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All Journal Microbiology Indonesia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia
Angky Budianti
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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EVIDENCE BASED CASE REPORT: PENGGUNAAN METODE PCR PADA SPUTUM UNTUK DIAGNOSIS PULMONARY ASPERGILLOSIS Augustine Natasha; Ricky Fernando; Tubagus M. Kurniadi; Elyn Dohar Idarin Aritonang; Angky Budianti
Majalah Kedokteran Indonesia Vol 69 No 6 (2019): Journal of the Indonesian Medical Association Majalah Kedokteran Indonesia Volum
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/jinma.v69i6.198

Abstract

Latar belakang: Pulmonary Aspergillosis merupakan infeksi oportunistik pada populasi immunocompromised. Metode Polymerase Chain Reaction (PCR) diharapkan dapat menjadi pengganti kultur jamur sputum dengan hasil yang lebih cepat dan akurat. Metode: Dilakukan pencarian di PubMed, CENTRAL, EbscoHost, dan ProQuest sejak tanggal 1 -13 Oktober menggunakan kata kunci ?Aspergillosis?, ?Pulmonary Aspergillosis?, ?sputum?, ?PCR?, ?sputum fungal culture?. Hasil pencarian di evaluasi menggunakan kriteria inklusi dan eksklusi. Studi yang terseleksi kemudian diperoleh full text nya dan dievaluasi kembali. Hasil akhir dari seleksi kemudian ditinjau secara kritis validity, importance, dan applicability-nya oleh ketiga penulis. Hasil: Didapatkan empat studi,dengan level of evidence 4, yang kemudian ditinjau secara kritis. Tiga studi menggunakan PCR pada sampel sputum sebagai index test, sementara satu studi menggunakannya sebagai refference test. Luaran dari tiga studi melaporkan sensitivitas dan spesifisitas dari PCR dengan sampel sputum, sedangkan satu studi melaporkan proporsi dari hasil PCR. Dua studi melaporkan sensitivitas PCR pada sampel sputum sebesar 100%, sementara satu studi melaporkan sensitivitas sebesar 38%. Dua studi juga melaporkan spesifisitas diatas 70%, sementara satu studi melaporkan spesifisitas sebesar 38.5%.Kesimpulan: Studi menunjukkan nilai sensitivitas dan spesifisitas yang menjanjikan terutama dengan hasil diperoleh dengan cepat. Akan tetapi rendahnya level of evidence dan biayanya yang cukup mahal, menyebabkan deteksi dengan metode PCR belum dapat digunakan sebagai modalitas diagnosis rutin.
Expression of Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in Mammalian Cell Line FITRIYAH SJATHA; OCTAVIA CHANDRA MUSTIKA; ANGKY BUDIANTI; TJAHJANI MIRAWATI SUDIRO
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1505.897 KB) | DOI: 10.5454/mi.13.1.5

Abstract

Dengue infection is a global infectious disease with almost 100 million cases occur annually in over more than 100 endemic countries. Dengue virus (DENV), the causative agent of dengue infection, is an 11 kbp RNApositive strand virus which encode 3 structural and 7 non-structural protein within its genome. Non-structural 1 (NS1) protein of DENV is expressed in the earlier stage of infection and having pathogenic role in disease severity. NS1 gene of DENV serotype-2 Indonesian strain was amplified through PCR method using specific designated primers. NS1 amplicon were then cloned into pUMVC4.a and pcDNA3.1 mammalian expression vector which confirmed through colony PCR and sequencing method. Recombinant pUNS1 and pcNS1 plasmids were transfected into CHO-K1 mammalian cell line with lipid based method. Recombinant NS1 protein expression were analyzed through immunostaining using dengue patient sera and rapid NS1 detection kit. Recombinant pUNS1 and pcNS1 plasmids were successfully constructed and recombinant NS1 protein was expressed in CHOK1 mammalian cell line and shown to be reactive against dengue patient sera. Our recombinant NS1 protein also tend to be released outside the transfected CHO-K1 cells as detected in rapid NS1 detection kit. Recombinant dengue NS1 protein was expressed in mammalian cell line in both intra and extracellularly and shown to be immunogenic.
Co-Authors Augustine Natasha Elyn Dohar Idarin Aritonang FITRIYAH SJATHA OCTAVIA CHANDRA MUSTIKA Ricky Fernando Tjahjani Mirawati Sudiro Tubagus M. Kurniadi