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All Journal Biospecies Jurnal technoscientia JURNAL TEKNOLOGI TECHNOSCIENTIA Jurnal Teknologi Lingkungan Lahan Basah Makara Journal of Science
Yuliarni, Floreta Fiska
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Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Earth & Planetary Sciences Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Public Health

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EKSTRAKSI JAMUR AURICULARIA DENGAN MENGGUNAKAN PELARUT ETANOL DAN METANOL Yuliarni, Floreta Fiska; Ayu Puji Lestari, Kinanti; Kun Arisawati, Diah; Dwi Winda Sari, Ratna; Ratna K., Kharisma
JURNAL TEKNOLOGI TECHNOSCIENTIA Technoscientia Vol 14 No 2 Maret 2022
Publisher : Lembaga Penelitian & Pengabdian Kepada Masyarakat (LPPM), IST AKPRIND Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34151/technoscientia.v14i2.3637

Abstract

The purpose of this study was to determine the characteristics of Auricularia extract which was extracted using 96% ethanol (sample E) and methanol (sample M). Auricularia was powdered before extraction. Extraction of mushrooms was carried out using the maceration method for three days. The solvents used for extraction were 96% ethanol and methanol. The extract that has been obtained is calculated by weight yield, tested for solubility, pH, and organoleptic tests. The yield weight of sample E was 1.47% higher than sample M, which was 1.22%. In the solubility test, sample E dissolved in water had a higher solubility of 60% compared to 96% ethanol, which was 55%, on the other hand, sample M dissolved in ethanol 96% had a higher solubility of 55% compared to 40% in water. Samples E and M dissolved in water had the same pH of 5, as well as those dissolved in 96% ethanol, which was 6. The organoleptic test on samples E and M had similarities in smell and shape, the difference was the color. The conclusion is samples E and M have almost the same characteristics.
EKSTRAKSI JAMUR AURICULARIA DENGAN MENGGUNAKAN PELARUT ETANOL DAN METANOL Yuliarni, Floreta Fiska; Ayu Puji Lestari, Kinanti; Kun Arisawati, Diah; Dwi Winda Sari, Ratna; Ratna K., Kharisma
JURNAL TEKNOLOGI TECHNOSCIENTIA Technoscientia Vol 14 No 2 Maret 2022
Publisher : Lembaga Penelitian & Pengabdian Kepada Masyarakat (LPPM), IST AKPRIND Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34151/technoscientia.v14i2.3637

Abstract

The purpose of this study was to determine the characteristics of Auricularia extract which was extracted using 96% ethanol (sample E) and methanol (sample M). Auricularia was powdered before extraction. Extraction of mushrooms was carried out using the maceration method for three days. The solvents used for extraction were 96% ethanol and methanol. The extract that has been obtained is calculated by weight yield, tested for solubility, pH, and organoleptic tests. The yield weight of sample E was 1.47% higher than sample M, which was 1.22%. In the solubility test, sample E dissolved in water had a higher solubility of 60% compared to 96% ethanol, which was 55%, on the other hand, sample M dissolved in ethanol 96% had a higher solubility of 55% compared to 40% in water. Samples E and M dissolved in water had the same pH of 5, as well as those dissolved in 96% ethanol, which was 6. The organoleptic test on samples E and M had similarities in smell and shape, the difference was the color. The conclusion is samples E and M have almost the same characteristics.
Multilocus Sequence Analysis of Cercospora spp. from Different Host Plant Families Yuliarni, Floreta Fiska; Sjamsuridzal, Wellyzar; Hidayat, Iman
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

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Abstract

Identification of the genus Cercospora is still complicated due to the host preferences often being used as the main criteria to propose a new name. We determined the relationship between host plants and multilocus sequence variations (ITS rDNA including 5.8S rDNA, elongation factor 1-α, and calmodulin) in Cercospora spp. to investigate the host specificity. We used 53 strains of Cercospora spp. infecting 12 plant families for phylogenetic analysis. The sequences of 23 strains of Cercospora spp. infecting the plant families of Asteraceae, Cucurbitaceae, and Solanaceae were determined in this study. The sequences of 30 strains of Cercospora spp. infecting the plant families of Fabaceae, Amaranthaceae, Apiaceae, Plumbaginaceae, Malvaceae, Cistaceae, Plantaginaceae, Lamiaceae, and Poaceae were obtained from GenBank. The molecular phylogenetic analysis revealed that the majority of Cercospora species lack host specificity, and only C. zinniicola, C. zeina, C. zeae-maydis, C. cocciniae, and C. mikaniicola were found to be host-specific. Closely related species of Cercospora could not be distinguished using molecular analyses of ITS, EF, and CAL gene regions. The topology of the phylogenetic tree based on the CAL gene showed a better topology and Cercospora species separation than the trees developed based on the ITS rDNA region or the EF gene.
Pemanfaatan Air Leri dan Air Mikroorganisme Lokal Sebagai Pengendali Rayap Tanah Surahmaida, Surahmaida; Yuliarni, Floreta Fiska
Jurnal Teknologi Lingkungan Lahan Basah Vol 12, No 1 (2024): Januari 2024
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jtllb.v12i1.73006

Abstract

Termites (Coptotermes curvignathus) are considered more dangerous than other termite species as they can cause significant damage to plantation and agricultural crops, with widespread distribution. The use of chemical pesticides to control this pest can lead to resistance and have negative impacts on the environment and human health. An environmentally friendly solution involves utilizing natural plants/substances as botanical insecticides, such as rice washing water and local microorganism (MOL) water. The aim of this research is to evaluate the effects of these two substances on termite population control and determine the optimal formulation that can act as a termite repellent. and to determine the optimal formulation as an anti-termite. The research methodology involves determining formulations of botanical insecticides, namely 100% rice washing water, 100% MOL water, 50% rice washing water : 50% MOL water, 75% rice washing water : 25% MOL water, 25% rice washing water : 75% MOL water. Each test box is filled 5 soldier termites and 20 worker termites, then provided with test papers that have been soaked in the predetermined formulation. Each treatment is repeated three times and observed every 24 hours for 72 hours. The research results indicate that all formulations uses did not have a significant impact on termite mortality. It can be concluded that the use of rice washing water and MOL water in the tested formulations is not effective as an insecticide to control termite.
Uji Daya Hambat Ekstrak Metanol Auricularia nigricans terhadap Candida spp.: Inhibitory Test of Methanol Extract of Auricularia nigricans Against Candida spp. Indrawati, Afina; Pertiwi, Amelya Dyah; Ayuningtyias, Audry Ridho; Subroto, Hillda Widianingtyas; Azizah, Mufidhatul Nur; Handayani, Tutik; Surahmaida, Surahmaida; Lestari, Kinanti Ayu Puji; Yuliarni, Floreta Fiska
Biospecies Vol. 16 No. 2 (2023): Juli 2023
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v16i2.27451

Abstract

Candida was a fungus that can cause candidiasis infection. Candidiasis could occur of the skin, nails, mucous membranes and the gastrointestinal. The study aims to examine whether Auricularia nigricans methanol extract has the ability to inhibit the growth of Candida albicans, C. glabrata, and C. parapsilosis. Soxhletation was used as the extraction method, and the inhibitory test method involved diffusion paper disk and wells methods. The concentration variations used were 0.2 g/ml, 0.25 g/ml and 0.3 g/ml with 10% DMSO as a negative control. The research results obtained were that no inhibition zones were formed at concentrations of 0.2 g/ml, 0.25 g/ml and 0.3 g/ml so that they were included in the inactive category. Keywords: Auricularia nigricans, Candida, soxhlet method, methanol extract, paper disc and wells method.   Abstrak Candida merupakan jamur golongan khamir yang dapat menyebabkan infeksi kandidiasis. Kandidiasis tersebut dapat terjadi pada kulit, kuku, membran mukosa dan saluran cerna. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak metanol Auricularia nigricans dalam menghambat pertumbuhan C. albicans, C. glabrata dan C. parapsilosis. Metode ekstraksi yang digunakan adalah sokletasi selama 10 jam dan metode uji daya hambat antifunginya secara difusi cakram dan sumuran. Variasi konsentrasi yang digunakan adalah 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml dengan DMSO 10% sebagai kontrol negatif. Hasil penelitian yang diperoleh adalah tidak terbentuknya zona hambat pada konsentrasi 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml sehingga termasuk dalam kategori tidak aktif. Kata kunci: Auricularia nigricans, Candida, metode sokletasi, ekstrak metanol, metode difusi cakram dan sumuran.
Co-Authors AYU PUJI LESTARI, KINANTI Ayuningtyias, Audry Ridho Azizah, Mufidhatul Nur Dwi Winda Sari, Ratna Iman Hidayat Indrawati, Afina Kun Arisawati, Diah Lestari, Kinanti Ayu Puji Pertiwi, Amelya Dyah Ratna K., Kharisma Subroto, Hillda Widianingtyas Surahmaida, Surahmaida Tutik Handayani, Tutik WELLYZAR SJAMSURIDZAL