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All Journal Indonesian Journal of Pharmaceutical and Clinical Research
Zulharmita
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Determination of Dexamethasone in Unregistered Herbal Weight Gain Using HPTLC-Densitometry Asra, Ridho; Zulharmita; Yuliatim, Nopitri
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 1 No. 2 (2018): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (829.05 KB) | DOI: 10.32734/idjpcr.v1i2.331

Abstract

A method was described for the simultaneous determination of dexamethasone in herbal weight gain. Three unregistered herbal weight gains (sample A, B, and C) were analyzed by using HPTLC-densitometry method. Samples were extracted as bases into methanol, separated by HPTLC silica gel 60 F254plate using chloroform: methanol (9:1) as mobile phase followed by densitometry measurement of its spot. The result showed that the detector response was linear for concentrations between 100-500 mg/mL (r =0.998). The limits of detection and quantitation were 9.19 mg/mL and 30.64 mg/mL, respectively. Dexamethasone contents from samples were analyzed. The result showed that two samples (sample A and B) were positively containing dexamethasone and the other one showed a negative result. The average contents of dexamethasone from both samples were 0.23% and 0.25%, respectively.
Co-Authors Asra, Ridho Yuliatim, Nopitri