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All Journal Folia Medica Indonesiana BIOMA Biopendix: Jurnal Biologi, Pendidikan dan Terapan Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat BIOEDUPAT: Pattimura Journal of Biology and Learning PAMERI : Pattimura Medical Review Scientica: Jurnal Ilmiah Sains dan Teknologi
Warella, Juen Carla
Department Of Microbiology And Parasitology, Faculty Of Medicine, Universitas Pattimura, Ambon, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Other

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LAMA FERMENTASI TERHADAP KADAR SERAT NATA BUAH GANDARIA Juen Carla Warella; Pamella Mercy Papilaya; Prelly Tuapattinaya
BIOPENDIX Vol 3 No 1 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue1page33-39

Abstract

Background: Gandaria fruit on the island of Ambon has benefits if the sale value can be used as raw material for the manufacture of Nata products. The making of Nata Gandaria fruit with the help of Acetobacter xylinum microorganisms, the role of microbes to break down glucose into cellulose or fiber that is important for the human body. Methods: To know the length of fermentation to know the effect of fiber content of Gandaria nata, then tested on fiber content of nata Gandaria fruit that has been fermented for 10 days and 12 days. Result: Based on the test results using gravimetry fiber content method known nata fermentation time can increase fiber content. The highest increasing fiber content at nata occurred on day 12 with an average fiber content of 0.45% and the lowest occurred on fermentation day 10 with an average fiber content of 0.35%. Conclusion: The duration of fermentation has an effect on the fiber content of gandaria fruit (Bouea macrophylla griff). The duration of fermentation that produces gandaria fruit nata (Bouea macrophylla griff) with the highest fiber content is on the 12th day while the fermentation time that produces the gandaria fruit (Bouea macrophylla griff) with the least fiber content is the 10th day
ANALISIS KADAR AIR DAN KADAR ABU TEH BERBAHAN DASAR DAUN LAMUN ( Enhalus acoroides) Prelly M Tuapattinaya; Rufiati Simal; Juen Carla Warella
BIOPENDIX Vol 8 No 1 (2021): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol8issue1page16-21

Abstract

Background: Seagrass is one of the components that make up this diversity. In addition to fruit and seeds, other parts of the seagrass plant, Enhalus acoroides, the opportunity to utilize seagrass as an alternative food base is very large and has the potential to be processed into innovative foods such as herbal teas. Methods: Testing the Moisture Content by means of a porcelain cup dried in an oven at 105˚C for 3 hours, after that it was weighed, added as much as 100g of samples of Seagrass tea extract (Enhalus acoroides) and placed in an oven at 105˚C for 3 hours. The weight of the cup and sample was weighed. The ash content was tested in the same way, except that 1-2 g of the sample was used and then dried in an electric furnace at 600˚C for 18-24 hours. Samples that have become ash are weighed. Results: The results of the analysis of the water content of seagrass tea (Enhalus acoroides) obtained the value of the water content of seagrass tea ranging from 9.17%-9.18%. The average value of the water content of seagrass tea is 9.17%. The results of the analysis of the ash content of seagrass tea (Enhalus acoroides) obtained that the water content of seagrass tea ranged from 18.52%-18.56%. The average value of the water content of seagrass tea is 18.53%. Conclusion: From the data obtained, it can be concluded that the water content of Seagrass Tea (Enhalus acoroides) is 9.17% and the Ash content of Seagrass Tea (Enhalus acoroides) is 18.53%.
Organoleptic Test of Eco-enzyme : Fermentation of Banana Peel Waste Juen Carla Warella
Bioma : Jurnal Ilmiah Biologi Vol 12, No 1: April 2023
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v12i1.14803

Abstract

Prevention of environmental damage can be done by recycling household waste both organic and inorganic. One of them is by making eco-enzymes made from banana peel waste which is found in the environment. This study aims to determine the level of respondents' liking based on organoleptic test variables. This research method is an experiment that includes making eco-enzymes, and organoleptic tests consisting of aroma, color and texture variables. Data analysis was carried out in a qualitative descriptive way by looking at the level of respondents' liking. The results showed that the average respondent chose a brown color with a percentage of 90%, yellow 7%, and colorless 3%. The scent variable respondents chose sour odor by 70% and other aromas 30%. While the texture of all respondents chose the composition of liquid eco-enzyme. This is due to the metabolic activity of microorganisms that result in the breakdown of substrates by bacteria resulting in changes in the aroma, color and texture of banana peels. The conclusion of this study is that the use of banana peel as the basic ingredient for making eco-enzyme affects the respondents' level of liking. Eco-enzyme can be used as a natural fertilizer for plant growth and can reduce household waste.
ANALISIS KADAR KARBOHIDRAT PADA TEH CELUP BERBAHAN DASAR DAUN LAMUN (Enhalus acoroides) Prelly Marsell Jolanda Tuapattinaya; Ferymon Mahulette; Juen Carla Warella; Rufiati Simal; Nopri Ratusehaka
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 9 No 2 (2023): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol9issue2page232-236

Abstract

Background: Seagrass is the only flowering plant (angiosperm) that has true root and leaf rhizomes that live submerged in the sea. There are 60 species of seagrass scattered in the world's coastal waters, one type of seagrass that can be used by humans is the seagrass Enhalus acoroides. The economic advantage of Enhalus acoroides leaves can be used as a food ingredient because it has a complete and relatively high nutritional content. Enhalus acoroides leaves can be used as raw material for herbal teas. Herbal teas have a high carbohydrate content, for example in Camellia sinensis tea of ​​0.286%, carbohydrates are one of the chemicals that are needed by the human body. Methods: This study was a descriptive study to determine the value of carbohydrate content in seagrass (Enhalus acoroides) leaf teabags. The research was carried out in March 2022. Sampling of seagrass leaves was carried out in Suli Village, Salahutu District, Central Maluku Regency. The stage of making seagrass teabags was carried out at the Basic Biology Laboratory of FKIP Pattimura University. And the analysis of carbohydrate content was carried out at the Biochemistry Laboratory of the Faculty of Mathematics and Natural Sciences, Pattimura University, Ambon with the type of experimental research. Results: Based on the observations that have been made, the results showed that the carbohydrate content of TL U1 was 5.148%, TL U2 was 5.211%l, TL U3 was 5.165%. With an average carbohydrate of 5.174%. Conclusion: Seagrass leaf teabags (Enhalus acoroides) have a high carbohydrate content and can be a new product that is rich in nutrients.
Antifungal Activity of Selaginella plana (Desv. ex Poir.) Hieron Extract Against Candida albicans In Vitro Juen Carla Warella; Khairunnida Rahma; Agung Dwi Wahyu Widodo; Rebekah Juniati Setiabudi
Folia Medica Indonesiana Vol. 59 No. 3 (2023): September
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v59i3.44165

Abstract

Highlights: 1. A study on the medical benefits of Selaginella plana has significant academic value due to its extensive traditional usage among the Moluccan people as a medicinal remedy, especially for its antifungal properties.2. The findings of this study will allow further screening to determine the mechanism of action of bioactive compounds in inhibiting the growth of Candida albicans   Abstract Candidiasis is an opportunistic infection caused by Candida albicans. This infection commonly affects the skin, oral mucosa, vagina, and gastrointestinal tract. Excessive use of azole antifungals in the treatment of Candida albicans infections can lead to the development of resistance. Therefore, it is necessary to explore alternative treatments using medicinal plants such as Selaginella plana, commonly referred to as “rutu-rutu” in a local language spoken across Maluku, Indonesia. Selaginella plana contains active compounds belonging to various chemical classes, including terpenoids, steroids, flavonoids, and saponins. This study aimed to determine the ability of Selaginella plana extract as an antifungal agent against Candida albicans by evaluating its inhibitory and antifungal effects. This study used an actual experimental design and broth dilution method. The research methodology involved the extraction of Selaginella plana using a solvent of 96% ethanol. The extract was then prepared in various concentrations, i.e., 100%, 50%, 25%, 12.5%, 6.25%, and 3.125%. Additionally, ketoconazole and distilled water were included in the experiment for the positive and negative controls, respectively. The results of this study demonstrated that Selaginella plana extract inhibited the growth of Candida albicans when administered starting at a concentration of 12.5%. However, the antifungal potential of Selaginella plana extract that induced cell death was only observed at a concentration of 100%. The fungicidal activity was exclusively identified in the undiluted, pure extract. The inhibitory and cytotoxic effects of Selaginella plana on Candida albicans cells were attributed to the presence of bioactive compounds in Selaginella plana, including flavonoids, tannins, terpenoids, and saponins. These bioactive compounds had the ability to inhibit cell growth by altering membrane permeability, causing mitochondrial dysfunction, and disrupting ergosterol biosynthesis. It can be concluded that Selaginella plana extract can act as a fungistatic agent against the proliferation of Candida albicans.
Effect of calcium carbide on the rate of ripening of Ambon Bananas (Musa paradisiaca Var. Sapientum (L). Kunt) Jeyssen Lincon Loupatty; Gloria Eka Sari Tuhumena; Gysella Velove Limba; Ayu Wariunsore; Zipora V Noya Van Delzen; Juen Carla Warella; Gerce Anne Rumahlatu
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 3 No 2 (2023): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v3.i2.pp118-124

Abstract

Bananas are one of the most widely consumed sources of nutrients in Indonesia. Generally, bananas are consumed fresh and processed into various snacks. This leads to an increased demand for bananas in the market. However, the natural ripening process takes 7-8 days after harvest, which is a long time to meet consumer demand. One solution is to use calcium carbide to accelerate fruit ripening. The reason for using calcium carbide is that it is readily available and inexpensive, so banana producers use carbide as a fruit ripening agent. The objective of this study was to determine the effects of calcium carbide on fruit ripening of Ambon bananas. The method used was a laboratory experiment with a post-test-only control group design. There were three treatments with calcium carbide concentrations of 12%, 6%, and 3%, and positive control, namely rapining with rice. Data were analyzed descriptive qualitative by collecting, presenting, reducing, and conclusions. The process of fruit ripening can be observed using the variables of color, texture and aroma. The results obtained were differences in color, texture, and aroma at concentrations of 12%, 6%, and 3% based on the length of aging. Based on the color parameter, the concentration of 6% and 12% showed a blackish yellow/brown color, while the concentration of 3% was yellow with black spots. The texture parameters of the 6% and 12% concentrations had a very soft texture, while the 3% concentration had a smooth texture. The aroma parameters of the 12%, 6%, and 3% concentrations had a rancid aroma on day six, while days one, two, and three were fragrant. The conclusion is that using calcium carbide at different concentrations affects the rate of fruit ripening. The higher the concentration of calcium carbide used, the faster the respiration rate of the bananas, thus initiating the ripening process
KEJADIAN GASTROESOPHAGEAL REFLUX DISEASE (GERD) BERDASARKAN SKOR GERD-Q PADA MAHASISWA FAKULTAS KEDOKTERAN UNIVERSITAS PATTIMURA TAHUN 2023 Nurul Fadila Rahmadani Maradjabessy; Indrawanti Kusadhiani; Juen Carla Warella
PAMERI Vol 5 No 2 (2023): PAMERI: Pattimura Medical Review
Publisher : Fakultas Kedokteran Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pamerivol5issue2page76-84

Abstract

Gastroesophageal Reflux Disease (GERD) is a pathological condition due to backflow from the stomach into the esophagus, causing symptoms such as heartburn and regurgitation. Risk factors for GERD are mostly related to a person's lifestyle such as obesity, diet, smoking and stress. One group of individuals who have a high risk is students of the Faculty of Medicine in terms of a series of study loads that make students unable to maintain a good lifestyle. The purpose of this study was to determine the incidence and characteristics of Pattimura University Faculty of Medicine students who experience GERD based on the GERD-Q score. This research is a quantitative research with categorical descriptive study using cross sectional approach. The results showed that the prevalence of GERD was 40 people (15.2%) out of a total of 264 respondents. The majority were female 31 people (11.7%). The highest age was 18 years old, 16 people (6.1%). The most common symptom of GERD is regurgitation as many as 40 people (8.5%). The highest BMI in the normal group was 16 people (6.1%). The conclusion of this study showed that the prevalence of GERD in students of the Faculty of Medicine, Pattimura University was 40 people with the main complaint of regurgitation and the characteristics of respondents with GERD were mostly at the age of 18 years, female, and normal BMI group.
PERTUMBUHAN BIOFILM ESCHERICHIA COLI PADA MEDIA TRYPTIC SOY BROTH Khairunnida Rahma; Juen Carla Warella
Scientica: Jurnal Ilmiah Sains dan Teknologi Vol. 2 No. 4 (2024): Scientica: Jurnal Ilmiah Sains dan Teknologi
Publisher : Komunitas Menulis dan Meneliti (Kolibi)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.572349/scientica.v2i4.1214

Abstract

Mikroorganisme mengembangkan berbagai tipe mekanisme pertahanan yang berbeda-beda. Salah satunya adalah pembentukan biofilm. Biofilm merupakan kumpulan dari mikroorganisme terkait permukaan yang heterogen terselubuh di dalam matriks polimer yang diproduksi sendiri yang terdiri dari polisakarida, protein, dan DNA. Tujuan penelitian ini adalah untuk menganalisis pertumbuhan biofilm bakteri Gram-negatif Escherichia coli pada media Tryptic Soy Broth. Metode yang digunakan adalah microtiter-plate 96 wells. Dilakukan sebanyak enam kali replikasi dan pembacaan Optical Density (OD) menggunakan ELISA reader pada Panjang gelombang 570 nm. Hasil penelitian didapatkan rerata OD 2,810 dengan standar deviasi 0,402. Sehingga dapat ditarik kesimpulan, Escherichia coli termasuk ke dalam kategori bakteri pembentuk biofilm yang kuat.
Peningkatan Level Pengetahuan Masyarakat Mengenai Manfaat Pala Sebagai Tanaman Rempah Obat (TARO) Melda Yunita; Morgan Ohiwal; Saiful Alimudi; Eka Astuty; Rosdiana Mus; Muthmainnah Abbas; Miftah H Makatita; Juen C Warella
Darmabakti : Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol 5 No 01 (2024): Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat
Publisher : Lembaga Peneliian dan Pengabdian Masyarakat (LPPM) Universitas Islam Madura (UIM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31102/darmabakti.2024.5.01.27-34

Abstract

Tanaman pala (Myristica fragrans Houtt) memiliki nilai ekonomis yang tinggi dan multiguna. Selain digunakan sebagai bumbu masak, pala juga memiliki banyak manfaat dalam bidang kesehatan sehingga dapat dijadikan sebagai tanaman rempah obat (TARO). Namun, pemanfaatan pala sebagai tanaman obat belum begitu maksimal di Dusun Air Sakula Negeri Laha Ambon. Padahal, kebanyakan masyarakat disana bekerja sebagai petani pala. Tujuan dari kegiatan ini adalah untuk menganalisis level pengetahuan masyarakat mengenai pemanfaatan tanaman pala sebagai tanaman rempah obat. Kegiatan dilaksanakan pada tanggal 13 Agustus 2022 di Dusun Air Sakula Negeri Laha Ambon dengan melibatkan 20 responden. Tahapan yang dilakukan ialah melaksanakan pretest, penyuluhan TARO, dan posttest serta evaluasi. Hasil analisis menunjukkan bahwa level pengetahuan masyarakat meningkat sebesar 67% setelah mendapatkan penyuluhan. Dapat disimpulkan penyuluhan yang diberikan berdampak positif dan signifikan dalam meningkatkan level pengetahuan masyarakat mengenai pala sebagai tanaman rempah obat.
Co-Authors Agung Dwi Wahyu Widodo Ayu Wariunsore Eka Astuty Ferymon Mahulette Gerce Anne Rumahlatu Gloria Eka Sari Tuhumena Gysella Velove Limba Indrawanti Kusadhiani Jeyssen Lincon Loupatty Khairunnida Rahma Khairunnida Rahma Miftah H Makatita Mus, Rosdiana Muthmainnah Abbas Nopri Ratusehaka Nurul Fadila Rahmadani Maradjabessy Ohiwal, Morgan Pamella Mercy Papilaya Prelly M Tuapattinaya Prelly Marsell Jolanda Tuapattinaya Prelly Tuapattinaya Rebekah Juniati Setiabudi Rufiati Simal Saiful Alimudi Yunita, Melda Zipora V Noya Van Delzen