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SALIVARY PROFILE AND IDENTIFICATION OF EXISTING CANDIDA sp OF AN INSTITUTIONALIZED ELDERLY POPULATION IN JAKARTA: PROFIL SALIVA DAN IDENTIFIKASI KEBERADAAN CANDIDA sp PADA LANSIA DI PANTI JOMPO DI JAKARTA Febrina Rahmayanti; Yuniardini Septorini Wimardhani; Indriasti Wardhany
Dentika: Dental Journal Vol. 17 No. 4 (2013): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.57 KB) | DOI: 10.32734/dentika.v17i4.1783

Abstract

Saliva plays an important role to protect oral and dental health, in maintenance oral microflora balance. In elderly, disease, and medications usually influence the saliva. The purpose of this preliminary study was to investigate salivary profile, oral symptoms of dryness, and existing of Candida sp of an institutionalized elderly in Jakarta, Twenty seven (27) elderly (17 men and 10 women) were examined in a cross sectional study. Unstimulated whole saliva was collected for 5 minutes, according to published protocol, and then salivary flow rate and pH were measured. Candida sp were cultured in CHROM agar. The yeasts were counted and identified. Oral symptom or complaints of oral dryness, and burning or itching were recorded with an interview. Data of systemic conditions and medication the used were obtained from the medical record. Eighteen (66.7%) CHROM agar plates were Candida sp positive cultured, and about 61.1% subjects’ salivary pH were below normal range. Twenty one (77.8%) subjects had oral symptom and complain about oral dryness, even thought the salivary flow rate were in normal range. In this study, C.albicans is not the most frequently found colonized. About 72% were identified not C.albicans. This study suggests that, in elderly which salivary pH was below normal range have greater opportunistic infected by Candida sp. It can be concluded that, the result did not confirm a significant difference between subjects’ data and salivary profile concentration. The mycological finding Candida albicans is not the most frequently found colonized in this elderly population. Further study which lagger elderly population and might give deeper insights of salivary profile in this population.
RELATIONSHIP OF SALIVARY PEROXIDASE ACTIVITY TO CD4+ LEVEL IN HIV/AIDS PATIENTS: HUBUNGAN AKTIVITAS PEROKSIDASE SALIVA TERHADAP TINGKAT CD4 PASIEN HIV/AIDS Febrina Rahmayanti; Yuniardini S Wimardhani
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.077 KB) | DOI: 10.32734/dentika.v16i1.1904

Abstract

Human whole saliva contains peroxidases (human peroxidase and myeloperoxidase) which are part of the innate host defence mechanism in oral cavity. The primary function is to catalyse the oxidation of thyocyanate, in the presence of hydrogen peroxidase, that resulting in the end product of wide antimicrobial activity. Patient with HIV/AIDS is often decreased in salivary flow rate, and this condition may also change the salivary composisition. These changes are contributed in oral lesions of HIV/AIDS patients. The aim of this study was to evaluate the salivary peroxidase activities of HIV/AIDS patients. The study involved 98 patients with HIV/AIDS of whom 55 were men and 43 were women. Unstimulated whole saliva was collected and all the patients received a complete oral examination. The saliva samples were analysed with Bradford assay (with Bradford reagent) and salivary peroxidase measurement with microplate reader and read at 450 nm wave length. The results showed that the mean concentration of total salivary protein and peroxidase in this study population was 1169µg/ml (SD±186.1) and 6.101unit/mg Protein (SD±1.26) respectively. It showed a tendency that the lower the CD4 level, the higher the total protein concentration as well as salivary peroxidase activity, however the difference was not statistically significant (p>0.05). Patients’ data on age, sex, salivary flow rate and pH did not significantly influencing the total salivary protein concentration as well as the peroxidase activity (p>0.05). This study showed that salivary peroxidase activity have a correlation with the total salivary protein concentration. There was a tendency of higher total protein concentration and salivary peroxidase activity found in patients with lower CD4 counts but in those with more acidic saliva. In conclusion, salivary peroxidase activity have a correlation with the total salivary protein concentration in HIV/AIDS patients.
IDENTIFIKASI FAKTOR RISIKO TERJADINYA KOLONISASI SPESIES CANDIDA DI RONGGA MULUT PADA INDIVIDU SEHAT (PENELITIAN PADA MAHASISWA & MAHASISWI FKG UI) Audiawati Audiawati; Siti Aliyah Pradono; Febrina Rahmayanti
Majalah Sainstekes Vol 2, No 1 (2015): Juni 2015
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.973 KB) | DOI: 10.33476/ms.v2i1.1638

Abstract

Introduction : Cases of oral candidiasis are commonly found, both in healthy individuals and immunecompromise patients, however publications of Candida carrier in the oral cavity of healthy population and risk factors for colonization in Indonesia are hardly available. Objective : This study was aimed to analyze the type and number of Candida colonies and identify risk factors in the oral cavity of apparenthly health FKG UI students. Material and methods : the specimens were taken from 195 subjects with oral rinse technique for identification using culture medium CHROMagar® and Sabaraoud dextrose agar. Results and discussion : Candida species were found in the 107 subjects oral cavity (54.87%), being Candida albicans was is the predominant species (52.33%). Some 88 subjects (82.24%) was dominant in the number of colonies less than 400 CFU/ml, while the rest had colony of more than 400 CFU/ml (17.76%). Candida colony grew dominantly in single colony (90.65%), and the others showed multi-species colonies (9.34%). Risk factors identified included age; gender; hormonal; blood type O; denture; orthodontic appliances; unstimulated salivary flow; pH of saliva; smoking, alcohol and oral cleaning habit; and oral health status. By using a statistical Pearson chi-square test, no  significant relationship was found between risk factors and number of Candida colonies in the oral cavity p less than 0.05. Conclusion : there was no one single risk factor for Candida colonization, but  combination of various risk factors for demographis, local and systemic was observed