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Ondho, Y. S.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Level of sodium chloride (NaCl) and profile of cervical mucus of dairy cattle at various age synchronized by prostaglandine Ondho, Y. S.; Akbar, F. A.; Lestari, D. A.; Samsudewa, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 4 (2019): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.4.364-371

Abstract

Objectives of this study were to compare the percentage of Sodium Chloride (NaCl) and cervical mucus profile which includes potential of Hydrogen (pH), abundance of cervical mucus, spinnbarkeit and ferning of dairy cattle at different age during estrus cycle. Thirty Two head of dairy cattle were used as materials which are 2 years old (n = 2), 3 years old (n = 18), 4 years old (n = 8) and 5 years old (n=4). Dairy cattle were estrus synchronized using 50 mg/head of prostaglandin. Data was collected on 48, 72, 78, 84, 90, 120, 144 and 408 hours after prostaglandin administration. The data were analyzed by using non parametric statistic which was Kruskal-Wallis H test. The significant data was tested with Mann-Whitney U test. The result showed that the abundance of cervical mucus was significantly different (P<0.05; χ2 = 0.011) on 84 hours after estrus synchronization. However, level of NaCl, abundance, spinnbarkeit and ferning of cervical mucus was not sigificantly different. In conclusion, the age of dairy cattle did not affected to the condition NaCl percentage, spinnbarkeit, pH and ferning of cervical mucus, but affected to the abundance of cervical mucus on 84 hours after adminitration of prostaglandine.
Gonadal hormones level and morphometric traits in cow deliveres twin and single calves Yuwono, T.; Sumeidiana, I.; Ondho, Y. S.; Kurnianto, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 2 (2017): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.2.128-132

Abstract

The objectives of study were to investigate follicle stimulating hormone (FSH) and luteinizing hormone (LH) level in four groups beef cow composed of Ongole Grade cow delivered twinning calves (OGT), Ongole Grade cow delivered single calf (OGS), Simmental crossbred cow delivered twinning calves (SCT) and Simmental crossbred cow delivered single calf (SCS). Morphometric traits included body weight, heart girth, body length, shoulder height, and abdominal circumference were taken from cows. Cows were reared by farmers in Grobogan and Kendal Regency, Central Java. Blood serums of cows were taken at estrus. FSH and LH concentration were measured with enzyme linked fluorescent assays (ELFA) technique.FSH and LH concentration were compared between OGT, OGS, SCT, and SCS. Result showed that there were no significant difference of FSH and LH concentration between OGT and OGS, between OGT and SCT, between OGT and SCS. There were no significant difference between OGS and SCT, and OGS and SCS. There were no significant difference between SCT and SCS. Low concentration of FSH and LH in all cows was related to suckle period, when prolactin concentration was inversely related to FSH and LH concentration.
Modeling for determining the superiority of Holstein bulls as frozen semen producer and genetic source for milk production Argiris, A.; Santoso, S. I.; Ondho, Y. S.; Kurnianto, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.1.47-55

Abstract

The objective of this study was to develop models for determination the superiority of Holstein bulls as a producer of frozen semen and inheritance of the genetic traits of milk production. The ability of the bull to produce frozen semen per years was analyzed descriptively. Reproductive efficiency of frozen semen in artificial insemination was calculated by service per conception (S/C). Estimation sire breeding value for milk production was calculated by contemporary comparison (CC) method. Model of superiority bulls was analyzed by Structural Equation Model with Partial Least Square method (SEM-PLS). Total average production of frozen semen was 23,109±14,970 doses/year. The average S/C was 2.83. The CC value ranged from -1,865.7 until +1,636.3. Potency of milk production resulted from lactation cow offspring per bulls ranged from 951,749.2 to 52,347,822.9 liters per year. The economic value of bulls based on the potency milk production of offspring ranges from IDR 4,758,745,999 to IDR 261,739,114,505. The superiority of bulls was affected significantly (P<0.05) by frozen semen production, reproductive efficiency and average milk production of daughter cows (DC) as much as 0.59, -0.53 and 0.33, respectively. In conclusion, the superiority of bull can be explained about 78.3% by the production of frozen semen production, reproductive efficiency and milk production of offspring.
Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center Mahendra, H. C.; Samsudewa, D.; Ondho, Y. S.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 1 (2018): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.1.26-34

Abstract

This study aimed to evaluate and compare the quality of post thawing spermatozoa of buffalo frozen semen produced by artificial insemination centers on standard values, and proposed reference values (PRV). Materials of the research were 60 samples of straws obtained from three Artificial Insemination Center, which are each 20 straws, respectively. Parameters observed were motility, concentration, longevity, plasma membrane integrity (PMI), acrosome integrity (AIn) and recovery rate. The obtained data were tested by Z test then presented as mean ± standard deviation. The research results from three artificial insemination center showed that motility mean was 45.00±3.07%, concentration mean was 26.09±3.11 x106 cells/0.25mL, longevity mean was 10.38±0.75%/hour, PMI mean was 45.86±10.67%, AI mean was 73.99±5.28% and recovery rate mean was 64.38±5.16%. The conclusions of this research were the motility and concentration mean were higher than Indonesian National Standard (INS), longevity mean was lower than Department of Animal Husbandry, Dairying and Fisheries (DADF) standard but PMI and AIn mean were higher, the mean value of recovery rate was higher than of proposal value. 
Co-Authors Akbar, F. A. Argiris, A. D. Samsudewa E. Kurnianto Lestari, D. A. Mahendra, H. C. S. I. Santoso, S. I. Sumeidiana, I. T. Yuwono