktorial (4x2) dengan faktor pertama adalah varietas kedelai sebagai eksplan (Grobogan, Argomulyo, Tanggamus, dan Ijen) dan faktor kedua adalah perlakuan pra-kultur (imbibisi atau pengecambahan). Setiap satuan percobaan terdiri atas empat eksplan yang dikulturkan dalam satu botol. Hasil penelitian menunjukkan bahwa perlakuan pra-kultur berpengaruh terhadap persentase eksplan yang menghasilkan tunas adventif (PEMTA)dan rata-rata jumlah tunas adventif per eksplan (RJTA). Sedangkan perlakuan varietas dan interaksi hanya berpengaruh pada PEMTA tetapi tidak berpengaruh pada RJTA. Pada perlakuan imbibisi, PEMTA varietas Ijen lebih tinggi daripada Grobogan, Argomulyo, dan Tanggamus. Pada perlakuan pengecambahan, PEMTA varietas Ijen lebih tinggi daripada Argomulyo namun tidak berbeda dengan Tanggamus dan Grobogan. Jika menggunakan varietas Ijen dan Argomulyo, PEMTA perlakuan imbibisi lebih tinggi daripada pengecambahan. RJTA perlakuan pra-kultur imbibisi 20 jam (19,5 tunas per eksplan) lebih tinggi daripada perlakuan kecambah 6 hari (9,63 tunas per eksplan). Selain itu, media pengakaran Â½ MS tanpa NAA lebih baik dalam membentuk akar fungsional daripada Â½ MS yang mengandung NAA 0,5 mg/l.

PENGARUH PUPUK KANDANG DAN PUPUK HAYATI PADA PERTUMBUHAN DAN PRODUKSI TANAMAN TOMAT (Lycopersicum esculentum Mill.) Purba Sanjaya; Novi Kurnia; Kushendarto Kushendarto; Fitri Yelli
Jurnal Agrotek Tropika Vol 9, No 1 (2021): JURNAL AGROTEK TROPIKA VOL 9, JANUARI 2021
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v9i1.4895

Produktivitas tanaman tomat di Provinsi Lampung terus menurun dalam beberapa tahun terakhir. Pada Tahun 2017 tercatat produktivitas tomat mencapai 13.46 ton/ha, sedangkan pada tahun 2019 hanya mencapai 10.52 ton/ha. Bahkan nilai ini masih jauh lebih rendah dibandingkan dengan rata rata nasional yang mencapai 18.63 ton/ha. Untuk mengatasi hal tersebut peningkatan produktivitas mutlak dibutuhkan, salah satunya adalah dengan penambahan pupuk kandang dan pupuk hayati. Penelitian ini dilaksanakan di Laboratorium Lapang Terpadu, Fakultas Pertanian, Universitas Lampung pada bulan Januari - Mei 2020. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) secara faktorial 4 x 3 dengan tiga ulangan. Faktor pertama adalah pemupukan dengan menggunakan beberapa jenis pupuk kandang (ayam, kambing dan sapi) sebanyak 1/3 bagian media tanam, sedangkan faktor kedua adalah aplikasi pupuk hayati dengan dosis, 0 l/ha, 4 l/ha, dan 8 l/ha. Hasil penelitian menunjukkan bahwa aplikasi pupuk kandang berpengaruh nyata terhadap seluruh variabel pengamatan. Dari ketiga jenis pupuk kandang, pupuk kandang kambing memberikan hasil yang paling baikdibandingkan pupuk kandang lainnya. Aplikasi pupuk hayati dengan dosis 4 l/ha berpengaruh nyata terhadap variabel jumlah cabang primer dan jumlah bunga tanaman tomat dan merupakan perlakuan terbaik untuk pupuk hayati. Tidak terjadi interaksi antara perlakuan pupuk kandang dan pupuk hayati.

PENGARUH FORMULASI BIOCHAR DAN LIMBAH KULIT KOPI TERHADAP PERTUMBUHAN BIBIT KOPI Fitri Yelli; Hanisah Hanisah; Rusdi Evizal; Sugiatno Sugiatno
JURNAL AGROTROPIKA Vol 19, No 2 (2020): JURNAL AGROTROPIKA VOL.19 NO. 2, Oktober 2020
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v19i2.4544

Coffee husk is the main waste of coffee bean production using a dry processing system. Coffee husk is returned to the coffee plantation as mulch, or used as a mixture for nursery media and for making bio-charcoal. This study aims to determine the effect of the formulation of biochar, coffee husk, and soil on the growth of Arabica coffee seedlings. The experiment used a randomized block design (RBD) consisting of 6 treatments with 4 replications. The treatments were the composition of the ratio of biochar: coffee husk: soil (v / v / v), namely: 0: 1: 2 (control, without biochar), 1: 1: 2, 1: 0: 2 (without coffee skin), 2: 1: 2, 1: 2: 2, and 1: 1: 1. The coffee husk biochar is made by a slow burning system in a stack. The results showed that: (1) The treatment of biochar composition, coffee husk and soil had an effect on the number of leaves, length x leaf width, crown dry weight, fresh weight quality index, and dry weight quality index; (2) biochar mixing treatment did not increase seedling growth. Mixing biochar with a composition of 1: 1: 2 reduces root growth; (3) the composition of biochar, coffee husk and soil 1: 1: 2 gave better seedling growth than the composition 0: 1: 2 (control without biochar) based on the quality index of fresh seedling and dry seedling.Keywords: Arabica, biochar, coffee husk, growth, seedling, quality indeces

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