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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Aisyah : Jurnal Ilmu Kesehatan
Resna Hermawati
Departemen/ Instalasi Patologi Klinik Fakultas Kedokteran Universitas Airlangga/ RSUD Dr Soetomo Surabaya
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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RELATIONSHIP BETWEEN SPECIFIC GRAVITY OF CUPRIC SULFATE AND SATURATION OF BLOOD DROPLETS DURING DONORS’HEMOGLOBIN SCREENING Resna Hermawati; Paulus Budiono Notopuro; Solichul Hadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1376

Abstract

There are several methods of hemoglobin screening. A technique that is practical, easy, and fast,as well as does not require high costs is needed for blood donor activities. Cupric sulfate method is still used in blood donor activities nowadays. There are several types of the specific gravity of cupric sulfate which will determine saturation speed. On the other hand, one of the requirements for a blood donor, according to the WHO, is that its hemoglobin level must be ≥ 12.5 g/dL. This research aims was to reveal how many blood droplets could be dripped into cupric sulfate solutions at certain specific gravities. This research was an observational analytic study using a cross-sectional design. One mL of venous blood sample was consecutively collected from thirty donors who came to the blood service of the Dr. R. Soedjono Selong Hospital in August 2017. Next, screening for hemoglobin levels was carried out on those blood samples using the cupric sulfate method with a specific gravity of 1.054. Autoanalyzer was also used as a reference method in this research. Results of cupric sulfate examination were categorized into sinking and non-sinking. The results of cupric sulfate examination with sinking category showed significant suitability with hemoglobin levels of >12.5 g/dL from autoanalyzer (p=0.002) with 100% sensitivity and 70.83% specificity. The results of cupric sulfate examination with non-sinking category (with daily replacement) showed a significant match with hemoglobin levels of >12.5 g/dL from autoanalyzer (p=0.003) with 68% sensitivity and 100% specificity. The use of ten samples in this research could reach 100% sensitivity and 100% specificity. Meanwhile, according to the WHO, 30 mL of cupric sulfate solution with a specific gravity of 1.053 can accommodate 25 donor blood droplets.Thus, it can be concluded that unlike the reference from the WHO of 25 droplets, based on hemoglobin screening test cupric sulfate solution with a specific gravity of 1,054 can be saturated with ten droplets.
Comparison of Anti-Salmonella Typhi IgM Examination Results with SD Bioline and Tubex TF. Methods Herlinawati, Herlinawati; Hermawati, Resna; Adyana, I Gede Angga
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 7, No 3: September 2022
Publisher : Universitas Aisyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (592.202 KB) | DOI: 10.30604/jika.v7i3.1481

Abstract

Salmonella typhi is a gram-negative bacterium that is an intracellular facultative pathogen, which enters the human body and causes an acute systemic infectious disease called typhoid fever. Among the laboratory tests used to detect the presence of anti-Salmonella typhi IgM in serum are SD Bioline and Tubex TF. This study aims to determinecomparison of the results of the anti-Salmonella typhi IgM examination with the SD Bioline and Tubex TF methods. Analytical observation with cross-sectional design. This research was conducted in RSUD DR. Soedjono Selong. The time of the study was January-April 2022. The sample of this study was patients who underwent typhoid fever examination who were hospitalized at RSUD DR. Soedjono Selong as many as 60 people. This research uses the methodconsecutive sampling,Anti-Salmonella typhi IgM was examined with the SD Bioline kit using the immunochromatography method and the TUBEX TF kit using the Inhibition Magnetic Binding Immunoassay (IMBI) method. Data analysis was performed using SPSS, namely univariate test, and bivariate test with Chi-square. There were 26 (43.3%) male and 34 (56.7%) female patients. The youngest is 2 years old and the oldest is 64 years old with an average age of 25 years ± 19 years. Of the total 60 patients examined with SD Bioline, 5 (8.3%) were positive and 55 (91.7%) were negative. Meanwhile, examination with TUBEX TF found 3 (5%) positive people and 57 (95%) negative people. And there was a significant comparison between the results of the SD Bioline and Tubex TF examination with a value (p = 0.000) and a low level of conformity was obtained with a Kappa value of 0.000. A comparison of the results of the IgM anti-Salmonella typhi examination was obtained using the SD Bioline and Tubex TF . methodswith a very low degree of conformity.
The Relationship between TNF- Levels and Platelet Counts in Dengue Hemorrhagic Fever Patients in RSUD DR. Soedjono Selong, East Lombok Regency, West Nusa Tenggara Province Herlinawati, Herlinawati; Hermawati, Resna; Azmi, Fahriana; Sabariah, Sabariah
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 6, No 4: December 2021
Publisher : Universitas Aisyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (602.817 KB) | DOI: 10.30604/jika.v6i4.1479

Abstract

Dengue hemorrhagic fever is an infectious disease caused by the dengue virus (DENV) and transmitted through the Aedes aegypti mosquito. Macrophage infection by the dengue virus causes activation of T-helper and T-cytotoxic so that lymphokines and interferon-gamma are produced. Interferon-gamma will activate monocytes so that various inflammatory mediators are secreted such as TNF-, IL-1, and IL-6 which results in endothelial cell dysfunction and plasma leakage. Vascular disorders that occur are characterized by a decrease in the number of platelets. This study aims to determine the relationship between TNF- levels and the number of platelets in patients with dengue hemorrhagic fever at RSUD DR. Soedjono Selong, East Lombok Regency, NTB Province. Analytical observation with cross-sectional design. This research was conducted in RSUD DR. Soedjono Selong; the time of the study was April-September 2021. The sample of this study was dengue hemorrhagic fever patients who were hospitalized at RSUD DR. Soedjono Selong as many as 70 people. This research uses the method of consecutive sampling, with the independent variable in the form of TNF- and the dependent variable in the form of platelets. The platelet count was checked withThe Sysmex XN 550 autoanalyzer uses the hydrodynamic impedance counting method (sheath flow DC method) and checks the levels of TNF-? with an ELISA kit. Data analysis was carried out using SPSS, namely univariate test, and bivariate test with Chi-square. There were 27 (38.6%) males and 43 (61.4%) females with a total of 70 patients. The age of the youngest patient was 1 year and the age of the oldest patient was 66 years with a mean age of 24 years ± 20 years. All 70 dengue hemorrhagic fever patients had normal TNF- levels. There were 30 (48.6%) with normal platelet counts and 36 (51.4%) with abnormal platelet counts. And found a non-significant relationship between levels of TNF- with the number of platelets with a value (p = 0.908). There is no relationship between levels of TNF- with platelet count in dengue hemorrhagic fever patients in RSUD DR. Soedjono Selong, East Lombok Regency, NTB Province.
RELATIONSHIP BETWEEN SPECIFIC GRAVITY OF CUPRIC SULFATE AND SATURATION OF BLOOD DROPLETS DURING DONORS'HEMOGLOBIN SCREENING Resna Hermawati; Solichul Hadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 25 No. 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1376

Abstract

There are several methods of hemoglobin screening. A technique that is practical, easy, and fast,as well as does not require high costs is needed for blood donor activities. Cupric sulfate method is still used in blood donor activities nowadays. There are several types of the specific gravity of cupric sulfate which will determine saturation speed. On the other hand, one of the requirements for a blood donor, according to the WHO, is that its hemoglobin level must be ≥ 12.5 g/dL. This research aims was to reveal how many blood droplets could be dripped into cupric sulfate solutions at certain specific gravities. This research was an observational analytic study using a cross-sectional design. One mL of venous blood sample was consecutively collected from thirty donors who came to the blood service of the Dr. R. Soedjono Selong Hospital in August 2017. Next, screening for hemoglobin levels was carried out on those blood samples using the cupric sulfate method with a specific gravity of 1.054. Autoanalyzer was also used as a reference method in this research. Results of cupric sulfate examination were categorized into sinking and non-sinking. The results of cupric sulfate examination with sinking category showed significant suitability with hemoglobin levels of >12.5 g/dL from autoanalyzer (p=0.002) with 100% sensitivity and 70.83% specificity. The results of cupric sulfate examination with non-sinking category (with daily replacement) showed a significant match with hemoglobin levels of >12.5 g/dL from autoanalyzer (p=0.003) with 68% sensitivity and 100% specificity. The use of ten samples in this research could reach 100% sensitivity and 100% specificity. Meanwhile, according to the WHO, 30 mL of cupric sulfate solution with a specific gravity of 1.053 can accommodate 25 donor blood droplets.Thus, it can be concluded that unlike the reference from the WHO of 25 droplets, based on hemoglobin screening test cupric sulfate solution with a specific gravity of 1,054 can be saturated with ten droplets.
Co-Authors Adyana, I Gede Angga Azmi, Fahriana Herlinawati Herlinawati Paulus Budiono Notopuro Sabariah Sabariah Solichul Hadi Solichul Hadi