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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology KAUDERNI
Dewanto, Didit Kustantio
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Medicine & Pharmacology

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AKTIVITAS ANTIBAKTERI DARI EKSTRAK JAMUR LAUT Fadilah, Nur; Erawati, Erawati; Nahar, Hendrik; Dewanto, Didit Kustantio
KAUDERNI : Journal of Fisheries, Marine and Aquatic Science Vol 1 No 1 (2016)
Publisher : Sekolah Tinggi Perikanan dan Kelautan (STPL) Palu

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Abstract

A search new antibacterial compounds should continue to be done as a raw material of antibiotics. The purpose of this study, which is to get kind of marine fungi antibacterial activity and get the value of MIC and MBC. The study was conducted from the sampling sponge, marine fungi isolation, extraction, preparation of test bacteria, antibacterial activity testing and analysis of MIC and MBC. Data was analyzed by measuring the diameter of inhibition zone are formed. The results obtained, that debris ethanol fraction from marine fungi J3 can inhibit the growth of E. coli and S. aureus at a concentration of 100 mg/L to 100.000 mg/L. At the debris ethyl acetate fraction able to inhibit concentration of 1.000 mg/L to 100.000 mg/L. At the filtrate ethyl acetate fraction was able to inhibit 10.000 mg/L to 100.000 mg/L. For both types of test bacteria, MIC and MBC values are indicated by the highest debris ethanol fraction and lowest by the fraction of ethyl acetate filtrate.
KEPADATAN ASCIDIACEA (DIDEMNUM MOLLE) DI PERAIRAN PANTAI TASIK RIA, SULAWESI UTARA Dewanto, Didit Kustantio
KAUDERNI : Journal of Fisheries, Marine and Aquatic Science Vol 1 No 1 (2016)
Publisher : Sekolah Tinggi Perikanan dan Kelautan (STPL) Palu

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Abstract

This research was conducted with the aim of knowing the density of Ascidiacea D. molle located in Tasik Ria, Manado Bay, North Sulawesi. Collecting data and samples using diving equipment (SCUBA) with quadrant and transect method, placed in the 50 meters parallel to the shoreline. The first transect placed at a depth of 5 meters and second transect at a depth of 3 meters. In calculating the density of D. molle using a quadrant with a size of 50 x 50 cm placed on a transect at ten points. Average density D. molle at a depth of 5 meters higher than at a depth of 3 meters and vary in each month. This trend is strongly influenced by various environmental factors are not yet known, so it takes a period of time longer observation with an emphasis on the relationship between environmental factors and the frequency of  attendance D. molle.
POTENTIAL MARINE-DERIVED FUNGI ISOLATED FROM SPONGE IN PRODUCE NEW AND BENEFICIAL COMPOUNDS Tanod, Wendy Alexander; Muliadin, Muliadin; Adel, Yeldi S; Dewanto, Didit Kustantio
KAUDERNI : Journal of Fisheries, Marine and Aquatic Science Vol 2 No 1 (2020)
Publisher : Sekolah Tinggi Perikanan dan Kelautan (STPL) Palu

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Abstract

Marine organisms are a source of natural products and produce compounds with a molecular structure that is unique and useful. Thousands of new compounds have biological activitity for anticancer, antiviral, and antimicrobial. This compounds isolated from various marine organism, including from marine-derived fungi. Investigation of chemical compounds from marine-derived fungi isolated from sponge has increased steadily, indicating the important role of marine-derived fungi in the discovery of drug compounds. The sponge has produced various kinds of fungi, which have reported to provide a variety of pharmacologically active metabolites and structurally diverse. Study literature showed that many fungal genera isolated from the marine sponge dominated the genus Acremonium, Aspergillus, Penicillium, Phoma, and Fusarium. The high proportion of genera and new compounds showed that the fungi isolated from the sponge could develop.
Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia Tanod, Wendy Alexander; Dewanto, Didit Kustantio; Ndobe, Samliok; Riyadi, Putut Har; Putra, Masteria Yunovilsa
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 2 (2019): August 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5379.021 KB) | DOI: 10.15578/squalen.v14i2.394

Abstract

This study aimed to evaluate the potential antibacterial and antioxidant activities of Sinulariasp. and Sarcophyton sp. from the Palu Bay, Central Sulawesi, Indonesia. Soft corals were identified as Sinulariasp. (SC1), Sinularia sp. (SC2), andSarcophytonsp. (SC3). Antibacterial activity was examined using agar diffusion well method. Antioxidant activity was measured by the DPPH radical scavenging method. The samples were macerated in MeOH: DCM. The crude extracts were partitioned with DCM, EtOAc, and BuOH. The crude extract of Sinulariasp. (SC2) showed a very strong antibacterial activity as it was able to inhibit the growth of Staphylococcus aureusand Escherichia coliup to 10 mg/mL. Sinularia sp. (SC1) crude extract showed strong activity againstS. aureus, whereas it showed moderate against E. coli.Sarcophyton sp. (SC3) crude extract showed moderate activity against S. aureus, whereas it showed weak against E. coli. The partition fractions of the three soft coral extracts had the potential to be a potent antioxidant agent.
Co-Authors Adel, Yeldi S Erawati, Erawati Muliadin, Muliadin Nahar, Hendrik NUR FADILAH Putra, Masteria Yunovilsa Putut Har Riyadi Samliok Ndobe Wendy Alexander Tanod