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Nisa', Khairun
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Chemical Engineering, Chemistry & Bioengineering Chemistry

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Isolasi, Karakterisasi dan Amobilisasi α-Amilase dari Aspergillus niger FNCC 6018 Nisa', Khairun
Chem Info Journal Vol 1, No 1 (2013)
Publisher : Chem Info Journal

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Abstract

Aspergillus niger FNCC 6018. The purposes of this research are to get specific activity data of isolated α-amylase from Aspergillus niger FNCC 6018, get optimum condition of enzyme data, include: pH and temperature, and get activity data of immobilized α-amylase in matrix bacto agar and recurrence test. The method is use in this research to determine α-amylase activity test by measuring maltose that produce from α-amylase which hydrolize amylose with Bernfeld method using DNS (3,5-dinitrosalicylic acid). Amount of protein was measured by Lowry method using standard BSA (Bovine Serum Albumin). The result of this research show that α-amylase enzyme isolated from Aspergillus niger FNCC 6018 which ammonium sulphate fraction 3 (40-60%) has the highest specific activity amount 5.7 Unit/mg protein. Optimum condition α-amylase from Aspergillus niger FNCC 6018 was achieved at optimum pH value 5.0 and temperature 36οC. Immobilized α-amylase in matrix bacto agar had specific activity amount 1.95 Unit/mg protein and use until twice recurrence.
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