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All Journal IPTEK The Journal for Technology and Science
Rachma, Fanina Aulia
Departement of Chemical Engineering Institut Teknologi Sepuluh Nopember, Surabaya
Computer Science & IT

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Study of UV-B Mutation Effect on pH Resistance and Lipid Production of Microalgae Botryococcus braunii Soedarmodjo, Thea Prastiwi; Rachma, Fanina Aulia; Aparamarta, Hakun Wirawista; Widjaja, Arief
IPTEK The Journal for Technology and Science Vol 30, No 3 (2019)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v30i3.5475

Abstract

Microalgae Botryococcus braunii is a potential biodiesel producer as an alternative for fossil fuels due to its high lipid content. UV-B mutations were carried out to see the effect in microalgae growth at various pHs (3-8). Reduction of nitrogen levels was carried out to see the effect on the growth and lipid production of microalgae. UV-B mutation increased the ability of growth and resistance of B. braunii against low pH. Under low nitrogen conditions, the growth of B. braunii cells would not continue for a longer time. B. braunii which grow in nitrogen depletion medium produced lipid content greater than normal nitrogen. UV-B light mutation also increased the lipid content of B. braunii. At 7 days of incubation, the mutation not only increased lipid content, but also significantly increased the TAG content of B. braunii lipids.
Co-Authors Aparamarta, Hakun Wirawista Sjarief Widjaja Soedarmodjo, Thea Prastiwi